SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010953
Component protein type	TssJ
T6SS ID (Type)	T6SS00802 (Type Unknown)
Strain	Vibrio anguillarum NB10
Replicon	chromosome
Sequence	Protein sequence (156 a.a.); Nucleotide sequence (468 bp)
Reference	[1] Weber B, Hasic M, Chen C, et al. Type VI secretion modulates quorum sensing and stress response in Vibrio anguillarum. Environ Microbiol. 2009 Dec;11(12):3018-28. doi: 10.1111/j.1462-2920.2009.02005.x. Epub 2009 Jul 14. PMID: 19624706 [2] Weber B, Lindell K, El Qaidi S, et al. The phosphotransferase VanU represses expression of four qrr genes antagonizing VanO-mediated quorum-sensing regulation in Vibrio anguillarum. Microbiology (Reading). 2011 Dec;157(Pt 12):3324-3339. doi: 10.1099/mic.0.051011-0. Epub 2011 Sep 21. PMID: 21948044

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	6902..7369 (+)
NCBI ID	-
GenBank	GQ118992
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.7e-27	28..131

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-155	MVRRLCLMCIVLIMSGCSLWSYDMVEPKLVVDIKAATNINPNVEGKPSPVELRIYQLTNNNAFEISDFIQVYNDDQGVLKAELAVSRQLPSILPGETRQEIIPLGSGVKYIGVIAGFADYREAKNKVIYKPLIVNSAVISIEIDGINLSVSGEEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9886	pos: 16-17. MSG-CS.	MVRRLCLMCIVLIMSG

Protein sequence: 156 a.a.

						>T6CP010953 GQ118992:6902-7369 [Vibrio anguillarum] [TssJ]
MVRRLCLMCIVLIMSGCSLWSYDMVEPKLVVDIKAATNINPNVEGKPSPVELRIYQLTNNNAFEISDFIQVYNDDQGVLK
AELAVSRQLPSILPGETRQEIIPLGSGVKYIGVIAGFADYREAKNKVIYKPLIVNSAVISIEIDGINLSVSGEEE*					

Nucleotide sequence: 468 bp

						>T6CP010953 GQ118992:6902-7369 [Vibrio anguillarum] [TssJ]
ATGGTACGTCGTTTATGCCTAATGTGTATCGTCCTTATTATGTCAGGGTGCAGTTTATGGAGCTATGATATGGTTGAGCC
AAAACTGGTCGTTGATATTAAAGCTGCGACCAATATTAACCCTAATGTTGAGGGTAAGCCTTCTCCGGTAGAGCTCCGAA
TTTATCAATTGACGAATAATAATGCGTTTGAAATTTCTGATTTTATTCAAGTTTATAATGATGACCAAGGCGTTTTAAAG
GCCGAGTTGGCCGTTTCTCGCCAGCTACCCAGCATTTTGCCGGGCGAAACTCGTCAGGAAATTATCCCACTTGGTAGTGG
TGTTAAGTACATCGGTGTGATTGCGGGTTTTGCTGATTACAGAGAGGCGAAAAACAAGGTGATTTATAAGCCGCTAATTG
TGAATTCAGCGGTTATCAGTATTGAAATTGATGGCATTAATCTGTCTGTGTCAGGGGAAGAAGAATAA					