SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010893
Component protein type	TssG
T6SS ID (Type)	T6SS00897 (Type i5)
Strain	Rhizobium leguminosarum bv. trifolii
Replicon	chromosome
Sequence	Protein sequence (334 a.a.); Nucleotide sequence (1002 bp)
Reference	[1] Bladergroen MR, Badelt K, Spaink HP. Infection-blocking genes of a symbiotic Rhizobium leguminosarum strain that are involved in temperature-dependent protein secretion. Mol Plant Microbe Interact. 2003 Jan;16(1):53-64. PMID: 12580282

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	8593..9594 (+)
NCBI ID	16326484
GenBank	AF361470
Uniprot ID	Q93EC6_RHILT
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.5e-64	41..309

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-333	MDLPTARKPDALADLLERDPGRFEPVTAFRVAQASVGEGRLDVASHVGVSPAPLAVSGYKRKGGSATVRSALAGLVGALGSMPSAYNDLVMREERNRSRALAGFFDMFGARMAALFTDACEKYRIARRLRWGGAWANNAFVTTLLSLTGFGTKRLVEKSGVDEELILRFSGFFAARNRNAVNLRAMLCEFSGLPVEIELFRGRWLTIPIEERSRIGQPQGVQLGVNATAGAAIHDFSGGFRIVIGPLGYADYLSLAPGGRAITELFALARLYVGAALEFDIQAILKKEDVPFCQLGQAGDPPRLGWNSWARVAPAASDSGDAVIVERLSVQAR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9442	-	-

Protein sequence: 334 a.a.

						>T6CP010893 AF361470:8593-9594 [Rhizobium leguminosarum bv. trifolii] [TssG]
MDLPTARKPDALADLLERDPGRFEPVTAFRVAQASVGEGRLDVASHVGVSPAPLAVSGYKRKGGSATVRSALAGLVGALG
SMPSAYNDLVMREERNRSRALAGFFDMFGARMAALFTDACEKYRIARRLRWGGAWANNAFVTTLLSLTGFGTKRLVEKSG
VDEELILRFSGFFAARNRNAVNLRAMLCEFSGLPVEIELFRGRWLTIPIEERSRIGQPQGVQLGVNATAGAAIHDFSGGF
RIVIGPLGYADYLSLAPGGRAITELFALARLYVGAALEFDIQAILKKEDVPFCQLGQAGDPPRLGWNSWARVAPAASDSG
DAVIVERLSVQAR*					

Nucleotide sequence: 1002 bp

						>T6CP010893 AF361470:8593-9594 [Rhizobium leguminosarum bv. trifolii] [TssG]
ATGGATCTCCCGACCGCCCGCAAGCCAGACGCCCTTGCCGATCTGCTCGAACGCGACCCCGGCCGCTTCGAGCCGGTGAC
GGCCTTTCGCGTCGCGCAGGCTTCGGTAGGGGAAGGACGGCTAGATGTCGCCTCGCATGTCGGCGTCTCGCCGGCGCCTC
TTGCCGTCAGCGGATATAAGCGCAAGGGCGGCAGTGCCACCGTCCGCAGCGCGCTTGCCGGTCTCGTCGGGGCGCTCGGC
TCGATGCCGTCGGCCTATAACGACCTCGTCATGCGCGAGGAGCGCAACAGGTCGCGGGCGCTGGCGGGCTTCTTCGACAT
GTTCGGCGCGCGGATGGCGGCACTCTTCACTGACGCCTGCGAGAAATATCGTATCGCCAGGCGGCTGCGATGGGGCGGCG
CATGGGCAAACAACGCCTTCGTCACCACGCTCCTGTCACTCACCGGCTTCGGCACCAAACGTCTCGTCGAAAAGAGCGGC
GTCGACGAGGAACTGATCCTGCGTTTCTCGGGCTTCTTTGCCGCGCGCAACCGCAATGCCGTCAATCTCCGCGCCATGCT
CTGCGAATTCAGTGGCCTGCCGGTGGAGATCGAATTGTTCCGCGGCCGCTGGCTTACCATCCCGATCGAGGAGCGCAGCC
GCATCGGCCAGCCCCAGGGCGTCCAGCTTGGCGTCAATGCGACGGCAGGTGCTGCGATCCACGATTTCAGCGGCGGCTTT
CGCATCGTCATCGGTCCGCTCGGTTATGCCGACTACCTCTCGCTCGCGCCGGGCGGCCGCGCCATCACCGAACTCTTCGC
CCTGGCGCGGCTCTATGTCGGTGCGGCGCTCGAATTCGACATACAGGCGATCCTGAAGAAGGAGGACGTTCCCTTCTGCC
AGCTCGGACAGGCGGGCGATCCGCCGCGGCTTGGCTGGAACAGTTGGGCGAGGGTTGCGCCGGCTGCAAGCGACAGCGGC
GATGCAGTGATCGTCGAGCGCCTGTCGGTTCAGGCGCGGTGA					