Detailed information of component protein


Summary

Component ID T6CP011043
Component protein type TssP
T6SS ID (Type) T6SS00907 experimental (Type iii)
Strain Bacteroides fragilis NCTC 9343
Replicon chromosome
Sequence Protein sequence (298 a.a.); Nucleotide sequence (894 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BF9343_RS09365
Coordinate (Strand) 2346972..2347865 (-)
NCBI ID 499302017
RefSeq NC_003228
Uniprot ID Q5LDV4_BACFN
KEGG ID bfs:BF9343_1920
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
PKD_4 PF18911.2 2.2e-07 40..96
PKD PF00801.22 1e-05 143..180
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-9 MMNKFLNTK
Transmembrane helix 10-27 VLYIFISVIILIMFFLLV
Inside 28-297 MRACSQKQPIQATVTPSDPVVGEEIFFSDSTSGARIWYWEFGNNETSTQRSGYHRFKQKGVYKIRLTVNGNLERYFDVRVKEKTNAEDLHLVHIIAPKEAIQGENIIFRGEGHDEQWRWEFGETGMIDSREKTALYAYTEPGEYEVLLNTENTRYPIRHRINILPYYSENDSTDVMVLIGLDIKEKLQNIADGKPFNVNYNYVVDKYFNNNPNTLVIVNNNKYNDFYSYCQGLHHIGRKETIIQNVIVETEDEESGYITQITVMQIEKKK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.5880 pos: 30-31. MRA-CS. MMNKFLNTKVLYIFISVIILIMFFLLVMRA


  Protein sequence: 298 a.a.    

>T6CP011043 NC_003228:c2347865-2346972 [Bacteroides fragilis NCTC 9343] [TssP]
MMNKFLNTKVLYIFISVIILIMFFLLVMRACSQKQPIQATVTPSDPVVGEEIFFSDSTSGARIWYWEFGNNETSTQRSGY
HRFKQKGVYKIRLTVNGNLERYFDVRVKEKTNAEDLHLVHIIAPKEAIQGENIIFRGEGHDEQWRWEFGETGMIDSREKT
ALYAYTEPGEYEVLLNTENTRYPIRHRINILPYYSENDSTDVMVLIGLDIKEKLQNIADGKPFNVNYNYVVDKYFNNNPN
TLVIVNNNKYNDFYSYCQGLHHIGRKETIIQNVIVETEDEESGYITQITVMQIEKKK*

  Nucleotide sequence: 894 bp    

>T6CP011043 NC_003228:c2347865-2346972 [Bacteroides fragilis NCTC 9343] [TssP]
ATGATGAATAAATTCCTAAATACGAAGGTTCTCTATATCTTTATCTCCGTTATCATACTTATTATGTTTTTTCTGCTGGT
AATGAGAGCTTGCTCGCAGAAGCAGCCGATTCAGGCTACTGTAACTCCGTCTGACCCTGTCGTGGGGGAAGAAATATTTT
TTTCCGATTCCACGTCGGGAGCGAGAATCTGGTACTGGGAGTTCGGAAACAACGAGACATCCACACAACGCAGCGGATAC
CATCGGTTCAAGCAAAAAGGGGTGTACAAAATACGACTGACCGTCAACGGAAATCTGGAACGCTACTTTGATGTGAGAGT
AAAAGAGAAGACCAATGCGGAAGACCTGCATCTGGTTCATATCATAGCTCCCAAGGAAGCCATTCAGGGAGAAAATATCA
TCTTCCGTGGCGAAGGACACGACGAACAATGGCGCTGGGAGTTCGGAGAGACGGGGATGATTGATTCCCGTGAAAAAACG
GCTCTTTATGCTTATACCGAACCCGGAGAGTACGAAGTATTGCTTAATACGGAGAATACCCGGTATCCCATCAGACACCG
GATAAACATTCTGCCCTACTATTCGGAGAATGATTCTACCGATGTAATGGTGCTCATCGGTTTAGACATCAAAGAGAAGT
TGCAGAACATTGCAGACGGTAAACCCTTTAATGTAAACTACAACTATGTCGTGGACAAGTATTTTAATAATAACCCGAAC
ACGCTGGTTATTGTCAACAACAATAAATATAACGACTTTTATTCTTATTGCCAAGGACTGCACCATATCGGCAGAAAAGA
AACGATTATCCAGAATGTCATCGTAGAGACGGAGGATGAAGAGAGCGGATATATTACCCAAATAACGGTTATGCAAATCG
AAAAAAAGAAATGA