SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010887
Component protein type	TssA
T6SS ID (Type)	T6SS00897 (Type i5)
Strain	Rhizobium leguminosarum bv. trifolii
Replicon	chromosome
Sequence	Protein sequence (359 a.a.); Nucleotide sequence (1077 bp)
Reference	[1] Bladergroen MR, Badelt K, Spaink HP. Infection-blocking genes of a symbiotic Rhizobium leguminosarum strain that are involved in temperature-dependent protein secretion. Mol Plant Microbe Interact. 2003 Jan;16(1):53-64. PMID: 12580282

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	744..1820 (+)
NCBI ID	16326486
GenBank	AF361470
Uniprot ID	Q93ED3_RHILT
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	2.6e-25	8..133

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-358	VNVREIIDPLEQNHPCGENVRSNTAFREIYYRIKDARNAARTAERSIIPGETITIAPAWHDVSNLGLQLLSSKSKDIEVLAWLAEAQLRLRGFSGLHDVYVATVSLLDKHFDALHSIGDGDVEERFAPFAGLNGVGGEGTLIQAIRLTSLIPGGKFAQFSLWDFQLSQRPNETERRKELQQAAAEAGVAQMSAYLDVLTGCIAAFDRMVEILDEHCGDQAPPSSNTRNVLQEAASAIRMLAGIEAPTEAPLAAPQALASQGSEPLPASETDGSEAIGLRPVSAEMIRSREEAFELLIAVARYFRRTEPHSPISMSIETLVRRGRMDFFELLAELLPEQQTRNAVLTAAGIQPVADKGG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9889	-	-

Protein sequence: 359 a.a.

						>T6CP010887 AF361470:744-1820 [Rhizobium leguminosarum bv. trifolii] [TssA]
VNVREIIDPLEQNHPCGENVRSNTAFREIYYRIKDARNAARTAERSIIPGETITIAPAWHDVSNLGLQLLSSKSKDIEVL
AWLAEAQLRLRGFSGLHDVYVATVSLLDKHFDALHSIGDGDVEERFAPFAGLNGVGGEGTLIQAIRLTSLIPGGKFAQFS
LWDFQLSQRPNETERRKELQQAAAEAGVAQMSAYLDVLTGCIAAFDRMVEILDEHCGDQAPPSSNTRNVLQEAASAIRML
AGIEAPTEAPLAAPQALASQGSEPLPASETDGSEAIGLRPVSAEMIRSREEAFELLIAVARYFRRTEPHSPISMSIETLV
RRGRMDFFELLAELLPEQQTRNAVLTAAGIQPVADKGG*					

Nucleotide sequence: 1077 bp

						>T6CP010887 AF361470:744-1820 [Rhizobium leguminosarum bv. trifolii] [TssA]
GTGAACGTTCGCGAAATTATAGATCCACTTGAACAGAATCATCCGTGCGGAGAAAACGTGCGGAGTAATACTGCATTTCG
TGAAATATACTATAGAATTAAGGACGCGAGAAACGCCGCGCGTACAGCCGAACGTAGTATTATCCCGGGTGAGACTATTA
CGATTGCACCTGCTTGGCACGATGTTAGCAATCTTGGATTGCAGCTCCTTTCTTCAAAGAGCAAGGACATCGAAGTATTG
GCATGGCTCGCAGAGGCGCAGCTGCGGCTGCGCGGCTTCTCTGGATTGCATGACGTTTACGTCGCAACCGTGTCGCTTTT
AGACAAGCATTTCGACGCTTTGCATTCGATCGGAGATGGTGACGTCGAGGAACGGTTTGCGCCGTTTGCGGGACTGAACG
GCGTCGGCGGCGAAGGAACGCTTATCCAGGCTATTCGTTTGACATCGTTGATCCCCGGCGGAAAGTTCGCGCAGTTTTCG
CTTTGGGATTTCCAGCTTTCGCAGCGGCCGAACGAAACCGAACGACGCAAGGAATTGCAGCAGGCGGCAGCGGAAGCCGG
TGTCGCTCAGATGTCAGCCTATCTCGACGTGCTGACCGGATGCATCGCCGCTTTCGATCGCATGGTCGAAATACTTGACG
AACACTGCGGCGACCAGGCGCCACCGAGTTCGAATACACGCAATGTGTTGCAGGAGGCGGCATCTGCAATCCGGATGCTG
GCAGGAATCGAAGCGCCGACCGAAGCGCCCTTGGCCGCTCCGCAAGCCCTTGCCTCGCAAGGATCCGAGCCACTACCGGC
AAGCGAAACCGACGGGTCCGAAGCCATCGGCCTGCGGCCTGTCAGCGCCGAGATGATTCGTTCACGGGAAGAAGCCTTCG
AACTTCTGATCGCCGTTGCGCGCTATTTCCGCCGCACCGAGCCCCATTCACCGATCTCCATGTCGATCGAAACGCTGGTG
CGTCGCGGCCGCATGGATTTTTTCGAGCTTCTTGCCGAGCTCCTGCCCGAACAGCAGACGCGCAACGCGGTGCTGACGGC
AGCCGGCATCCAGCCCGTAGCCGACAAGGGAGGCTAA					