SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010090
Component protein type	TssF
T6SS ID (Type)	T6SS00838 (Type i1)
Strain	Proteus mirabilis BB2000
Replicon	chromosome
Sequence	Protein sequence (592 a.a.); Nucleotide sequence (1776 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Gibbs KA, Urbanowski ML, Greenberg EP. Genetic determinants of self identity and social recognition in bacteria. Science. 2008 Jul 11;321(5886):256-9. doi: 10.1126/science.1160033. PMID: 18621670 [3] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BB2000_RS03970
Coordinate (Strand)	934390..936165 (-)
NCBI ID	WP_017628432.1
RefSeq	NC_022000
Uniprot ID	UPI0003015DB8
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.3e-118	5..586

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-591	MSSKSYFTNESEYLNELTEQVAKEKPQLVNLLSHNVKDPDTSRIMDGLSYLSGNLRQQIDRQFPELTNSLANMLWPNYARPVPSMTIVEYHPNYAQCQHATKISAGQPFSATPLYVQSEETNHETLFQCRFSQSRDLWLMPTKISNILQQTTAIEITFELATKQPLANIGLDKLCFYLNGAPFTTNQLYFLLSQHVQSASLVTDVHTLPLTHFSVTPVGFQKQDALLPYPKNSYDGYRILQEFFCFPEAFLFLDIHGLDDIPLAIEAEQFKLVINFDLEIPDGVILYDDSIKLNCTPIVNLFPIDSEAINLTGKSEEYVLSPNYQLTECFDIFSINEVRGLRYPNDAKPYPITYTAFESFYHQNHQTQNHGYYRLKISQNEQLYGYSHRLSFVRQQEPQLQQCEETVSVALNCTNRYIASQLSTRSIDLDSASIPYGALSAKNLFAPTLPLYPLLDKTLYWSELTNLSLNYQSLLNLDSLKKILQLYDLPSVYNTQVARQSQKRLDALVDLHSEHIEHIYKGLPVRGLKTTLVVHQNAFLSEGSLFLFCTVIAQFFTLYTSINMFHELEVINLDNKEVYRWPAQINQHILK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9931	-	-

Protein sequence: 592 a.a.

						>T6CP010090 NC_022000:c936165-934390 [Proteus mirabilis BB2000] [TssF]
MSSKSYFTNESEYLNELTEQVAKEKPQLVNLLSHNVKDPDTSRIMDGLSYLSGNLRQQIDRQFPELTNSLANMLWPNYAR
PVPSMTIVEYHPNYAQCQHATKISAGQPFSATPLYVQSEETNHETLFQCRFSQSRDLWLMPTKISNILQQTTAIEITFEL
ATKQPLANIGLDKLCFYLNGAPFTTNQLYFLLSQHVQSASLVTDVHTLPLTHFSVTPVGFQKQDALLPYPKNSYDGYRIL
QEFFCFPEAFLFLDIHGLDDIPLAIEAEQFKLVINFDLEIPDGVILYDDSIKLNCTPIVNLFPIDSEAINLTGKSEEYVL
SPNYQLTECFDIFSINEVRGLRYPNDAKPYPITYTAFESFYHQNHQTQNHGYYRLKISQNEQLYGYSHRLSFVRQQEPQL
QQCEETVSVALNCTNRYIASQLSTRSIDLDSASIPYGALSAKNLFAPTLPLYPLLDKTLYWSELTNLSLNYQSLLNLDSL
KKILQLYDLPSVYNTQVARQSQKRLDALVDLHSEHIEHIYKGLPVRGLKTTLVVHQNAFLSEGSLFLFCTVIAQFFTLYT
SINMFHELEVINLDNKEVYRWPAQINQHILK*					

Nucleotide sequence: 1776 bp

						>T6CP010090 NC_022000:c936165-934390 [Proteus mirabilis BB2000] [TssF]
ATGTCATCAAAATCCTACTTTACCAATGAATCAGAATACTTAAATGAGCTGACTGAGCAAGTTGCCAAAGAAAAACCGCA
ACTTGTTAATTTGCTTTCGCACAATGTTAAAGATCCTGATACTTCGCGGATTATGGATGGGCTATCTTATTTATCGGGTA
ATTTACGCCAGCAAATAGATAGACAATTTCCTGAGTTAACTAACAGTTTAGCCAATATGCTTTGGCCAAACTACGCGCGC
CCTGTGCCAAGCATGACGATTGTTGAATATCATCCTAATTATGCTCAGTGCCAACACGCAACAAAAATTTCAGCAGGACA
ACCTTTTAGTGCCACTCCACTTTATGTGCAAAGTGAGGAAACAAACCACGAGACGTTATTTCAATGTCGATTTAGTCAAA
GTCGCGACCTCTGGTTAATGCCGACAAAAATCAGTAACATTTTGCAGCAAACAACCGCCATTGAAATAACATTTGAGTTA
GCAACAAAACAGCCACTTGCCAATATTGGCTTAGATAAATTGTGTTTTTACTTAAATGGTGCGCCATTTACTACAAATCA
GCTCTATTTTTTATTAAGCCAGCATGTGCAGAGTGCCTCTTTAGTCACAGATGTACATACGCTACCTCTTACTCACTTTT
CAGTTACGCCTGTTGGTTTTCAAAAGCAGGATGCCTTACTTCCATACCCGAAAAATAGCTATGATGGCTATCGTATACTG
CAAGAGTTTTTCTGTTTTCCTGAAGCCTTTCTCTTTCTAGATATTCATGGTTTGGATGACATTCCTTTGGCTATCGAAGC
AGAGCAATTCAAACTGGTGATCAATTTTGATCTAGAAATTCCTGATGGCGTTATTCTTTACGACGACAGTATTAAACTTA
ATTGCACCCCTATTGTGAATTTATTTCCTATTGATAGTGAAGCAATAAATTTGACGGGGAAAAGTGAGGAGTATGTTTTA
AGTCCTAATTACCAACTTACTGAATGTTTTGATATTTTTTCGATTAATGAGGTTAGAGGGTTACGTTATCCTAATGATGC
TAAACCTTATCCGATCACCTATACAGCATTTGAAAGTTTTTATCATCAAAACCATCAGACTCAAAATCATGGCTATTACC
GTTTAAAGATAAGTCAAAATGAACAGTTATACGGCTATTCCCATCGACTTTCTTTTGTTCGTCAGCAAGAGCCTCAGTTA
CAACAGTGTGAAGAAACCGTTTCTGTTGCACTCAACTGTACTAACCGCTATATCGCTTCCCAGTTATCGACGCGTTCTAT
CGATTTAGATAGTGCATCAATTCCTTATGGGGCTTTATCTGCTAAGAATTTATTCGCACCAACACTGCCTTTATATCCAT
TATTAGATAAAACATTATATTGGTCTGAATTAACTAATTTATCTTTAAATTATCAGTCTTTGCTCAATCTTGATTCATTA
AAAAAAATATTACAACTCTATGATTTACCGTCTGTTTATAACACACAGGTTGCAAGGCAATCACAAAAACGCCTAGATGC
ATTAGTTGATTTACATTCAGAGCATATTGAGCATATCTACAAAGGCCTTCCTGTTCGCGGTCTGAAGACAACGTTAGTTG
TTCATCAAAATGCGTTTCTTTCGGAAGGAAGTTTATTCCTATTTTGTACCGTTATCGCTCAATTTTTCACCTTATATACC
AGCATTAATATGTTTCATGAGTTGGAAGTTATTAACCTTGATAATAAGGAAGTTTATCGATGGCCAGCACAAATCAACCA
GCACATACTGAAGTAA					