Detailed information of component protein
Summary
| Component ID | T6CP010972 |
| Component protein type | TssM |
| T6SS ID (Type) | T6SS00787  (Type i1) |
| Strain | Escherichia coli RS218 |
| Replicon | chromosome |
| Sequence | Protein sequence (160 a.a.); Nucleotide sequence (480 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 5386..5865 (-) |
| NCBI ID | 356875272 |
| GenBank | JN837480 |
| Uniprot ID | G8IRL2_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-23 | VFKFPTSRLFSTLKSALRPAMPR |
| Transmembrane helix | 24-46 | FKVSATWLLTLAWIFLLVWIWWQ |
| Outside | 47-60 | GPKWTLYEQHWLAP |
| Transmembrane helix | 61-83 | LANRWLATAVWGLIALVWLTWRV |
| Inside | 84-159 | MKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLRLRRHLDNRRYLWQLPWYMVIGPAGSWQKHAAARGLSV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9925 | - | - |
Protein sequence: 160 a.a.
>T6CP010972 JN837480:c5865-5386 [Escherichia coli RS218] [TssM]
VFKFPTSRLFSTLKSALRPAMPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANRWLATAVWGLIALVWLT
WRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLRLRRHLDNRRYLWQLPWYMVIGPAGSWQKHAAARGLSV*
VFKFPTSRLFSTLKSALRPAMPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANRWLATAVWGLIALVWLT
WRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLRLRRHLDNRRYLWQLPWYMVIGPAGSWQKHAAARGLSV*
Nucleotide sequence: 480 bp
>T6CP010972 JN837480:c5865-5386 [Escherichia coli RS218] [TssM]
GTGTTCAAATTTCCCACATCCCGACTGTTCAGCACGTTGAAATCTGCGCTCAGGCCAGCGATGCCGCGGTTTAAAGTTTC
CGCCACCTGGCTACTGACGCTGGCATGGATTTTTCTGCTGGTGTGGATCTGGTGGCAGGGTCCAAAATGGACGCTCTATG
AGCAGCACTGGCTGGCTCCGCTGGCAAACCGCTGGCTGGCGACCGCCGTCTGGGGACTTATCGCTCTGGTCTGGCTCACC
TGGCGGGTGATGAAGCGTCTGCAAAAGCTGGAAAAACAGCAGAAACAGCAGCGGGAGGAAGAAAAAGATCCGTTGACCGT
GGAACTCCACCGCCAGCAGCAATATCTGGATCACTGGCTGCTGCGCCTGCGCCGCCATCTGGATAACCGCCGTTATCTGT
GGCAGTTGCCGTGGTATATGGTCATTGGTCCTGCGGGTAGCTGGCAAAAGCACGCTGCTGCGCGAGGGCTTTCCGTCTGA
GTGTTCAAATTTCCCACATCCCGACTGTTCAGCACGTTGAAATCTGCGCTCAGGCCAGCGATGCCGCGGTTTAAAGTTTC
CGCCACCTGGCTACTGACGCTGGCATGGATTTTTCTGCTGGTGTGGATCTGGTGGCAGGGTCCAAAATGGACGCTCTATG
AGCAGCACTGGCTGGCTCCGCTGGCAAACCGCTGGCTGGCGACCGCCGTCTGGGGACTTATCGCTCTGGTCTGGCTCACC
TGGCGGGTGATGAAGCGTCTGCAAAAGCTGGAAAAACAGCAGAAACAGCAGCGGGAGGAAGAAAAAGATCCGTTGACCGT
GGAACTCCACCGCCAGCAGCAATATCTGGATCACTGGCTGCTGCGCCTGCGCCGCCATCTGGATAACCGCCGTTATCTGT
GGCAGTTGCCGTGGTATATGGTCATTGGTCCTGCGGGTAGCTGGCAAAAGCACGCTGCTGCGCGAGGGCTTTCCGTCTGA