SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010888
Component protein type	TssB
T6SS ID (Type)	T6SS00897 (Type i5)
Strain	Rhizobium leguminosarum bv. trifolii
Replicon	chromosome
Sequence	Protein sequence (182 a.a.); Nucleotide sequence (546 bp)
Reference	[1] Bladergroen MR, Badelt K, Spaink HP. Infection-blocking genes of a symbiotic Rhizobium leguminosarum strain that are involved in temperature-dependent protein secretion. Mol Plant Microbe Interact. 2003 Jan;16(1):53-64. PMID: 12580282

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1857..2402 (+)
NCBI ID	AAL17797.1
GenBank	AF361470
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	3.3e-60	16..168

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-181	MGRRRISMASVHEKLERVRKPRVHIKYEVETEGAMVVKELPFVVGVLGDFSGNPTQPLKPFGERKFVQVDRDNFDDVMRRMTPGLTLSVENTLEKNGTELPVSLKFESMADFEPGAVVDQVPGLKALLNARNQLRDLMSKADRSEELERLLEDILQNKADLSQLVAQLGGDKADEAKDALN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9891	-	-

Protein sequence: 182 a.a.

						>T6CP010888 AF361470:1857-2402 [Rhizobium leguminosarum bv. trifolii] [TssB]
MGRRRISMASVHEKLERVRKPRVHIKYEVETEGAMVVKELPFVVGVLGDFSGNPTQPLKPFGERKFVQVDRDNFDDVMRR
MTPGLTLSVENTLEKNGTELPVSLKFESMADFEPGAVVDQVPGLKALLNARNQLRDLMSKADRSEELERLLEDILQNKAD
LSQLVAQLGGDKADEAKDALN*					

Nucleotide sequence: 546 bp

						>T6CP010888 AF361470:1857-2402 [Rhizobium leguminosarum bv. trifolii] [TssB]
ATGGGACGAAGGAGAATCTCGATGGCAAGTGTGCATGAGAAACTGGAACGGGTTCGCAAACCGCGAGTCCACATCAAATA
CGAAGTGGAAACCGAAGGCGCGATGGTGGTGAAGGAACTGCCCTTCGTCGTCGGCGTGCTCGGCGACTTCTCCGGAAATC
CCACGCAGCCGCTCAAGCCTTTCGGCGAGCGCAAGTTCGTGCAGGTCGACCGCGACAACTTCGACGACGTCATGCGCCGC
ATGACGCCGGGCCTCACCCTGTCGGTCGAGAACACTCTCGAGAAGAACGGAACAGAGCTTCCCGTTTCGCTGAAATTCGA
AAGCATGGCCGATTTCGAACCCGGCGCGGTCGTCGATCAGGTGCCGGGGCTGAAGGCCCTGCTCAACGCGCGCAACCAGC
TGCGCGACCTGATGAGCAAGGCGGACCGCTCCGAAGAGCTCGAGCGCCTGCTTGAGGATATCCTGCAGAACAAGGCCGAT
CTCTCGCAGCTGGTCGCCCAGCTTGGCGGCGACAAAGCCGACGAGGCCAAGGACGCATTGAACTGA					