Detailed information of component protein


Summary

Component ID T6CP009580
Component protein type TssM
T6SS ID (Type) T6SS00013 experimental (Type i1)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (1207 a.a.); Nucleotide sequence (3621 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PP_RS13695
Coordinate (Strand) 3003420..3007040 (+)
NCBI ID WP_010953547.1
RefSeq NC_002947
Uniprot ID Q88JM2_PSEPK, A0A2V4FA92_9PSED
KEGG ID ppu:PP_2627
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 2.8e-68 525..830
IcmF-related_N PF14331.8 1.7e-67 219..473
IcmF_C PF06744.14 2.3e-22 1064..1168
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-18 MNQIWTQLKRWGWPWVTR
Transmembrane helix 19-41 IGLALPLLLGLGALLMLVAIWWL
Outside 42-55 GPQWTWREQQPLAS
Transmembrane helix 56-78 VAHRSVASLVLVLVPLLCWLGVL
Inside 79-467 RTRFRRLQAERRQALAAEQDVALPFVQAQERALSQGLERYLDNAGGRRALYRLPWYLVLGDEQSGKSSFIDRTDQRFTLTRIDQAQARGRQTQAMAYPVGWWVSDDAVIIDPPGVFISQKPPVGSAANAPSEASAPPGIQARLWEHLLGWLQRKRSQRALNGVLLVVDLPALLHGRPEQRVALAHGLRTRLYEVSSQLGARLPLYVVLTKFDLLDGFDQFYSQLPAAKRKSLLGFTFKLDAVDAFDAWLDEYDEHYGRLLTQLQEQVIDRLDVLGKTAPCGRLFSLHAQLIGLRPILQAFLRETLASDRFTTPPLVRGVYWSSVVQQGDMRNAFVREAAQPYTTKLPLREGKAQGKALVYFIQQAFRRVIYQEAGLAGDNVRVARSKRH
Transmembrane helix 468-487 LLWVGSGVGILAFSIAVASW
Outside 488-1206 QRYADINGAKAASVLAKSQEYSGHEVDMRLDPTGRNLLAPLDQIRDAVAVFGDYRAAWPVVADFGLYQGRNIGPLVDEAYLSLLSKRFLPALASGVIDAMNAAPPGSEQQMAALRVYRMIEDRNSRRPEWVEDWMARQWQRAFPGQGQLQRDLMQHLKYALAYADTDLPQYRQRVSEVQQALRKLPLPQRVYAGLKQQSYQQLHAGLDLRHQVGPAFDVVYQPSSGARQGDEDVRLAAMLTAKGFREYFEPHSQRFAEMAMVDQWALGERSQLDYSDVDRDALTERLYNLYSADYIDSWRRALTAFTVADFRDLDHGVAILEQLTGPAAPLQRLLDTVRDNTSLAPPAAVEASGELSTLRVATGKPEQQQALAIQRAFAGLGAMLQATGEKPSYYDETLGAVAAVLDYAKAVQDSPDRGKAALQAVHQRFAMTGQDPIGTLQRIATGLPEPISHQVRKLADQTAQVLNVEALRELERRWDADVYSFFQQRLAGRYPFVVKAPDASLDDFEAFFGPKGRLQQFNDRYLKVFLKDNLEALQSAQHGQSLIRADVIEQLELAERIRETFFDQRGKLSVQFSIEPLGLSANQRTSLLDLDGQLIAYTHGPSHITGIVWPNTLGQQVRSNLTLLRQNGNSSSLEYRGPWSMFRLLSRGALNGRTATSVDLSFRTGDGVMRYRLNAEKAFNPITQQPFKGFRLPRGLLQQPASVVQAVLPDGQRL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9959 - -


  Protein sequence: 1207 a.a.    

