Detailed information of component protein
Summary
| Component ID | T6CP010978 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00787  (Type i1) |
| Strain | Escherichia coli RS218 |
| Replicon | chromosome |
| Sequence | Protein sequence (255 a.a.); Nucleotide sequence (765 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 10844..11608 (-) |
| NCBI ID | 356875278 |
| GenBank | JN837480 |
| Uniprot ID | G8IRL9_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 7.5e-55 | 34..234 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-213 | MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQAMPEHLFTQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGFRGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRR |
| Transmembrane helix | 214-236 | LTIKHVLCGGLVVLTVFYLFYLL |
| Inside | 237-254 | RLDSQTQDILHQLNKLLR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9150 | - | - |
Protein sequence: 255 a.a.
>T6CP010978 JN837480:c11608-10844 [Escherichia coli RS218] [TssL]
MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQAMPEHLFTQVVTDVQAVEQLLQEQG
YEPGVIVSFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKHVLCGGLVVLTVFYLFYLLRLDS
QTQDILHQLNKLLR*
MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQAMPEHLFTQVVTDVQAVEQLLQEQG
YEPGVIVSFRYILCTFIDEAALGNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKHVLCGGLVVLTVFYLFYLLRLDS
QTQDILHQLNKLLR*
Nucleotide sequence: 765 bp
>T6CP010978 JN837480:c11608-10844 [Escherichia coli RS218] [TssL]
ATGAGCGATATGAGTGAAGCGTCGGTTTTTCCCGTGGAGCCGGAGAGCGGAACACCAGGACGCCAGTACCGTCTCGCCCT
TCGCGGCAACAGCCTGAATCCAATGATTGACGCCGCCACGCCGTTGCTCGGCATGGTGATGCGTCTGTCCACTATGAACA
GTCAGGCCATGCCGGAACATCTGTTTACCCAGGTAGTAACCGATGTCCAGGCCGTAGAGCAACTACTCCAGGAACAGGGA
TACGAACCGGGCGTCATTGTTTCGTTCCGTTACATTCTCTGCACGTTTATTGATGAAGCGGCGCTGGGCAACGGCTGGTC
CAACAAAAACGAATGGATCAAACAGTCGCTGCTGGTTCATTTTCATAACGAAGCCTGGGGCGGCGAGAAAGTGTTTATCC
TGCTCGAACGCCTGATCCGGGAGCCGGTACGTTACCAGGATCTGCTGGAATTTTTATACCTCTGTTTTTCGCTCGGCTTT
CGCGGGCGCTACAAGGTTGCCATTCAGCAACAGGATGAATTTGAGCGGATCTACCAGCGCCTGCACCATGCCCTGCACAA
ACTGCGCGGTGACGCGCCATTTCCGCTACTTCATCAGAAGAACAACATACAGGGTGGGCGTTATCAGTTAATCCGACGAC
TGACCATCAAGCATGTACTGTGCGGCGGTCTTGTTGTGCTGACCGTATTTTATCTGTTTTATCTGCTGCGTCTGGACAGC
CAGACACAGGATATTCTGCATCAGCTCAATAAATTACTCCGATAA
ATGAGCGATATGAGTGAAGCGTCGGTTTTTCCCGTGGAGCCGGAGAGCGGAACACCAGGACGCCAGTACCGTCTCGCCCT
TCGCGGCAACAGCCTGAATCCAATGATTGACGCCGCCACGCCGTTGCTCGGCATGGTGATGCGTCTGTCCACTATGAACA
GTCAGGCCATGCCGGAACATCTGTTTACCCAGGTAGTAACCGATGTCCAGGCCGTAGAGCAACTACTCCAGGAACAGGGA
TACGAACCGGGCGTCATTGTTTCGTTCCGTTACATTCTCTGCACGTTTATTGATGAAGCGGCGCTGGGCAACGGCTGGTC
CAACAAAAACGAATGGATCAAACAGTCGCTGCTGGTTCATTTTCATAACGAAGCCTGGGGCGGCGAGAAAGTGTTTATCC
TGCTCGAACGCCTGATCCGGGAGCCGGTACGTTACCAGGATCTGCTGGAATTTTTATACCTCTGTTTTTCGCTCGGCTTT
CGCGGGCGCTACAAGGTTGCCATTCAGCAACAGGATGAATTTGAGCGGATCTACCAGCGCCTGCACCATGCCCTGCACAA
ACTGCGCGGTGACGCGCCATTTCCGCTACTTCATCAGAAGAACAACATACAGGGTGGGCGTTATCAGTTAATCCGACGAC
TGACCATCAAGCATGTACTGTGCGGCGGTCTTGTTGTGCTGACCGTATTTTATCTGTTTTATCTGCTGCGTCTGGACAGC
CAGACACAGGATATTCTGCATCAGCTCAATAAATTACTCCGATAA