Detailed information of component protein


Summary

Component ID T6CP010896
Component protein type TssM
T6SS ID (Type) T6SS00897 experimental (Type i5)
Strain Rhizobium leguminosarum bv. trifolii
Replicon chromosome
Sequence Protein sequence (1159 a.a.); Nucleotide sequence (3477 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 13661..17137 (+)
NCBI ID 16326462
GenBank AF361470
Uniprot ID Q93EC2_RHILT
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.1e-71 207..444
IcmF-related PF06761.14 4.9e-57 498..794
IcmF_C PF06744.14 1.9e-20 1037..1139
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MNPLSYFYTLRSYVEAYAG
Transmembrane helix 20-42 LIGRRFISIIWVAAICVVIWFYG
Outside 43-56 YLAVYGDFKPLASA
Transmembrane helix 57-79 SARLTLIGIILAAWLAYLVFTTI
Inside 80-434 RDRRRDKQLVDGIERDAEAEAAASQQAEVGEIHGRLKEALQLLRRITKKRFGYIYELPWYVIFGAPGSGKTTALTNSGLKFPLGDALGSNSVQGIGGTRNCNWWFTDEAILIDTAGRYTTQDDLNGTAKAGWEGFLGLLRKYRRSQPINGALVTLSIGDLLTRDPEAQREEIRAIRQRLSELDELLHARVPVYLLLTKADFLTGFVEFFDGFNKSDREQVWGTTFGLDESYKAANLPERFMEEFTLLQQRVDTMLIERLQQEPNPELRGRIFRFPAELTSLKERLHEVVTELCSGSKLVEAPLLRGIYFASSTQREETVTVPRMRRSYFLSRLFKEVIFGEASLVARDKRLSGRQ
Transmembrane helix 435-457 LLFRRAAYASAVLLLAVVLTSWT
Outside 458-1158 ATYIQNTTALAGAERRIDAYEQLVRGVPVRDVSDADFLRILPALDNLAAVTTDFSKTRVWPISLGLDQEGKVASRQREAYQRALNTLLLPRMLVQLQKDMTETTDITRTFDALKLYGMLGGLGPVNADFASLEAEEMFTRLYPDEGRAAARQALIAHADVMARGALPPIELDKALIAKAREVIRSQNIANRAYDILAEYRESRALPAWSPAGALGPLGEQAFERTSKAPLNEGIPGLFSATGYRTVVLPGITDAAREALDEQWVRGDPNPAGVTVDTIAQATLQLYFDAFEQRWSMILTDIRVKPSQTIGDAAETTRILAGKPGPVETITKSIVAATDLRAPGAEIASAAGDSLPASTTALASAANAPDPFGRLRDALKAPAGQSSPDDQPKDAPRSEIGQLEPILQRVQEQLSRATTSTAEVAKVFDVDSQLTDANQDLLQQARKLPAPVDTWMAGLAADVGSLAVKSARSRISDAWAAEGASFCSDVVAGRYPFDRKSPRDVAMSDFIRLFGPEGLFKTFFKEKLEAFVDTSASPWGWKGTFGAVSIPSAAIAQFENADKINRAFFPAGSENPSISINVKPVSLTEAASAVMLEIEGERVVYFHGPIQSKSITWPSTDASNVSRLAFQPGGWQQALTENGDWSPFRLFDDADLAIQGDDLFRAKFRQNGQAAEFDVQFGSVLNPFRLEALGAFSCPAQF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9895 - -


  Protein sequence: 1159 a.a.    

