Detailed information of component protein


Summary

Component ID T6CP010082
Component protein type TssA
T6SS ID (Type) T6SS00838 experimental (Type i1)
Strain Proteus mirabilis BB2000
Replicon chromosome
Sequence Protein sequence (452 a.a.); Nucleotide sequence (1356 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BB2000_RS03915
Coordinate (Strand) 918878..920233 (-)
NCBI ID WP_020945426.1
RefSeq NC_022000
Uniprot ID UPI0003841C8A
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 3.8e-35 287..430
ImpA_N PF06812.14 2.5e-19 15..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-234 MLSAMKSESLVIKIGGSPLDTPEFIALKTEFNKLSHPARPEISWTLIESLCLSLFKNHGIDLQSGAYYTIARLQQHGLSGFTEGCELLANVIVTYWDSLWPEKPNQRTEVLNWFNTKASALLRQQSYQAKDLRLIYRSERALQLIIDKLAKTTWEKLPKIENLLWFFQNLAKQIEKQEKQTQQDSAEPVSVPPLVCIQQPSKTTDKAAPTFVIETPQPASSFTVVAKKMSATRG
Transmembrane helix 235-257 FIWGCVLSCILFLAVGFGVHLYL
Outside 258-451 KQEMNAITSIPEGAAAKWLFKPQLNSYEYHLALLEKRSPLSNLRLIDTMQEKAKNAWPTDAEQIYFTRNWQNLYQTRLENMPINDSWSETATLLQQLSNKIVQQERNRGSFTLSYLKTAIYDIQKQHNKVEPIEEKLRQLAVQIETGQPISPATFNNIDNKINGLLARYYDLQKQAEQQGLKPGSYSSFGLDHE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9916 - -


  Protein sequence: 452 a.a.    

>T6CP010082 NC_022000:c920233-918878 [Proteus mirabilis BB2000] [TssA]
MLSAMKSESLVIKIGGSPLDTPEFIALKTEFNKLSHPARPEISWTLIESLCLSLFKNHGIDLQSGAYYTIARLQQHGLSG
FTEGCELLANVIVTYWDSLWPEKPNQRTEVLNWFNTKASALLRQQSYQAKDLRLIYRSERALQLIIDKLAKTTWEKLPKI
ENLLWFFQNLAKQIEKQEKQTQQDSAEPVSVPPLVCIQQPSKTTDKAAPTFVIETPQPASSFTVVAKKMSATRGFIWGCV
LSCILFLAVGFGVHLYLKQEMNAITSIPEGAAAKWLFKPQLNSYEYHLALLEKRSPLSNLRLIDTMQEKAKNAWPTDAEQ
IYFTRNWQNLYQTRLENMPINDSWSETATLLQQLSNKIVQQERNRGSFTLSYLKTAIYDIQKQHNKVEPIEEKLRQLAVQ
IETGQPISPATFNNIDNKINGLLARYYDLQKQAEQQGLKPGSYSSFGLDHE*

  Nucleotide sequence: 1356 bp    

>T6CP010082 NC_022000:c920233-918878 [Proteus mirabilis BB2000] [TssA]
ATGTTGAGTGCGATGAAATCAGAAAGTTTAGTGATTAAAATTGGGGGATCTCCACTTGATACCCCTGAATTTATTGCCCT
GAAAACCGAATTTAATAAATTGAGCCACCCTGCTCGACCAGAAATAAGTTGGACACTTATTGAGTCACTTTGCTTATCCC
TATTTAAAAATCATGGTATCGACCTACAAAGCGGTGCTTACTATACCATTGCAAGATTACAACAGCATGGTTTAAGCGGT
TTTACTGAAGGATGTGAGTTATTAGCAAATGTTATTGTGACTTATTGGGATAGCCTGTGGCCGGAAAAGCCAAACCAACG
TACAGAGGTATTAAACTGGTTTAATACCAAAGCCAGCGCTTTATTAAGACAACAATCCTATCAAGCAAAAGATCTACGAT
TAATCTATCGTTCAGAGCGCGCGTTACAGTTAATAATCGATAAACTGGCTAAAACAACTTGGGAAAAACTGCCTAAAATT
GAAAACTTACTGTGGTTTTTCCAAAACTTAGCCAAACAAATTGAAAAACAAGAAAAACAGACGCAACAAGATAGTGCCGA
ACCCGTTAGCGTACCGCCATTAGTCTGTATTCAACAACCGTCGAAAACAACGGACAAAGCAGCCCCCACTTTTGTGATTG
AAACGCCTCAACCCGCCTCTTCTTTCACTGTAGTAGCAAAAAAAATGAGTGCGACCCGAGGTTTTATATGGGGCTGTGTG
CTCAGTTGCATACTGTTTTTAGCTGTCGGATTTGGTGTACATCTCTATTTAAAACAAGAGATGAATGCGATTACTTCAAT
ACCTGAAGGTGCGGCTGCTAAATGGTTGTTTAAACCACAGTTAAATAGCTACGAATATCATTTAGCATTATTAGAAAAAC
GTTCTCCCCTTTCTAATTTACGGCTAATTGATACGATGCAAGAAAAAGCCAAAAACGCTTGGCCTACAGATGCAGAGCAA
ATTTATTTTACACGTAATTGGCAAAATCTTTATCAAACTCGCTTAGAGAATATGCCAATTAATGATAGCTGGTCTGAAAC
CGCGACATTATTACAACAACTTTCTAATAAAATAGTTCAGCAAGAACGAAATAGAGGCAGTTTTACACTCTCTTATCTAA
AAACGGCAATTTATGATATTCAAAAACAGCACAATAAAGTTGAGCCAATCGAAGAAAAACTGCGACAGCTCGCTGTACAA
ATTGAAACAGGGCAACCTATTTCTCCTGCAACTTTTAATAATATTGATAATAAAATTAATGGGTTATTAGCTCGTTATTA
TGATTTACAAAAACAAGCTGAACAACAAGGTTTAAAACCAGGCTCATATTCATCTTTTGGATTAGATCATGAGTGA