SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010089
Component protein type	TssG
T6SS ID (Type)	T6SS00838 (Type i1)
Strain	Proteus mirabilis BB2000
Replicon	chromosome
Sequence	Protein sequence (346 a.a.); Nucleotide sequence (1038 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Gibbs KA, Urbanowski ML, Greenberg EP. Genetic determinants of self identity and social recognition in bacteria. Science. 2008 Jul 11;321(5886):256-9. doi: 10.1126/science.1160033. PMID: 18621670 [3] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BB2000_RS03965
Coordinate (Strand)	933389..934426 (-)
NCBI ID	WP_004244621.1
RefSeq	NC_022000
Uniprot ID	B4ET57_PROMH, A0A1Z1T117_PROMI
KEGG ID	pmr:PMI0745
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	3.4e-87	25..331

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-345	MASTNQPAHTEVIDRWLEQAGSSVTEYNFYQLVELLNKIIHQDVNSMEDDSGILRFKSYSNIAFPTRDVISIEKNNVEQYELEVAFLGLQGSQSPLPGYYLDEFAWEDAQQIEGISAFLDIFNHRLIALLYRIWRKYRYYICFENEGQDSFSQYMFSLVGLGNGANRGRIKINHSKMLAYAGLLASPSRSSDVISSLISHCFDLENVKVIGWETRRVPIPESQQNKLGTISHQSGQKSRPRMLLGENFSLGSHIYDCNGKCTIEISELSLERYMRFLPNGSDFAPLVAFVSYIFHEQLAWDLRLSIAEKQAEGFRLGHQQHNQLGWQSFLGQPAKKPDVTITVLE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9895	-	-

Protein sequence: 346 a.a.

						>T6CP010089 NC_022000:c934426-933389 [Proteus mirabilis BB2000] [TssG]
MASTNQPAHTEVIDRWLEQAGSSVTEYNFYQLVELLNKIIHQDVNSMEDDSGILRFKSYSNIAFPTRDVISIEKNNVEQY
ELEVAFLGLQGSQSPLPGYYLDEFAWEDAQQIEGISAFLDIFNHRLIALLYRIWRKYRYYICFENEGQDSFSQYMFSLVG
LGNGANRGRIKINHSKMLAYAGLLASPSRSSDVISSLISHCFDLENVKVIGWETRRVPIPESQQNKLGTISHQSGQKSRP
RMLLGENFSLGSHIYDCNGKCTIEISELSLERYMRFLPNGSDFAPLVAFVSYIFHEQLAWDLRLSIAEKQAEGFRLGHQQ
HNQLGWQSFLGQPAKKPDVTITVLE*					

Nucleotide sequence: 1038 bp

						>T6CP010089 NC_022000:c934426-933389 [Proteus mirabilis BB2000] [TssG]
ATGGCCAGCACAAATCAACCAGCACATACTGAAGTAATCGATCGTTGGCTGGAACAAGCTGGCTCATCGGTAACAGAGTA
TAATTTTTATCAACTCGTTGAATTGTTAAATAAAATAATTCATCAAGATGTCAACTCGATGGAGGATGACAGCGGTATTC
TTCGCTTTAAATCTTATTCAAATATTGCATTTCCGACGCGAGATGTCATTTCCATTGAAAAAAATAATGTCGAACAATAT
GAATTAGAAGTCGCTTTTTTGGGCTTACAAGGAAGTCAGTCCCCCCTACCAGGCTATTATCTGGATGAGTTTGCTTGGGA
AGATGCACAACAGATAGAAGGTATCTCCGCTTTTCTCGATATCTTCAATCATCGTCTGATAGCGTTACTTTATCGGATCT
GGCGCAAATATCGTTACTACATTTGCTTTGAAAATGAAGGTCAGGATTCATTTTCACAATATATGTTTTCTCTCGTTGGA
TTAGGTAATGGAGCTAATCGCGGTCGGATCAAAATCAACCATAGCAAAATGCTCGCTTATGCTGGTCTTTTGGCGAGTCC
AAGTCGCTCTTCTGATGTGATTAGTAGTCTTATATCTCACTGTTTCGACTTAGAAAATGTAAAAGTAATTGGTTGGGAGA
CACGCCGTGTTCCTATTCCTGAATCACAACAAAACAAGTTAGGTACGATTTCTCATCAATCCGGACAAAAATCTCGCCCA
CGAATGTTATTAGGGGAGAATTTTAGTCTGGGCTCACACATATATGATTGCAATGGAAAATGCACCATCGAAATCAGTGA
ATTAAGTTTAGAACGCTATATGCGTTTTTTACCTAACGGCAGTGATTTCGCACCATTAGTTGCCTTTGTTTCTTACATTT
TTCATGAGCAATTAGCTTGGGATTTACGTCTTAGCATTGCTGAAAAGCAAGCCGAAGGGTTCCGTTTGGGTCACCAACAA
CACAACCAATTAGGCTGGCAAAGCTTTTTAGGTCAACCCGCAAAGAAACCGGATGTCACCATTACGGTATTGGAATAA					