SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP010088
Component protein type	TssJ
T6SS ID (Type)	T6SS00838 (Type i1)
Strain	Proteus mirabilis BB2000
Replicon	chromosome
Sequence	Protein sequence (184 a.a.); Nucleotide sequence (552 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Gibbs KA, Urbanowski ML, Greenberg EP. Genetic determinants of self identity and social recognition in bacteria. Science. 2008 Jul 11;321(5886):256-9. doi: 10.1126/science.1160033. PMID: 18621670 [3] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BB2000_RS03955
Coordinate (Strand)	931563..932114 (-)
NCBI ID	WP_004244619.1
RefSeq	NC_022000
Uniprot ID	B4ET55_PROMH, A0A1Z1SXK8_PROMI
KEGG ID	pvl:AOB99_06105, pmr:PMI0743
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.3e-26	51..173

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-183	MQLRTFSILLLFSSFLILSGCSKPFEAAGKMGKILLDPSTPVGQPKDQPSTVALTILAEPYINPNQEGEAAPINIQVVYLNEDSVFGSVDHDQIDAKEGDLEKVLKKNYIDHQDYTILPGQYKPFPTMTLDPSNRYIGIIAYYSDPDKAEWKKIVKARGKGRHYFLLVHIRENEIEFRQEEDD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9992	pos: 20-21. LSG-CS.	MQLRTFSILLLFSSFLILSG

Protein sequence: 184 a.a.

						>T6CP010088 NC_022000:c932114-931563 [Proteus mirabilis BB2000] [TssJ]
MQLRTFSILLLFSSFLILSGCSKPFEAAGKMGKILLDPSTPVGQPKDQPSTVALTILAEPYINPNQEGEAAPINIQVVYL
NEDSVFGSVDHDQIDAKEGDLEKVLKKNYIDHQDYTILPGQYKPFPTMTLDPSNRYIGIIAYYSDPDKAEWKKIVKARGK
GRHYFLLVHIRENEIEFRQEEDD*					

Nucleotide sequence: 552 bp

						>T6CP010088 NC_022000:c932114-931563 [Proteus mirabilis BB2000] [TssJ]
ATGCAGCTTAGAACTTTCTCAATATTACTGTTATTTAGCTCTTTTTTAATATTGAGTGGATGTAGTAAACCTTTTGAAGC
CGCGGGTAAAATGGGGAAAATCTTATTAGATCCCTCAACTCCTGTTGGGCAACCGAAAGATCAGCCCTCTACCGTTGCAT
TAACCATCCTTGCGGAGCCCTATATTAATCCTAATCAGGAAGGTGAAGCTGCGCCGATAAATATCCAAGTTGTTTATCTT
AATGAAGATTCAGTATTTGGCTCTGTTGATCATGATCAGATAGATGCAAAAGAGGGTGACTTAGAGAAAGTTCTAAAGAA
AAATTATATTGACCATCAAGACTACACCATTTTACCTGGCCAATATAAACCCTTCCCTACTATGACGTTAGACCCATCTA
ACCGTTACATTGGCATTATTGCCTATTACTCAGATCCTGATAAAGCAGAATGGAAGAAAATAGTCAAAGCGCGTGGTAAA
GGACGCCACTACTTCCTATTAGTGCACATTAGGGAAAATGAAATCGAATTTAGACAAGAAGAGGATGATTAA					