SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010981
Component protein type	TssG
T6SS ID (Type)	T6SS00787 (Type i1)
Strain	Escherichia coli RS218
Replicon	chromosome
Sequence	Protein sequence (350 a.a.); Nucleotide sequence (1050 bp)
Reference	[1] Zhou Y, Tao J, Yu H, et al. Hcp family proteins secreted via the type VI secretion system coordinately regulate Escherichia coli K1 interaction with human brain microvascular endothelial cells. Infect Immun. 2012 Mar;80(3):1243-51. doi: 10.1128/IAI.05994-11. Epub 2011 Dec 19. PMID: 22184413

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	14781..15830 (-)
NCBI ID	356875283
GenBank	JN837480
Uniprot ID	G8IRM4_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.4e-85	23..335

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-349	MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQDISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYYLDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYALVGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRKVAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRERYLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLSDPASSRLGRTSFIGQPKVPPAITIRIRE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9872	-	-

Protein sequence: 350 a.a.

						>T6CP010981 JN837480:c15830-14781 [Escherichia coli RS218] [TssG]
MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQDISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNG
QYVLQTRFLGFSGSQSPLPGYYLDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRKVAIDPAHQNRLGVRNPKGKTAG
HIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRERYLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRL
SDPASSRLGRTSFIGQPKVPPAITIRIRE*					

Nucleotide sequence: 1050 bp

						>T6CP010981 JN837480:c15830-14781 [Escherichia coli RS218] [TssG]
ATGGCAGGAGAAAATGGGCCAGCACGCCCTGATATAGCGACAAAACCGCCGTTACCGGCGGATGTGCGCAACTGCAATTT
CTATGTGCTGATGGAGGCGCTGTACCGTCGGCATGGCGCGCCGGGCCAGGACATTTCCCTGCGTACCGAGCCTGCCCGGG
AGATCGTGCGTTTCAGCTCTGACGCCAGTATCAGTTTTCCGGGTACGGACCTGAGTGCGCTTTCCCGTAGCCAGAACGGA
CAGTATGTACTGCAAACCCGGTTCCTGGGTTTTTCCGGCAGCCAGTCGCCGCTACCGGGCTATTATCTGGATCAGATGGC
GCAGGAGTCCGCACAGAACGAAGACGGCCTGAAAGAATTTCTCGATCTATTCAGCCACCGCTGGACACAGTTTGCCTATC
ATGCCTGGCGCAAATACCGCTACTACATCTGCTTTCGTAGCGGCGGTACTGACACCTTTTCACAGCGCATGTACGCACTG
GTCGGGCTGGGTAATCAGAGCGTGCGTGACAGGCTTGCCATTAACCACAGCAAAATGCTGGCCTACGCCGGAATTCTGGC
AACACCAGGCCGCGCGCCGGAGGTTATCTGTAATCTGGTGTCGCACTGTTTTGATTTGCCGGATGTCACCATTGAACGCT
GGCAACTGCGTAAAGTCGCCATTGATCCGGCACACCAGAACCGGCTGGGTGTACGTAATCCGAAAGGAAAAACGGCTGGA
CATATTCCGGGACGCTCCATACTCGGCGTTAATTTTACCCTCGGCGCACGGGTGCCGGATCGCAGCGGAAAATTCCTGTT
GCAGATTGGCGGCCTGTCGCGGGAGCGTTATCTCTCATTTTTACCCGATGGCGAAAATCACCTGCCGCTGACCATGTTCC
TTTCCTTTATTCTGCGCGACCAGTTTGCCTGGGATTTACGGCTGTGCCTGGCGCCACAGCAGGCTAAAGGGATGCGCCTT
AGCGATCCGGCCAGCTCCCGCCTCGGCAGAACCAGCTTTATTGGTCAGCCGAAAGTACCGCCCGCCATCACAATCCGAAT
CAGGGAATAA					