SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009656
Component protein type	TssE
T6SS ID (Type)	T6SS00431 (Type i4a)
Strain	Pseudomonas brassicacearum subsp. brassicacearum NFM421
Replicon	chromosome
Sequence	Protein sequence (148 a.a.); Nucleotide sequence (444 bp)
Reference	[1] Ortet P, Barakat M, Lalaouna D, et al. Complete genome sequence of a beneficial plant root-associated bacterium, Pseudomonas brassicacearum. J Bacteriol. 2011 Jun;193(12):3146. doi: 10.1128/JB.00411-11. Epub 2011 Apr 22. PMID: 21515771 [2] Lalaouna D, Fochesato S, Sanchez L, et al. Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs. Appl Environ Microbiol. 2012 Mar;78(6):1658-65. doi: 10.1128/AEM.06769-11. Epub 2012 Jan 13. PMID: 22247157

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PSEBR_RS17640
Coordinate (Strand)	4103417..4103860 (+)
NCBI ID	WP_003202805.1
RefSeq	NC_015379
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	1e-16	29..120

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 148 a.a.

						>T6CP009656 NC_015379:4103417-4103860 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssE]
MAGFGLRPPLFERLASPANDTGRAFDRQALQDSVHAELSRLFNTRRGPRALTEPPSILDYGIADWTALQQQRSDDRRQLT
REIRQAITHFEPRLRLGEVEVCPVPGHPQQLCIRLLGELRGDLHAWPVAFVIEHAGDGLEVRHERLD*					

Nucleotide sequence: 444 bp

						>T6CP009656 NC_015379:4103417-4103860 [Pseudomonas brassicacearum subsp. brassicacearum NFM421] [TssE]
ATGGCTGGCTTCGGGCTGCGCCCGCCCCTGTTCGAACGCCTGGCCAGCCCGGCGAACGATACCGGGCGGGCGTTCGACCG
ACAGGCGTTGCAAGACTCGGTGCATGCCGAACTGTCACGCCTGTTCAATACCCGACGCGGCCCACGGGCGCTGACCGAAC
CGCCGAGCATCCTGGACTACGGCATCGCCGACTGGACCGCGCTGCAACAGCAGCGCAGCGATGACCGGCGTCAACTGACC
CGGGAAATCCGCCAGGCCATCACCCACTTCGAACCTCGCCTGCGCCTGGGCGAGGTCGAGGTCTGCCCGGTGCCGGGCCA
TCCGCAGCAACTGTGCATACGGCTGTTGGGTGAGCTGCGCGGCGACCTGCACGCATGGCCCGTGGCCTTTGTCATCGAGC
ACGCCGGCGACGGCCTTGAGGTACGACATGAGCGACTCGATTGA					