SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010095
Component protein type	TssI
T6SS ID (Type)	T6SS00838 (Type i1)
Strain	Proteus mirabilis BB2000
Replicon	chromosome
Sequence	Protein sequence (731 a.a.); Nucleotide sequence (2193 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Gibbs KA, Urbanowski ML, Greenberg EP. Genetic determinants of self identity and social recognition in bacteria. Science. 2008 Jul 11;321(5886):256-9. doi: 10.1126/science.1160033. PMID: 18621670 [3] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BB2000_RS03995
Coordinate (Strand)	941108..943300 (+)
NCBI ID	WP_020945430.1
RefSeq	NC_022000
Uniprot ID	UPI0003843CDE
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	9.3e-59	130..421
Phage_base_V	PF04717.14	2.1e-11	480..543

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-730	MLSKDPKSTPIDPASVLKDEAINQVKNRVQSKVNETLSTPTQKVAGMASAAASLTQSASASANRVTAHPLIHGGGFTGGQTFAENQALQAAHGALLDALGMDSLESGLVFTCTIGGLPDDTFQVTEFNLTEGLSSLFSLSISAVSALPFIDFQRHLGLASSLTVKRDGVLIRKVNGILAGAVQGNTDGVKTWYHFDIRPEMWVMTLNQDSRIFQHQSVPTILKTLLDEAHVKADCQFYREDLHPERLYTTQKRESAFDFWCRLAFEEGINFWFEEEQLFYSDEHMGMTAGIHLTYNPQADTDISDSTAMTWQYGEYLCVDETIHKDNNFVRPSYPLSHENKIEQGGQHSVFESYGRFQLDDEGRPLTQVRFEQLRNGSKVGQATTNCFALMPGKIFTLSQHPHQPMNDRWQVISVSHHGVQPLADNSGGEGTQLSNSVSFIPGTQEWRPPYRYKPTADGDEVATVVGPPGEEIYVNEYGAVKVHFHWDRRGSADHSASCWVRVAMGWSGNGYGFSAVPRIGTQVLVSYLNGDIDRPMITGCTYDGRNAPPIKFPENKTQTTFRSQTHKGEGFNELRFEDAGGKQEVFLHAQKDMNTVVQNDKGTTVGANHTETIMQNQKISVHGTQTTAVQADQKNIVFGNQHSIVDGEVLIASAKGIRLISGNSALQLNPDGTITLVCNNFDFYGHGSGRIGTGELLDLNMDGAGPGNLEMKPDTSTIAQAKDQFFPKK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.5396	-	-

Protein sequence: 731 a.a.

						>T6CP010095 NC_022000:941108-943300 [Proteus mirabilis BB2000] [TssI]
MLSKDPKSTPIDPASVLKDEAINQVKNRVQSKVNETLSTPTQKVAGMASAAASLTQSASASANRVTAHPLIHGGGFTGGQ
TFAENQALQAAHGALLDALGMDSLESGLVFTCTIGGLPDDTFQVTEFNLTEGLSSLFSLSISAVSALPFIDFQRHLGLAS
SLTVKRDGVLIRKVNGILAGAVQGNTDGVKTWYHFDIRPEMWVMTLNQDSRIFQHQSVPTILKTLLDEAHVKADCQFYRE
DLHPERLYTTQKRESAFDFWCRLAFEEGINFWFEEEQLFYSDEHMGMTAGIHLTYNPQADTDISDSTAMTWQYGEYLCVD
ETIHKDNNFVRPSYPLSHENKIEQGGQHSVFESYGRFQLDDEGRPLTQVRFEQLRNGSKVGQATTNCFALMPGKIFTLSQ
HPHQPMNDRWQVISVSHHGVQPLADNSGGEGTQLSNSVSFIPGTQEWRPPYRYKPTADGDEVATVVGPPGEEIYVNEYGA
VKVHFHWDRRGSADHSASCWVRVAMGWSGNGYGFSAVPRIGTQVLVSYLNGDIDRPMITGCTYDGRNAPPIKFPENKTQT
TFRSQTHKGEGFNELRFEDAGGKQEVFLHAQKDMNTVVQNDKGTTVGANHTETIMQNQKISVHGTQTTAVQADQKNIVFG
NQHSIVDGEVLIASAKGIRLISGNSALQLNPDGTITLVCNNFDFYGHGSGRIGTGELLDLNMDGAGPGNLEMKPDTSTIA
QAKDQFFPKK*					

