Detailed information of component protein


Summary

Component ID T6CP010895
Component protein type TssL
T6SS ID (Type) T6SS00897 experimental (Type i5)
Strain Rhizobium leguminosarum bv. trifolii
Replicon chromosome
Sequence Protein sequence (511 a.a.); Nucleotide sequence (1533 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 12132..13664 (+)
NCBI ID 16326461
GenBank AF361470
Uniprot ID Q93EC3_RHILT
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3e-52 79..280
OmpA PF00691.22 4e-12 372..469
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-256 MKNEPASTWQNLPTIVELTEDGTRKKQSARKMAEMLDDVLDFPESEGGKTPAGSLPKGAASVETLVRDFRFGAEDVPTLVRSAAPLLNLAHGLRYTEEQPDIDELRQVAIEAVGRYERDLASARISPERARAAHYVICATVDDVVLSKAWGVRAGWARSGLVSTFHMDVTGGERVFDLLDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSPRGGLELGRIRDGLYKTLLGQYGVFERELSPHWKGVSARHQPLRT
Transmembrane helix 257-279 AAAIWTLLSLLTLILALGYMFFT
Outside 280-510 LSLNRASDGTFERLARLPPRDMPSVLVKVPEPPRQQPPVVVAETPPPPPPPQVKPPSRLDNLLAFLQPEVEKKLVTLSDSNGRLLVRISNSGLFATGSAEVSQKFHDLLQRIGGALAAENFRAVVVGYTDNVPIRTVQFPSNWHLSEARAKAVGEILSSFTGPGAILTEGRADTDPIAANDTPEGREMNRRTEILVLTDPSERLNDAGLTTPRRADMPDEPTAPAAGGGSR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9856 - -


  Protein sequence: 511 a.a.    

>T6CP010895 AF361470:12132-13664 [Rhizobium leguminosarum bv. trifolii] [TssL]
MKNEPASTWQNLPTIVELTEDGTRKKQSARKMAEMLDDVLDFPESEGGKTPAGSLPKGAASVETLVRDFRFGAEDVPTLV
RSAAPLLNLAHGLRYTEEQPDIDELRQVAIEAVGRYERDLASARISPERARAAHYVICATVDDVVLSKAWGVRAGWARSG
LVSTFHMDVTGGERVFDLLDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSPRGGLELGRIRDGLYKTLLGQYGVFEREL
SPHWKGVSARHQPLRTAAAIWTLLSLLTLILALGYMFFTLSLNRASDGTFERLARLPPRDMPSVLVKVPEPPRQQPPVVV
AETPPPPPPPQVKPPSRLDNLLAFLQPEVEKKLVTLSDSNGRLLVRISNSGLFATGSAEVSQKFHDLLQRIGGALAAENF
RAVVVGYTDNVPIRTVQFPSNWHLSEARAKAVGEILSSFTGPGAILTEGRADTDPIAANDTPEGREMNRRTEILVLTDPS
ERLNDAGLTTPRRADMPDEPTAPAAGGGSR*

  Nucleotide sequence: 1533 bp    

>T6CP010895 AF361470:12132-13664 [Rhizobium leguminosarum bv. trifolii] [TssL]
ATGAAGAACGAACCCGCCTCCACCTGGCAGAACCTGCCGACCATTGTCGAACTTACCGAGGACGGCACTCGGAAAAAGCA
ATCCGCCCGCAAGATGGCGGAGATGCTGGATGACGTCCTCGACTTTCCCGAAAGCGAGGGCGGAAAGACGCCGGCGGGAT
CGCTTCCGAAGGGCGCAGCCTCCGTCGAAACGCTCGTCCGGGATTTTCGCTTCGGAGCCGAAGACGTGCCGACGCTGGTG
CGCTCGGCCGCACCGCTCCTCAACCTCGCCCATGGCCTGCGCTACACGGAAGAACAGCCTGATATCGACGAACTGCGCCA
GGTGGCGATCGAAGCCGTCGGCCGCTACGAACGCGATCTGGCGAGCGCCCGCATCAGCCCCGAACGAGCGCGCGCCGCCC
ATTACGTCATCTGTGCGACGGTCGACGATGTCGTGCTCAGCAAGGCCTGGGGCGTTCGCGCCGGCTGGGCCCGCTCGGGG
CTCGTTTCCACCTTCCATATGGACGTGACCGGCGGCGAACGGGTGTTCGACCTGCTCGACCATTTCCACCAGAGCCCCGG
CGCCAACAAGGACCTGCTTCTCCTCATCTATCTCTGCCTGTCACTTGCCTTCGAGGGCCGCACCCGCGTTTCACCGCGCG
GCGGGCTGGAGCTCGGCCGCATCCGCGACGGCCTTTACAAGACGCTGCTTGGCCAGTACGGCGTCTTCGAACGAGAGCTT
TCTCCCCACTGGAAGGGCGTTTCGGCCCGGCATCAGCCGTTGCGCACCGCGGCCGCGATCTGGACGCTTCTTTCCCTTCT
GACGCTGATCCTGGCGCTCGGCTACATGTTCTTCACGCTCTCGCTCAATCGCGCCTCCGACGGAACGTTCGAGCGGCTCG
CCAGGCTGCCGCCGCGCGATATGCCGAGCGTACTCGTCAAGGTTCCGGAGCCGCCTCGGCAGCAGCCGCCCGTCGTCGTC
GCCGAAACGCCGCCGCCGCCGCCACCGCCGCAAGTGAAACCGCCGAGCCGGCTCGACAATCTGCTCGCCTTCCTCCAGCC
GGAGGTCGAGAAGAAGCTCGTCACACTCTCGGATTCGAATGGCAGGCTGCTGGTGCGGATCAGCAATTCCGGCCTGTTTG
CCACCGGCAGCGCGGAGGTCAGTCAAAAATTCCACGATCTTCTCCAGCGGATCGGCGGTGCGCTGGCCGCAGAGAACTTC
CGCGCCGTGGTGGTGGGTTATACCGACAATGTGCCGATCCGCACCGTGCAGTTCCCCTCCAACTGGCACCTTTCCGAAGC
CCGCGCCAAGGCGGTCGGCGAAATCCTGAGCTCCTTCACCGGGCCGGGTGCGATCCTGACCGAGGGGCGTGCCGACACCG
ATCCGATCGCCGCCAACGATACGCCGGAGGGCCGGGAGATGAACCGCCGCACCGAGATCCTGGTCCTGACCGATCCGAGC
GAGCGGCTGAACGATGCGGGTTTGACGACGCCGCGTCGGGCCGACATGCCGGATGAACCGACGGCGCCCGCCGCAGGAGG
TGGTTCGCGATGA