>T6CP010896 AF361470:13661-17137 [Rhizobium leguminosarum bv. trifolii] [TssM]
MNPLSYFYTLRSYVEAYAGLIGRRFISIIWVAAICVVIWFYGYLAVYGDFKPLASASARLTLIGIILAAWLAYLVFTTIR
DRRRDKQLVDGIERDAEAEAAASQQAEVGEIHGRLKEALQLLRRITKKRFGYIYELPWYVIFGAPGSGKTTALTNSGLKF
PLGDALGSNSVQGIGGTRNCNWWFTDEAILIDTAGRYTTQDDLNGTAKAGWEGFLGLLRKYRRSQPINGALVTLSIGDLL
TRDPEAQREEIRAIRQRLSELDELLHARVPVYLLLTKADFLTGFVEFFDGFNKSDREQVWGTTFGLDESYKAANLPERFM
EEFTLLQQRVDTMLIERLQQEPNPELRGRIFRFPAELTSLKERLHEVVTELCSGSKLVEAPLLRGIYFASSTQREETVTV
PRMRRSYFLSRLFKEVIFGEASLVARDKRLSGRQLLFRRAAYASAVLLLAVVLTSWTATYIQNTTALAGAERRIDAYEQL
VRGVPVRDVSDADFLRILPALDNLAAVTTDFSKTRVWPISLGLDQEGKVASRQREAYQRALNTLLLPRMLVQLQKDMTET
TDITRTFDALKLYGMLGGLGPVNADFASLEAEEMFTRLYPDEGRAAARQALIAHADVMARGALPPIELDKALIAKAREVI
RSQNIANRAYDILAEYRESRALPAWSPAGALGPLGEQAFERTSKAPLNEGIPGLFSATGYRTVVLPGITDAAREALDEQW
VRGDPNPAGVTVDTIAQATLQLYFDAFEQRWSMILTDIRVKPSQTIGDAAETTRILAGKPGPVETITKSIVAATDLRAPG
AEIASAAGDSLPASTTALASAANAPDPFGRLRDALKAPAGQSSPDDQPKDAPRSEIGQLEPILQRVQEQLSRATTSTAEV
AKVFDVDSQLTDANQDLLQQARKLPAPVDTWMAGLAADVGSLAVKSARSRISDAWAAEGASFCSDVVAGRYPFDRKSPRD
VAMSDFIRLFGPEGLFKTFFKEKLEAFVDTSASPWGWKGTFGAVSIPSAAIAQFENADKINRAFFPAGSENPSISINVKP
VSLTEAASAVMLEIEGERVVYFHGPIQSKSITWPSTDASNVSRLAFQPGGWQQALTENGDWSPFRLFDDADLAIQGDDLF
RAKFRQNGQAAEFDVQFGSVLNPFRLEALGAFSCPAQF*