>T6CP009580 NC_002947:3003420-3007040 [Pseudomonas putida KT2440] [TssM]
MNQIWTQLKRWGWPWVTRIGLALPLLLGLGALLMLVAIWWLGPQWTWREQQPLASVAHRSVASLVLVLVPLLCWLGVLRT
RFRRLQAERRQALAAEQDVALPFVQAQERALSQGLERYLDNAGGRRALYRLPWYLVLGDEQSGKSSFIDRTDQRFTLTRI
DQAQARGRQTQAMAYPVGWWVSDDAVIIDPPGVFISQKPPVGSAANAPSEASAPPGIQARLWEHLLGWLQRKRSQRALNG
VLLVVDLPALLHGRPEQRVALAHGLRTRLYEVSSQLGARLPLYVVLTKFDLLDGFDQFYSQLPAAKRKSLLGFTFKLDAV
DAFDAWLDEYDEHYGRLLTQLQEQVIDRLDVLGKTAPCGRLFSLHAQLIGLRPILQAFLRETLASDRFTTPPLVRGVYWS
SVVQQGDMRNAFVREAAQPYTTKLPLREGKAQGKALVYFIQQAFRRVIYQEAGLAGDNVRVARSKRHLLWVGSGVGILAF
SIAVASWQRYADINGAKAASVLAKSQEYSGHEVDMRLDPTGRNLLAPLDQIRDAVAVFGDYRAAWPVVADFGLYQGRNIG
PLVDEAYLSLLSKRFLPALASGVIDAMNAAPPGSEQQMAALRVYRMIEDRNSRRPEWVEDWMARQWQRAFPGQGQLQRDL
MQHLKYALAYADTDLPQYRQRVSEVQQALRKLPLPQRVYAGLKQQSYQQLHAGLDLRHQVGPAFDVVYQPSSGARQGDED
VRLAAMLTAKGFREYFEPHSQRFAEMAMVDQWALGERSQLDYSDVDRDALTERLYNLYSADYIDSWRRALTAFTVADFRD
LDHGVAILEQLTGPAAPLQRLLDTVRDNTSLAPPAAVEASGELSTLRVATGKPEQQQALAIQRAFAGLGAMLQATGEKPS
YYDETLGAVAAVLDYAKAVQDSPDRGKAALQAVHQRFAMTGQDPIGTLQRIATGLPEPISHQVRKLADQTAQVLNVEALR
ELERRWDADVYSFFQQRLAGRYPFVVKAPDASLDDFEAFFGPKGRLQQFNDRYLKVFLKDNLEALQSAQHGQSLIRADVI
EQLELAERIRETFFDQRGKLSVQFSIEPLGLSANQRTSLLDLDGQLIAYTHGPSHITGIVWPNTLGQQVRSNLTLLRQNG
NSSSLEYRGPWSMFRLLSRGALNGRTATSVDLSFRTGDGVMRYRLNAEKAFNPITQQPFKGFRLPRGLLQQPASVVQAVL
PDGQRL*

