SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010960
Component protein type	TssJ
T6SS ID (Type)	T6SS00789 (Type i3)
Strain	Salmonella enterica GIFU10007
Replicon	chromosome
Sequence	Protein sequence (179 a.a.); Nucleotide sequence (537 bp)
Reference	[1] Wang M, Luo Z, Du H, et al. Molecular characterization of a functional type VI secretion system in Salmonella enterica serovar Typhi. Curr Microbiol. 2011 Jul;63(1):22-31. doi: 10.1007/s00284-011-9935-z. Epub 2011 Apr 13. PMID: 21487806

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	24545..25081 (-)
NCBI ID	AEC05358.1
GenBank	JF766712
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	6.6e-39	39..159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-178	VNEWRNPTRWLCAVAMPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYWSLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFIPGKGKVQLEAELEQSAIVITERDK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9989	pos: 25-26. LSG-CG.	VNEWRNPTRWLCAVAMPFALLLLSG

Protein sequence: 179 a.a.

						>T6CP010960 JF766712:c25081-24545 [Salmonella enterica] [TssJ]
VNEWRNPTRWLCAVAMPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYW
SLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFIPGKGKV
QLEAELEQSAIVITERDK*					

Nucleotide sequence: 537 bp

						>T6CP010960 JF766712:c25081-24545 [Salmonella enterica] [TssJ]
GTGAATGAATGGCGTAACCCCACTCGGTGGTTATGTGCGGTAGCTATGCCTTTTGCACTGCTCCTGCTTTCCGGATGCGG
CAGTAGCGATGCGTTACCTGACCTCGAATCACAGCGACTCGACCTGAGCGTGAAAGCCTCCGATAAGGTGAATCCTGACA
ATCAGAAGAAGGCCGCGCCCATTGAGATACGTGTTTATGAACTAAAAAATGACGCCGCTTTCACGACAGCTGATTACTGG
TCGCTCCATGACAACGACAAATCCGTCCTTACCGACGATTTAGTGCGTCGCGACAGCTTTATTTTGCGTCCCGGCGAAGA
GAAAAAACTGCGTCGCCCGCTGAATGCGCAGACCACGGCAATCGGCGTACTGGCCGGATACCGTAACCTGGCCAAATCGG
TCTGGCGGGTAACCTACAAAATCCCGGAAGCCCCGGAAAAAGCCTGGTACAGCAGCTTCATACCGGGGAAAGGAAAAGTG
CAGTTGGAGGCGGAACTGGAACAAAGCGCCATTGTAATTACGGAACGGGATAAATGA					