Nucleotide sequence: 2193 bp

						>T6CP010095 NC_022000:941108-943300 [Proteus mirabilis BB2000] [TssI]
ATGCTTAGCAAAGATCCGAAATCAACGCCCATTGACCCGGCATCCGTTTTAAAAGATGAAGCCATCAATCAAGTGAAAAA
TAGGGTGCAATCCAAAGTGAACGAAACGCTATCGACACCCACGCAAAAGGTGGCCGGGATGGCCTCAGCAGCGGCCTCGC
TCACTCAATCAGCCAGCGCATCGGCCAATCGCGTGACGGCTCATCCCCTGATCCACGGTGGCGGTTTTACCGGTGGACAA
ACATTCGCTGAAAACCAAGCACTCCAAGCGGCCCATGGGGCGTTACTTGATGCATTAGGCATGGACAGCCTCGAAAGTGG
TTTGGTGTTCACTTGCACCATTGGTGGATTGCCTGACGACACCTTTCAAGTCACCGAATTTAATCTTACCGAAGGATTAT
CCTCGCTATTTTCGCTGTCCATCAGTGCGGTCAGTGCCTTACCCTTTATCGATTTTCAGCGCCATCTTGGCTTAGCCTCA
TCATTAACGGTCAAACGCGATGGGGTGTTGATCCGCAAAGTCAACGGTATCTTAGCCGGGGCAGTACAAGGCAATACCGA
CGGAGTGAAAACGTGGTATCACTTCGATATTCGCCCTGAAATGTGGGTGATGACCCTCAATCAAGACAGCCGTATTTTTC
AACATCAATCGGTGCCGACCATCTTAAAAACCTTATTGGATGAGGCCCATGTTAAAGCGGATTGCCAATTCTACCGTGAA
GATCTACACCCTGAACGTCTCTACACCACCCAAAAACGCGAATCGGCATTTGATTTTTGGTGTCGACTGGCGTTTGAAGA
GGGCATTAACTTCTGGTTTGAAGAAGAACAACTGTTTTACAGCGATGAACATATGGGGATGACGGCGGGTATTCACCTCA
CCTATAACCCGCAGGCCGACACCGATATCAGCGATAGCACTGCCATGACTTGGCAATATGGCGAATATTTGTGTGTGGAT
GAAACTATCCATAAAGACAACAACTTTGTTCGTCCTTCTTATCCGTTGTCTCATGAAAACAAAATTGAGCAGGGGGGACA
GCACAGTGTGTTTGAAAGTTATGGTCGTTTTCAATTAGACGATGAAGGTCGACCTCTGACACAGGTGCGCTTTGAGCAAT
TACGTAACGGCAGCAAAGTGGGGCAAGCGACAACAAACTGTTTTGCGCTGATGCCCGGCAAAATCTTCACCTTATCTCAG
CATCCCCATCAGCCGATGAATGATCGCTGGCAAGTGATCAGCGTCAGCCATCATGGTGTACAGCCACTGGCGGACAATAG
CGGGGGCGAAGGCACACAACTGTCGAATAGCGTCTCCTTTATTCCGGGCACGCAAGAGTGGCGTCCCCCTTATCGTTATA
AACCGACTGCCGACGGCGATGAAGTGGCGACGGTGGTAGGACCGCCGGGGGAAGAAATCTACGTCAATGAATATGGCGCC
GTGAAAGTGCATTTTCATTGGGATCGTCGGGGAAGTGCCGATCACAGTGCTTCATGCTGGGTGCGGGTTGCCATGGGTTG
GAGTGGAAATGGCTATGGTTTTTCTGCGGTACCGCGCATCGGCACACAAGTGCTGGTCAGTTACCTGAATGGGGATATCG
ACAGACCGATGATTACCGGTTGTACCTATGATGGTCGTAACGCACCACCGATTAAGTTCCCAGAGAATAAGACACAAACC
ACTTTCCGTAGCCAAACCCACAAAGGGGAAGGTTTTAATGAACTGCGTTTTGAAGATGCCGGCGGTAAGCAAGAGGTGTT
TCTTCATGCGCAGAAGGATATGAATACCGTAGTCCAAAACGATAAAGGAACGACGGTAGGCGCCAATCATACTGAAACCA
TTATGCAAAACCAAAAGATCTCAGTACATGGTACGCAAACTACGGCGGTTCAAGCGGATCAAAAAAATATCGTGTTTGGT
AATCAACATTCAATCGTGGATGGTGAAGTGTTGATCGCATCAGCCAAAGGTATTCGTTTAATTAGTGGCAACTCGGCACT
TCAATTAAACCCTGACGGGACTATCACCTTAGTGTGTAACAACTTTGATTTTTACGGTCACGGTAGCGGTCGGATTGGTA
CTGGTGAGTTATTAGATTTGAATATGGACGGGGCAGGTCCCGGAAACCTTGAGATGAAACCCGATACTAGCACTATCGCT
CAAGCGAAAGATCAGTTTTTCCCTAAAAAATAA					