  Nucleotide sequence: 3621 bp    

>T6CP009580 NC_002947:3003420-3007040 [Pseudomonas putida KT2440] [TssM]
ATGAATCAAATATGGACGCAACTCAAACGTTGGGGCTGGCCGTGGGTAACGCGCATTGGCCTGGCGCTGCCGCTGCTGCT
CGGGCTTGGCGCCTTGCTGATGCTGGTCGCCATCTGGTGGCTTGGCCCGCAATGGACGTGGCGTGAGCAGCAGCCGCTGG
CAAGTGTGGCTCATCGCAGCGTGGCAAGCCTGGTGCTGGTGCTGGTGCCGCTTCTGTGCTGGCTTGGGGTGCTGCGCACG
CGCTTTCGCCGCCTGCAAGCGGAGCGCCGCCAGGCACTGGCCGCCGAACAGGATGTCGCGCTACCATTCGTGCAGGCGCA
AGAGCGGGCCTTGAGCCAGGGGCTTGAGCGGTACCTCGACAATGCCGGTGGGCGACGCGCGTTGTACCGGCTGCCCTGGT
ACCTGGTGTTGGGCGACGAACAGTCCGGTAAAAGCAGCTTCATCGACCGCACCGATCAGCGCTTTACCCTGACGCGTATC
GACCAGGCCCAGGCACGGGGCCGACAGACTCAAGCAATGGCCTACCCGGTAGGCTGGTGGGTCAGCGACGATGCCGTGAT
CATCGACCCGCCCGGTGTTTTCATCAGTCAAAAACCACCGGTGGGCTCGGCGGCCAACGCCCCCTCCGAAGCCTCCGCGC
CGCCCGGCATCCAGGCCCGGTTATGGGAGCACCTGTTGGGCTGGCTGCAGCGTAAGCGAAGCCAGCGGGCGCTCAATGGA
GTGTTGCTGGTGGTCGACCTGCCGGCGTTGCTGCATGGCAGGCCCGAGCAGCGCGTCGCGCTGGCCCATGGGTTGCGCAC
ACGCCTGTACGAAGTGAGCAGTCAGTTGGGGGCGCGCCTGCCGCTGTACGTGGTACTGACCAAATTCGATCTGCTGGACG
GCTTCGACCAGTTCTACAGCCAGCTTCCGGCCGCCAAGCGTAAAAGCCTGTTGGGGTTCACCTTCAAGCTGGATGCGGTT
GACGCGTTCGACGCCTGGCTGGATGAGTATGACGAGCACTACGGGCGGCTTCTCACCCAATTGCAGGAGCAGGTCATCGA
CCGGCTGGATGTACTGGGCAAGACAGCGCCGTGCGGGCGCCTGTTCTCACTGCATGCACAGTTGATCGGGTTGCGCCCCA
TACTGCAGGCATTCCTGCGCGAAACCTTGGCGAGTGACCGGTTCACCACGCCACCCTTGGTACGTGGTGTGTACTGGTCG
TCAGTGGTACAGCAAGGCGACATGCGCAATGCCTTTGTCCGAGAAGCGGCTCAGCCCTACACGACGAAGCTGCCCCTGCG
TGAAGGTAAGGCACAGGGGAAGGCGTTGGTCTATTTCATCCAGCAAGCATTCAGGCGGGTTATCTATCAGGAAGCCGGGC
TGGCGGGTGACAATGTTCGGGTTGCGCGCAGCAAGCGCCACTTGCTGTGGGTGGGCTCTGGTGTAGGCATCCTGGCGTTC
AGTATCGCCGTTGCCAGCTGGCAGCGTTACGCCGACATCAATGGCGCCAAGGCTGCCAGTGTGCTCGCCAAAAGCCAGGA
GTACAGCGGTCACGAAGTCGATATGCGTCTTGATCCGACCGGGCGCAACCTGCTGGCGCCACTGGACCAGATTCGCGATG
CGGTCGCGGTGTTTGGCGACTATCGTGCGGCCTGGCCGGTGGTTGCCGATTTTGGCTTGTATCAAGGGCGCAACATTGGT
CCCTTGGTTGATGAGGCGTACCTCAGCTTGTTGTCTAAACGTTTCTTGCCGGCCTTGGCCAGTGGTGTGATCGATGCCAT
GAACGCAGCACCACCTGGCAGCGAGCAACAAATGGCCGCGCTCAGGGTTTACCGGATGATCGAGGACCGCAACAGCCGCC
GGCCCGAATGGGTCGAGGACTGGATGGCCCGTCAGTGGCAACGGGCCTTTCCTGGCCAGGGGCAGTTGCAGCGTGACCTC
ATGCAGCACCTGAAATACGCCTTGGCCTATGCCGATACCGATTTGCCGCAGTACCGCCAGCGGGTCAGCGAAGTGCAGCA
GGCGCTGCGCAAATTGCCGTTGCCACAGCGGGTCTATGCAGGGCTGAAACAACAATCGTACCAGCAGTTGCACGCCGGGC
TGGACCTGCGCCACCAGGTAGGGCCTGCGTTCGATGTGGTGTACCAGCCGTCTTCCGGGGCCAGGCAAGGGGATGAGGAT
GTACGGCTGGCCGCCATGCTCACCGCCAAAGGCTTCAGGGAATACTTCGAGCCGCACAGCCAACGCTTCGCTGAAATGGC
GATGGTTGATCAATGGGCATTGGGGGAGCGCAGTCAACTCGACTATTCGGACGTCGACCGCGATGCGCTGACCGAGCGGC
TGTATAACCTCTACAGCGCCGATTACATTGACAGCTGGCGGCGCGCCCTGACCGCTTTTACGGTGGCTGACTTCCGTGAT
CTGGACCATGGTGTCGCGATCCTGGAGCAGTTGACCGGCCCCGCAGCACCATTGCAACGGCTGCTGGATACGGTCAGGGA
TAACACCTCGCTTGCGCCTCCAGCAGCTGTTGAAGCATCGGGTGAGCTGAGTACGCTGCGCGTAGCAACCGGAAAACCCG
AGCAACAGCAAGCGTTGGCCATCCAGCGGGCGTTTGCAGGCCTTGGCGCCATGCTGCAGGCGACAGGAGAAAAGCCGAGC
TACTACGATGAAACCCTTGGCGCTGTTGCGGCCGTGCTGGACTACGCCAAGGCCGTACAGGACAGCCCGGACCGCGGCAA
GGCTGCTTTGCAGGCCGTGCATCAGCGCTTTGCGATGACAGGGCAGGACCCGATAGGCACCCTCCAACGTATTGCCACAG
GTTTACCGGAGCCTATCAGCCACCAGGTCAGAAAGCTCGCCGACCAGACTGCCCAGGTGCTGAACGTTGAAGCACTGCGC
GAACTGGAGCGACGTTGGGATGCTGACGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAA
GGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATC
TCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATC
GAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGA
GCCCTTGGGGCTCAGTGCAAACCAGCGTACCAGCCTGCTGGACCTGGATGGCCAGTTGATTGCCTACACCCATGGGCCAA
GCCATATCACGGGTATTGTCTGGCCCAATACCTTGGGGCAGCAGGTCCGCAGCAATCTCACCTTGCTAAGGCAAAATGGC
AACAGCAGCAGCCTGGAGTACCGTGGCCCGTGGTCGATGTTCCGCTTGCTCAGCCGCGGTGCGCTCAATGGCCGGACGGC
GACCAGCGTGGACCTGAGCTTCAGGACCGGAGATGGCGTGATGCGCTATCGGCTTAACGCTGAAAAAGCCTTCAATCCCA
TTACCCAGCAACCTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTA
CCGGATGGGCAGCGACTATGA