Detailed information of component protein
Summary
| Component ID | T6CP011016 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00904  (Type i2) |
| Strain | Escherichia coli strain TW-XM |
| Replicon | chromosome |
| Sequence | Protein sequence (222 a.a.); Nucleotide sequence (666 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 3450..4115 (+) |
| NCBI ID | 611963682 |
| GenBank | KF678349 |
| Uniprot ID | X5FDR9_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 8e-45 | 9..207 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-181 | MKKDMDINIDALLRDTFLTVVELRQGTTVRHGIELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVAPFGVLPETAVLNVPLSTRQHP |
| Transmembrane helix | 182-204 | LLHSPFFWLVTLALLLAGVWWGL |
| Outside | 205-221 | HHWLNVLVDELLPQSLR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9843 | - | - |
Protein sequence: 222 a.a.
>T6CP011016 KF678349:3450-4115 [Escherichia coli] [TssL]
MKKDMDINIDALLRDTFLTVVELRQGTTVRHGIELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSG
MDDGQAIWMKNPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQSLR*
MKKDMDINIDALLRDTFLTVVELRQGTTVRHGIELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSG
MDDGQAIWMKNPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQSLR*
Nucleotide sequence: 666 bp
>T6CP011016 KF678349:3450-4115 [Escherichia coli] [TssL]
ATGAAAAAAGATATGGATATCAATATCGATGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAAC
CACCGTGCGTCACGGTATCGAGCTGTATCGCCACTGTCAGCGGCAGGTGGAACTGGTCCGCGAACGTCTGAAAGATGCCG
GATTCAGTCGGGAGAACGTGGAGCACATAACTTACGCGCAGTGCGCCCTGCTGGACGAAACGGTGCTCAGCCGTAGTGGC
ATGGACGACGGTCAGGCCATCTGGATGAAAAACCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCCGGTGAGCTGCT
GTACGAGCGGATGAAGCAGGTGCTACAGGAACCCGCCCCCGCGCAGGCCGTGCTGACCTGCTTTCACCGTGTCCTGCTGC
TGGGATTTCGCGGGCGTTATCAGGATCCGGCAGCCCCGGAGCGCGACCAGCTTATTTCGACGCTCAACGGGCAGGTCGCG
CCATTTGGTGTGCTGCCGGAAACGGCGGTACTGAACGTGCCGTTATCCACCCGTCAGCACCCTTTGCTGCATTCGCCATT
TTTCTGGCTGGTAACACTGGCGCTGCTACTGGCGGGCGTCTGGTGGGGGCTACACCACTGGCTGAATGTACTGGTGGATG
AACTGCTGCCGCAAAGCCTGCGTTAA
ATGAAAAAAGATATGGATATCAATATCGATGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAAC
CACCGTGCGTCACGGTATCGAGCTGTATCGCCACTGTCAGCGGCAGGTGGAACTGGTCCGCGAACGTCTGAAAGATGCCG
GATTCAGTCGGGAGAACGTGGAGCACATAACTTACGCGCAGTGCGCCCTGCTGGACGAAACGGTGCTCAGCCGTAGTGGC
ATGGACGACGGTCAGGCCATCTGGATGAAAAACCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCCGGTGAGCTGCT
GTACGAGCGGATGAAGCAGGTGCTACAGGAACCCGCCCCCGCGCAGGCCGTGCTGACCTGCTTTCACCGTGTCCTGCTGC
TGGGATTTCGCGGGCGTTATCAGGATCCGGCAGCCCCGGAGCGCGACCAGCTTATTTCGACGCTCAACGGGCAGGTCGCG
CCATTTGGTGTGCTGCCGGAAACGGCGGTACTGAACGTGCCGTTATCCACCCGTCAGCACCCTTTGCTGCATTCGCCATT
TTTCTGGCTGGTAACACTGGCGCTGCTACTGGCGGGCGTCTGGTGGGGGCTACACCACTGGCTGAATGTACTGGTGGATG
AACTGCTGCCGCAAAGCCTGCGTTAA