Detailed information of component protein


Summary

Component ID T6CP010980
Component protein type TssJ
T6SS ID (Type) T6SS00787 experimental (Type i1)
Strain Escherichia coli RS218
Replicon chromosome
Sequence Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) -
Coordinate (Strand) 12962..13486 (-)
NCBI ID 356875280
GenBank JN837480
Uniprot ID G8IRM1_ECOLX
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 4.7e-23 45..164

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-174 MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFHAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLVHIRASAIEMKKEEN



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9991 pos: 18-19. LTG-CE. MNGKAFLACVLMSVVLTG



  Protein sequence: 175 a.a.    

>T6CP010980 JN837480:c13486-12962 [Escherichia coli RS218] [TssJ]
MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKF
HAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLV
HIRASAIEMKKEEN*

  Nucleotide sequence: 525 bp    

>T6CP010980 JN837480:c13486-12962 [Escherichia coli RS218] [TssJ]
ATGAACGGCAAAGCGTTTCTGGCCTGCGTTCTGATGAGCGTCGTATTAACTGGCTGTGAAACAGCGAAAAAAATCAGCCA
GGTGATCCGCAATCCGGATATTCAGGTCGGAAAGCTGATGGATCAGTCAACCGAGCTGACCGTCACGCTGCTGACCGAGC
CGGACAGCAACCTGACGGCGGATGGCGAAGCCGCGCCGGTGGATGTCCAGTTGGTTTATCTGAGCGACGACTCAAAATTC
CATGCCGCCGACTACGACCAGGTTGCCACCACCGCGCTGCCCGACGTGCTGGGGAAAAACTATATCGATCACCAGGACTT
CAACCTGTTGCCGGATACCGTAAAAACACTGCCGCCGATCAAGTTGGATGAGAAAACCGGTTATATCGGTGTCATTGCCT
ATTTTTCAGACGACCAGGCCACAGAATGGAAACAAATTGAGTCGGTAGAAAGTATCGGCCACCACTATCGCCTGCTGGTG
CATATCCGCGCCAGTGCGATTGAGATGAAAAAAGAGGAAAACTGA