SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010906
Component protein type	TssJ
T6SS ID (Type)	T6SS00896 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Typhimurium
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Folkesson A, Löfdahl S, Normark S. The Salmonella enterica subspecies I specific centisome 7 genomic island encodes novel protein families present in bacteria living in close contact with eukaryotic cells. Res Microbiol. 2002 Oct;153(8):537-45. PMID: 12437215

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	14920..15411 (+)
NCBI ID	15130929
GenBank	AJ320483
Uniprot ID	Q93IS2_SALTM
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.7e-39	24..144

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-163	MPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYWSLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFISGKGKVQLEAELEQSAIVITERDK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.8443	pos: 10-11. LSG-CG.	MPFALLLLSG

Protein sequence: 164 a.a.

						>T6CP010906 AJ320483:14920-15411 [Salmonella enterica subsp. enterica serovar Typhimurium] [TssJ]
MPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYWSLHDNDKSVLTDDLV
RRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFISGKGKVQLEAELEQSAIVITE
RDK*					

Nucleotide sequence: 492 bp

						>T6CP010906 AJ320483:14920-15411 [Salmonella enterica subsp. enterica serovar Typhimurium] [TssJ]
ATGCCTTTTGCACTGCTCCTGCTTTCCGGATGCGGCAGTAGCGATGCGTTACCTGACCTCGAATCACAGCGACTCGACCT
GAGCGTGAAAGCCTCCGATAAGGTGAATCCTGACAATCAGAAGAAGGCCGCGCCCATTGAGATACGTGTTTATGAACTGA
AAAATGACGCCGCTTTCACGACAGCTGATTACTGGTCGCTCCATGACAACGACAAATCCGTCCTTACCGACGATTTAGTG
CGTCGCGACAGCTTTATTTTGCGTCCCGGCGAAGAGAAAAAACTGCGTCGCCCGCTGAATGCGCAGACCACGGCAATCGG
CGTACTGGCCGGATACCGTAACCTGGCCAAATCGGTCTGGCGGGTAACCTACAAAATCCCGGAAGCCCCGGAAAAAGCCT
GGTACAGCAGCTTCATATCGGGGAAAGGAAAAGTGCAGTTGGAGGCGGAACTGGAACAAAGCGCCATTGTAATTACGGAA
CGGGATAAATGA					