SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011034
Component protein type	TssJ
T6SS ID (Type)	T6SS00905 (Type i1)
Strain	Escherichia coli strain TW-XM
Replicon	chromosome
Sequence	Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference	[1] Ma J, Bao Y, Sun M, et al. Two functional type VI secretion systems in avian pathogenic Escherichia coli are involved in different pathogenic pathways. Infect Immun. 2014 Sep;82(9):3867-79. doi: 10.1128/IAI.01769-14. Epub 2014 Jun 30. PMID: 24980972

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	12505..13029 (-)
NCBI ID	611963711
GenBank	KF678350
Uniprot ID	Q6TKU4_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.7e-23	45..164

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-174	MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFHAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLVHIRASAIEMKKEEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9991	pos: 18-19. LTG-CE.	MNGKAFLACVLMSVVLTG

Protein sequence: 175 a.a.

						>T6CP011034 KF678350:c13029-12505 [Escherichia coli] [TssJ]
MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKF
HAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLV
HIRASAIEMKKEEN*					

Nucleotide sequence: 525 bp

						>T6CP011034 KF678350:c13029-12505 [Escherichia coli] [TssJ]
ATGAACGGCAAAGCGTTTCTGGCCTGCGTTCTGATGAGCGTCGTATTAACTGGCTGTGAAACAGCGAAAAAAATCAGCCA
GGTGATCCGCAATCCGGATATTCAGGTCGGAAAGCTGATGGATCAGTCAACCGAGCTGACCGTCACGCTGCTGACCGAGC
CGGACAGCAACCTGACGGCGGATGGCGAAGCCGCGCCGGTGGATGTCCAGTTGGTTTATCTGAGCGACGACTCAAAATTC
CATGCCGCCGACTACGACCAGGTTGCCACCACCGCGCTGCCCGACGTGCTGGGGAAAAACTATATCGATCACCAGGACTT
CAACCTGTTGCCGGATACCGTAAAAACACTGCCGCCGATCAAGTTGGATGAGAAAACCGGTTATATCGGTGTCATTGCCT
ATTTTTCAGACGACCAGGCCACAGAATGGAAACAAATTGAGTCGGTAGAAAGTATCGGCCACCACTATCGCCTGCTGGTG
CATATCCGCGCCAGTGCGATTGAGATGAAAAAAGAGGAAAACTGA					