SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009576
Component protein type	TssL
T6SS ID (Type)	T6SS00013 (Type i1)
Strain	Pseudomonas putida KT2440
Replicon	chromosome
Sequence	Protein sequence (290 a.a.); Nucleotide sequence (870 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [3] Bernal P, Allsopp LP, Filloux A, et al. The Pseudomonas putida T6SS is a plant warden against phytopathogens. ISME J. 2017 Apr;11(4):972-987. doi: 10.1038/ismej.2016.169. Epub 2017 Jan 3. PMID: 28045455

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PP_RS13640
Coordinate (Strand)	2992203..2993072 (-)
NCBI ID	WP_010953536.1
RefSeq	NC_002947
Uniprot ID	Q88JN3_PSEPK, A0A2V4FA83_9PSED
KEGG ID	ppu:PP_2616
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	5.5e-62	69..270

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-247	MGKDNNTPTDENNAAVNALDSTLQQPVPAPRAAATNGQASTDKPEPEPVSEGYPADPEFQLRGGFANLMLDAASPLFGLVIRLRTLDELPNIQDVHQQVRSQIDNIQEEMRQHGYEPAQLLAYSYGLCLFIDEAVMERPWGKSSCWSQEPLLSIFHDETWGGEKIFTVITRLMQEPKRYQDVLEFMYFALCLGLKGKYALAPKGEETLNALIHQLHGIIRELRGPTPEEVCDPYTNVAPRNFRMSRQ
Transmembrane helix	248-270	WPWWSPLVISAIAMAVAYGIYSY
Inside	271-289	RLHLITTEVLESLNGILQQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8448	-	-

Protein sequence: 290 a.a.

						>T6CP009576 NC_002947:c2993072-2992203 [Pseudomonas putida KT2440] [TssL]
MGKDNNTPTDENNAAVNALDSTLQQPVPAPRAAATNGQASTDKPEPEPVSEGYPADPEFQLRGGFANLMLDAASPLFGLV
IRLRTLDELPNIQDVHQQVRSQIDNIQEEMRQHGYEPAQLLAYSYGLCLFIDEAVMERPWGKSSCWSQEPLLSIFHDETW
GGEKIFTVITRLMQEPKRYQDVLEFMYFALCLGLKGKYALAPKGEETLNALIHQLHGIIRELRGPTPEEVCDPYTNVAPR
NFRMSRQWPWWSPLVISAIAMAVAYGIYSYRLHLITTEVLESLNGILQQ*					

Nucleotide sequence: 870 bp

						>T6CP009576 NC_002947:c2993072-2992203 [Pseudomonas putida KT2440] [TssL]
ATGGGAAAAGACAACAATACGCCCACCGATGAAAACAACGCTGCAGTCAACGCGCTTGATTCAACCCTTCAACAGCCAGT
ACCCGCCCCCCGGGCTGCAGCAACCAATGGGCAAGCATCCACGGACAAACCTGAGCCAGAGCCCGTTTCCGAAGGCTACC
CCGCTGACCCGGAATTTCAGCTGCGGGGCGGCTTCGCCAACCTGATGCTGGATGCCGCATCACCGTTGTTCGGCTTGGTC
ATACGCCTGCGTACGCTGGACGAGCTGCCGAACATCCAGGACGTGCACCAGCAAGTACGCAGCCAGATCGACAACATTCA
GGAAGAAATGCGCCAGCACGGTTACGAGCCGGCTCAGTTGCTGGCGTACTCCTACGGTCTGTGCCTGTTCATAGACGAGG
CCGTTATGGAGCGGCCCTGGGGCAAGAGCAGCTGCTGGAGCCAGGAGCCGCTGCTCAGCATCTTCCACGACGAGACCTGG
GGTGGGGAAAAAATCTTCACCGTGATTACCCGCCTGATGCAGGAGCCCAAGCGTTATCAGGACGTACTGGAGTTCATGTA
CTTCGCCCTGTGCCTTGGGCTGAAGGGCAAGTACGCGCTCGCGCCGAAAGGCGAAGAAACACTCAACGCATTGATTCACC
AACTGCACGGGATCATCCGCGAACTGCGCGGCCCTACCCCCGAAGAAGTCTGCGACCCCTACACCAATGTGGCCCCACGC
AACTTCCGCATGAGCCGGCAATGGCCCTGGTGGAGCCCCTTGGTGATTTCCGCCATCGCCATGGCGGTGGCCTACGGGAT
CTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAG					