SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010891
Component protein type	TssE
T6SS ID (Type)	T6SS00897 (Type i5)
Strain	Rhizobium leguminosarum bv. trifolii
Replicon	chromosome
Sequence	Protein sequence (170 a.a.); Nucleotide sequence (510 bp)
Reference	[1] Bladergroen MR, Badelt K, Spaink HP. Infection-blocking genes of a symbiotic Rhizobium leguminosarum strain that are involved in temperature-dependent protein secretion. Mol Plant Microbe Interact. 2003 Jan;16(1):53-64. PMID: 12580282

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	6289..6798 (+)
NCBI ID	16326482
GenBank	AF361470
Uniprot ID	Q93EC8_RHILT
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	8.1e-14	44..142

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-169	MVDPLERYRLRDRVLASSILDRLIDEAPDRTVDPPMSFVDQVRDVREAIRRDLEALLNTRRCPATPPAALSELRDALVSYGVDGVVSANLMTDQAKLKLAQAIERRIALFETRLSDVRVTILKSRTMTERALRMRIQATFRLHEGMPPISFESTIDPSTQRFLVEAAHG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9798	-	-

Protein sequence: 170 a.a.

						>T6CP010891 AF361470:6289-6798 [Rhizobium leguminosarum bv. trifolii] [TssE]
MVDPLERYRLRDRVLASSILDRLIDEAPDRTVDPPMSFVDQVRDVREAIRRDLEALLNTRRCPATPPAALSELRDALVSY
GVDGVVSANLMTDQAKLKLAQAIERRIALFETRLSDVRVTILKSRTMTERALRMRIQATFRLHEGMPPISFESTIDPSTQ
RFLVEAAHG*					

Nucleotide sequence: 510 bp

						>T6CP010891 AF361470:6289-6798 [Rhizobium leguminosarum bv. trifolii] [TssE]
ATGGTTGATCCGCTCGAACGCTACCGGCTGCGCGACCGCGTCCTTGCCAGCTCGATCCTCGACAGGTTGATCGACGAGGC
TCCGGATCGCACCGTCGATCCGCCGATGAGCTTCGTCGATCAGGTCCGCGACGTCAGGGAGGCGATCCGCCGCGATCTCG
AAGCTCTGCTCAATACGCGGCGCTGCCCGGCCACACCGCCGGCGGCACTTTCCGAACTCAGGGACGCGCTGGTGAGCTAT
GGCGTCGACGGCGTCGTCTCGGCAAATCTGATGACCGACCAGGCCAAGCTCAAGCTGGCGCAAGCGATCGAGCGACGGAT
TGCGCTTTTCGAAACCCGGCTTTCCGATGTGCGCGTGACGATCCTCAAGAGCCGGACGATGACCGAACGGGCGCTGCGCA
TGCGCATCCAGGCGACGTTCCGCCTGCATGAAGGCATGCCGCCGATCAGCTTTGAGTCGACCATCGATCCCTCGACCCAG
CGTTTCCTCGTGGAGGCGGCGCATGGCTGA					