Detailed information of component protein
Summary
| Component ID | T6CP011024 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00904  (Type i2) |
| Strain | Escherichia coli strain TW-XM |
| Replicon | chromosome |
| Sequence | Protein sequence (135 a.a.); Nucleotide sequence (405 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 27410..27814 (+) |
| NCBI ID | 611963699 |
| GenBank | KF678349 |
| Uniprot ID | X5FV35_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 7.4e-20 | 49..133 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-134 | MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYKQIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKDVLAKHTVWVKPEGTASLNVPLDKETQFVAIIG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9983 | pos: 23-24. LSG-CG. | MFPIRFKRPALLCMAMLTVVLSG |
Protein sequence: 135 a.a.
>T6CP011024 KF678349:27410-27814 [Escherichia coli] [TssJ]
MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYKQIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTR
EDFDKADYDTLFMQEEKTLEKDVLAKHTVWVKPEGTASLNVPLDKETQFVAIIG*
MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYKQIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTR
EDFDKADYDTLFMQEEKTLEKDVLAKHTVWVKPEGTASLNVPLDKETQFVAIIG*
Nucleotide sequence: 405 bp
>T6CP011024 KF678349:27410-27814 [Escherichia coli] [TssJ]
ATGTTTCCTATTCGTTTTAAACGTCCGGCGTTGCTCTGTATGGCGATGCTGACGGTTGTTCTGAGTGGCTGCGGGCTGAT
TCAGAAAGTGGTGGATGAATCGAAAAGCGTGGCCTCAGCCGTTTTCTACAAACAAATCAAAATACTGCATCTCGATTTCT
TCTCCCGCAGCGCCCTGAATACGGATGCGGAAGATACGCCGCTTTCCACGATGGTGCATGTCTGGCAACTGAAAACCCGC
GAAGATTTTGACAAGGCGGATTACGACACCCTGTTTATGCAGGAAGAGAAGACGCTGGAGAAGGACGTACTGGCAAAACA
CACCGTCTGGGTAAAACCGGAAGGCACGGCATCCCTGAATGTGCCGCTGGATAAAGAGACGCAGTTTGTCGCCATTATTG
GGTAG
ATGTTTCCTATTCGTTTTAAACGTCCGGCGTTGCTCTGTATGGCGATGCTGACGGTTGTTCTGAGTGGCTGCGGGCTGAT
TCAGAAAGTGGTGGATGAATCGAAAAGCGTGGCCTCAGCCGTTTTCTACAAACAAATCAAAATACTGCATCTCGATTTCT
TCTCCCGCAGCGCCCTGAATACGGATGCGGAAGATACGCCGCTTTCCACGATGGTGCATGTCTGGCAACTGAAAACCCGC
GAAGATTTTGACAAGGCGGATTACGACACCCTGTTTATGCAGGAAGAGAAGACGCTGGAGAAGGACGTACTGGCAAAACA
CACCGTCTGGGTAAAACCGGAAGGCACGGCATCCCTGAATGTGCCGCTGGATAAAGAGACGCAGTTTGTCGCCATTATTG
GGTAG