Detailed information of component protein


Summary

Component ID T6CP010982
Component protein type TssF
T6SS ID (Type) T6SS00787 experimental (Type i1)
Strain Escherichia coli RS218
Replicon chromosome
Sequence Protein sequence (614 a.a.); Nucleotide sequence (1842 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 15794..17635 (-)
NCBI ID 356875284
GenBank JN837480
Uniprot ID G8IRM5_ECOLX
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 9.3e-123 6..609
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-613 VAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVERLMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPVQVCRNELIRSRSEKSSLTAQKVLPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNASSLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAGEYRLPLPDLWLEAAGFDNRDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLPDDFPINAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYAEPVRPDGRMAEYPLRASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVYWHHRTKTSLLHHGLEHSIAFVHADGSIPGSSRFNDDVITTSLICTNRMIPARLHTGDICVAVNKNPAVASFRNVTRPTLPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRTFDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGEIYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9951 - -


  Protein sequence: 614 a.a.    

>T6CP010982 JN837480:c17635-15794 [Escherichia coli RS218] [TssF]
VAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVERLMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYL
RPVPAMTVIAYEPKTDQLKVPVQVCRNELIRSRSEKSSLTAQKVLPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAGEYRLPLPDLWLEAAGFDNRDAL
LPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLPDDFPINAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYA
EPVRPDGRMAEYPLRASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVYWHHRTKTS
LLHHGLEHSIAFVHADGSIPGSSRFNDDVITTSLICTNRMIPARLHTGDICVAVNKNPAVASFRNVTRPTLPLWPVTDGD
MHWSLISAMNLNYLSLLDRETLIQILRTFDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDP
FICEGEIYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI*

  Nucleotide sequence: 1842 bp    

>T6CP010982 JN837480:c17635-15794 [Escherichia coli RS218] [TssF]
GTGGCTTTTGAAGAACGTTACTACCGCGAAGAGCTGGATTATCTTCGCCAGCTAGGCAAACTGCTGGCGCAGGAAAAACC
ACATCTGGCCCACTTTCTGGCCGAGAAAGAGGGTGATCCGGACGTGGAGCGTCTGATGGAAGCCTTTGCCTTTATGTCCG
GTAGCCTGCGCCAGAAACTTGAGGACGAATTCCCCGAGTTCACCCATGGGCTGATCCGTATGCTGTGGGCAAACTATTTA
CGCCCGGTTCCGGCCATGACGGTTATTGCCTATGAACCCAAAACCGATCAGTTAAAAGTCCCTGTTCAGGTCTGTCGTAA
TGAACTGATCAGAAGCCGCTCAGAAAAGTCCTCCCTGACTGCTCAGAAGGTGCTGCCTGACAATCATGATAAAATCGTTT
CTTCCGTAGCCTGCCATTTCACGCTGGCGCGCGATATCTGGCTGCAACCACTGCGCATTCTCGATACCCGCAACGCCAGC
AGCCTGAAAGAAGGCATCATAGATATCAGTTTTACCGCAGATAACAATGTCTCCCCCGCCATGCTTGACCTGAACAAAAT
CACGTTCTGGCTGGGTAATGAAGACGATTATACCCGACACCAGCTTTATCTGTGGTTTTGCGAATGCCTGATGGATGCGG
AACTGATTGCCGGAGAGTACCGCCTGCCGCTGCCGGATCTGTGGCTGGAGGCGGCGGGTTTTGACAACCGGGATGCTCTG
CTGCCGTGGCCGAAAAATGTCCACAGCGGCTACCGCGTGCTTCAGGAGTATTTCTGCTATCCCGAAGCCTTCTTCTTTTT
CCATCTGCGCGATGTTGCGCCGCTACCAGACGATTTCCCGATCAATGCGTTTACGCTGCGCCTGCACTTTAACCGCCCGT
TACCTGCCGACATCAAGCTGCGCCAGGATTCGCTGCGTCTGCACTGTACGCCAGCCATTAACCTGTTTACGCATTATGCG
GAACCCGTCAGGCCGGATGGGCGGATGGCGGAATACCCGCTGCGCGCCAGCCATAAGCATCCGGACGCCTATGACATTTT
TCAGGTCAGCACAGTCACCAGTAAAGTCAAAGTTTCAGGTACTGAAATCAGCCCAGGTAGCCAGGCGCGTGTCTGGCCCG
AATTTGAAAGCTTCCAGCACCAGATGGAGTACAGCCGCCAGCGAGAAGTCGTCTACTGGCATCACCGGACTAAAACGTCC
CTGCTTCACCACGGGCTTGAACACTCTATCGCCTTTGTCCATGCAGACGGTAGCATTCCGGGCAGCTCACGGTTTAACGA
TGACGTGATCACCACGTCATTAATCTGTACCAACCGTATGATCCCTGCCCGGCTGCATACCGGTGATATCTGCGTGGCCG
TCAACAAAAATCCGGCAGTGGCCTCATTTCGTAATGTCACGCGCCCGACACTGCCGCTCTGGCCAGTCACGGACGGCGAC
ATGCACTGGTCGCTGATTTCCGCGATGAACCTGAACTACCTCTCCCTGCTGGACAGGGAGACACTGATCCAGATCCTGCG
GACCTTCGACCTGCCAGGTGCTCACCATCCGCAGCGGGCCAGACTGTCACGCCAGAAACTGGATGCCATAGAAAAACTCG
AAACCAAACCTGTTGACAGGTTGTTTAAAGGCGTACCGGTTCGCGGGCTGGCGACCACGCTGTGGATACGTCCCGATCCG
TTTATCTGCGAAGGTGAAATTTATCTTCTGGGTACCGTGCTCTCGCACTTCTTTGCCCTCTACGCCAGCATCAATTCGTA
CCACTGTCTGAAAATCATTAACACGGAAAGTCAGGAGTCCTGGGAATGGCAGGAGAAAATGGGCCAGCACGCCCTGATAT
AG