  Nucleotide sequence: 3477 bp    

>T6CP010896 AF361470:13661-17137 [Rhizobium leguminosarum bv. trifolii] [TssM]
ATGAACCCGCTCAGTTATTTCTATACGCTCCGCTCCTATGTGGAGGCCTATGCCGGCCTCATCGGCCGTCGCTTCATCTC
GATCATCTGGGTGGCGGCAATCTGTGTCGTCATCTGGTTCTACGGCTATCTCGCCGTCTATGGCGACTTCAAGCCGCTGG
CGAGCGCCAGCGCGCGGCTGACGCTGATCGGCATCATCCTCGCTGCCTGGCTCGCTTATCTCGTCTTCACGACCATCCGC
GACCGACGCCGCGACAAGCAGCTCGTCGACGGTATCGAGCGAGACGCGGAAGCAGAAGCCGCGGCTAGCCAACAGGCTGA
GGTCGGCGAGATCCACGGTCGGCTCAAGGAAGCGCTGCAGCTGCTCCGCCGCATCACCAAGAAGCGCTTCGGCTATATCT
ATGAGCTGCCATGGTACGTCATCTTCGGCGCGCCGGGGTCCGGCAAGACGACGGCGCTCACCAATTCCGGCCTCAAATTC
CCGCTCGGCGATGCGCTCGGCAGCAATTCGGTGCAGGGGATCGGCGGCACGCGCAACTGCAATTGGTGGTTCACCGACGA
GGCCATCCTCATCGACACGGCCGGCCGCTATACGACGCAGGACGATCTGAACGGCACCGCCAAGGCCGGCTGGGAAGGAT
TTCTCGGCCTGCTGCGCAAGTACCGTCGTTCTCAGCCGATCAACGGCGCCCTGGTGACGCTGTCGATCGGCGATCTCCTG
ACGCGCGATCCGGAAGCGCAGCGCGAGGAGATCAGGGCGATCCGCCAGCGGCTTTCCGAACTCGATGAACTCCTGCATGC
GCGCGTGCCGGTCTATCTGCTGCTGACCAAAGCCGATTTCTTGACCGGCTTCGTGGAGTTTTTCGACGGTTTCAACAAAA
GCGACCGCGAACAGGTCTGGGGCACGACGTTCGGGCTCGACGAGAGCTACAAGGCGGCGAACCTGCCGGAACGCTTCATG
GAGGAATTCACCCTGCTGCAGCAGCGCGTCGATACCATGCTGATCGAACGCCTGCAGCAGGAGCCTAATCCGGAATTGCG
CGGCCGCATCTTCCGTTTCCCGGCCGAACTCACCTCTTTGAAGGAGCGTCTGCACGAGGTCGTCACCGAGCTTTGCTCGG
GTTCCAAGCTGGTGGAGGCGCCGCTGCTGCGCGGCATCTATTTCGCTTCCAGCACCCAGAGGGAGGAGACAGTCACCGTG
CCCCGCATGCGGCGCAGCTATTTCCTCTCGCGCCTCTTCAAGGAGGTGATCTTCGGCGAGGCCTCGCTGGTCGCCCGCGA
CAAGCGGCTTTCGGGGCGGCAACTGCTCTTCCGCCGCGCGGCCTATGCGAGCGCGGTCCTGCTGCTTGCCGTGGTGTTGA
CCAGCTGGACGGCCACCTACATTCAGAACACCACGGCGCTGGCCGGCGCGGAAAGACGCATAGACGCCTACGAACAACTG
GTGCGTGGCGTGCCGGTTCGCGACGTTTCGGATGCGGATTTCCTGCGCATCCTGCCGGCGCTCGACAATCTCGCCGCCGT
CACCACCGACTTCTCGAAGACCCGCGTCTGGCCGATAAGCCTCGGCCTTGACCAGGAAGGCAAGGTCGCCAGCCGGCAGC
GCGAGGCCTATCAGCGGGCGCTGAACACGCTGCTCCTGCCGCGCATGCTGGTGCAGCTCCAGAAGGACATGACCGAGACC
ACCGACATCACCCGCACCTTCGACGCGTTGAAGCTCTACGGCATGCTCGGCGGGCTCGGCCCGGTGAATGCGGACTTTGC
CTCGCTGGAAGCAGAAGAGATGTTCACGAGGCTCTATCCCGACGAAGGCAGGGCGGCGGCCCGGCAGGCGCTGATTGCCC
ATGCCGATGTCATGGCGCGCGGCGCGCTGCCGCCGATCGAGCTCGACAAGGCGCTGATCGCCAAGGCGCGCGAGGTCATC
CGCTCGCAGAACATCGCCAACCGCGCCTATGACATCCTGGCGGAATATCGCGAGTCCCGCGCGCTTCCCGCCTGGAGCCC
GGCAGGCGCGCTGGGGCCGCTTGGCGAACAGGCTTTCGAGCGCACCTCGAAGGCGCCGCTCAACGAAGGCATTCCCGGCC
TCTTCTCCGCGACCGGCTATCGCACCGTCGTTCTGCCTGGGATCACCGACGCCGCGCGCGAGGCGCTCGACGAGCAATGG
GTCCGCGGCGACCCCAATCCCGCCGGCGTCACGGTCGACACGATCGCCCAGGCGACGCTTCAGCTTTACTTCGACGCATT
CGAGCAGCGCTGGTCGATGATCCTCACCGATATCAGGGTGAAGCCTTCCCAGACGATTGGCGATGCGGCGGAAACCACCC
GCATCCTGGCGGGCAAGCCCGGCCCGGTCGAGACGATCACCAAATCAATCGTCGCTGCCACCGATCTACGCGCGCCCGGG
GCGGAAATCGCCTCGGCCGCCGGCGACAGCCTGCCAGCCTCGACTACGGCGCTTGCCAGCGCAGCGAATGCGCCCGATCC
CTTCGGCCGTCTTCGAGATGCGCTGAAGGCGCCGGCCGGCCAATCGTCGCCGGACGACCAGCCGAAGGATGCGCCGCGAT
CGGAGATCGGGCAGCTCGAGCCGATCCTGCAGAGGGTCCAGGAGCAGCTTTCGCGCGCCACGACGTCGACGGCCGAAGTC
GCGAAAGTCTTCGACGTCGACAGCCAGCTCACCGACGCCAATCAGGACCTGCTGCAGCAGGCGCGCAAGCTCCCGGCGCC
GGTCGATACCTGGATGGCGGGGCTGGCTGCGGATGTCGGATCGCTGGCGGTCAAATCCGCCCGATCACGCATCAGCGACG
CCTGGGCTGCCGAAGGCGCGAGCTTCTGCTCGGATGTCGTCGCCGGTCGTTATCCTTTCGACCGAAAATCTCCGCGCGAC
GTGGCGATGAGCGATTTCATCAGGCTTTTCGGACCGGAGGGGCTGTTCAAGACCTTCTTCAAGGAAAAGCTCGAAGCCTT
CGTCGACACCAGCGCCTCGCCATGGGGCTGGAAGGGCACGTTCGGCGCGGTGAGCATTCCGAGTGCGGCGATTGCCCAGT
TCGAGAATGCCGACAAAATCAACCGCGCCTTCTTCCCGGCCGGCAGCGAGAACCCGTCGATTTCAATCAACGTCAAGCCG
GTATCGCTGACCGAGGCGGCCAGCGCCGTGATGCTGGAGATCGAGGGCGAACGGGTGGTCTATTTCCACGGGCCCATCCA
ATCGAAATCGATCACCTGGCCGTCGACGGACGCCAGCAACGTTTCGCGCCTCGCCTTCCAGCCGGGCGGCTGGCAGCAGG
CGCTGACGGAGAACGGCGACTGGTCGCCCTTCCGGCTCTTCGACGATGCCGATCTCGCCATACAGGGCGACGACCTCTTC
CGGGCAAAGTTCCGGCAGAACGGGCAGGCAGCAGAGTTCGACGTGCAGTTCGGTTCGGTGCTCAATCCATTCCGGCTGGA
GGCGCTCGGCGCCTTCTCATGCCCGGCGCAATTCTGA