SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009574
Component protein type	TssI
T6SS ID (Type)	T6SS00013 (Type i1)
Strain	Pseudomonas putida KT2440
Replicon	chromosome
Sequence	Protein sequence (723 a.a.); Nucleotide sequence (2169 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [3] Bernal P, Allsopp LP, Filloux A, et al. The Pseudomonas putida T6SS is a plant warden against phytopathogens. ISME J. 2017 Apr;11(4):972-987. doi: 10.1038/ismej.2016.169. Epub 2017 Jan 3. PMID: 28045455

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PP_RS13630
Coordinate (Strand)	2989311..2991479 (-)
NCBI ID	WP_010953534.1
RefSeq	NC_002947
Uniprot ID	Q88JN5_PSEPK, A0A2V4FFC8_9PSED
KEGG ID	ppu:PP_2614
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	1.3e-65	28..349
Phage_base_V	PF04717.14	1.5e-12	408..475

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-722	MPSQSDLRYSFEALVGKAAFEVVSFELREGISAPFELELKLISFENDIDFGHLLDKPVLFTILDGERPVRYVHGLVSSFSQGESGFYRTYYHALVEPQLARARLRSNWRIFQHKTVPQILELMLKRQGIDQYELRASMDHQVREFCVQAGETDLAFIARLAAEEGFVYRFAHSEKLHKLIITDRLQSLGLISHGAIKADHEDEGFGDDDEPVGPDSVLYQANSGGDQAIPCLRRLRYSEQVRTARQVQRDHTFTHPAYRQEHRAAGPFLEHQSKDYEYFDYPGRYKRDAVGKPFTENRITALRHDVRIAEVQGDDVRLQPGLSFTLTDHPREDLNVHWRVSTVHHEGSQPTSLQEEAAGADQGTRYEQKAVLVPGRTEWRPAPLPKPRVDGPHMATVVGPPGEEIYCDEWGRVKVSFPWDRESQNNEFSSCWLRVSQGWAGGSWGSMAIPRIGQDVIIHYVNGDPDQPMITGRTYCGNQLPPYDLPRHKTRMTIKSQTHKGDGFNELRFEDELGQEEVFIHAQRDQNNVVKHDETTQVGNDRKEQVDNDESILIGHDRTETVGNDERITIGQDAALDIGRNHTIRIAKDRVENIGNHRHDQIAANHHTRIGGNFEQRVEGHAELEARGDIRRRTRRYELRASEAVVIQGPGGSIRIDETGITLDSPTIRITGQMLKSANGTTSPFSISSAPNTGKPLERLCGRRPDGSCPLPDCRCIRGQVQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9914	-	-

Protein sequence: 723 a.a.

						>T6CP009574 NC_002947:c2991479-2989311 [Pseudomonas putida KT2440] [TssI]
MPSQSDLRYSFEALVGKAAFEVVSFELREGISAPFELELKLISFENDIDFGHLLDKPVLFTILDGERPVRYVHGLVSSFS
QGESGFYRTYYHALVEPQLARARLRSNWRIFQHKTVPQILELMLKRQGIDQYELRASMDHQVREFCVQAGETDLAFIARL
AAEEGFVYRFAHSEKLHKLIITDRLQSLGLISHGAIKADHEDEGFGDDDEPVGPDSVLYQANSGGDQAIPCLRRLRYSEQ
VRTARQVQRDHTFTHPAYRQEHRAAGPFLEHQSKDYEYFDYPGRYKRDAVGKPFTENRITALRHDVRIAEVQGDDVRLQP
GLSFTLTDHPREDLNVHWRVSTVHHEGSQPTSLQEEAAGADQGTRYEQKAVLVPGRTEWRPAPLPKPRVDGPHMATVVGP
PGEEIYCDEWGRVKVSFPWDRESQNNEFSSCWLRVSQGWAGGSWGSMAIPRIGQDVIIHYVNGDPDQPMITGRTYCGNQL
PPYDLPRHKTRMTIKSQTHKGDGFNELRFEDELGQEEVFIHAQRDQNNVVKHDETTQVGNDRKEQVDNDESILIGHDRTE
TVGNDERITIGQDAALDIGRNHTIRIAKDRVENIGNHRHDQIAANHHTRIGGNFEQRVEGHAELEARGDIRRRTRRYELR
ASEAVVIQGPGGSIRIDETGITLDSPTIRITGQMLKSANGTTSPFSISSAPNTGKPLERLCGRRPDGSCPLPDCRCIRGQ
VQ*					

Nucleotide sequence: 2169 bp

						>T6CP009574 NC_002947:c2991479-2989311 [Pseudomonas putida KT2440] [TssI]
ATGCCTAGCCAATCTGATTTGCGCTACAGCTTTGAGGCACTCGTTGGCAAAGCTGCATTCGAAGTGGTGTCGTTCGAACT
CCGCGAGGGCATCAGCGCACCCTTTGAACTGGAGCTGAAACTGATCAGCTTCGAAAACGACATCGACTTCGGCCATTTGC
TCGACAAACCCGTGCTCTTCACCATCTTGGACGGAGAACGCCCGGTACGGTACGTGCACGGCCTTGTCAGCAGCTTCAGC
CAGGGCGAGAGCGGCTTCTACCGCACTTACTATCACGCCTTGGTCGAACCGCAACTGGCCCGCGCAAGGCTGCGCTCGAA
CTGGCGCATCTTCCAGCACAAGACCGTTCCGCAAATTCTCGAGCTGATGCTCAAGCGTCAAGGCATCGACCAGTACGAAC
TACGCGCCAGCATGGACCATCAGGTGCGCGAATTCTGCGTTCAGGCCGGTGAGACGGACTTGGCGTTCATCGCCCGCCTT
GCCGCTGAAGAAGGCTTCGTCTACCGCTTTGCGCACAGTGAAAAGCTGCACAAGCTGATCATCACCGATCGGCTGCAGTC
CCTCGGCCTGATCAGCCATGGCGCCATCAAGGCCGACCATGAAGACGAGGGTTTCGGCGATGACGACGAGCCTGTAGGCC
CTGACAGCGTCCTGTACCAAGCGAACAGCGGTGGCGATCAAGCCATACCCTGCCTTCGCCGCTTGCGCTACAGCGAGCAG
GTGCGCACCGCTCGCCAGGTGCAGCGCGACCACACCTTCACCCACCCGGCCTACCGCCAGGAGCACAGAGCCGCCGGGCC
CTTCCTGGAGCATCAGAGCAAAGATTACGAATACTTCGATTACCCAGGCCGCTATAAACGCGATGCCGTGGGCAAGCCGT
TCACCGAAAACCGCATCACAGCGCTGCGCCATGATGTGCGTATTGCCGAGGTCCAGGGCGATGACGTGCGCCTGCAACCG
GGGCTCAGTTTCACCCTGACCGACCACCCGCGCGAAGACCTGAATGTGCATTGGCGGGTCAGCACGGTCCACCACGAAGG
CAGCCAGCCCACCAGCCTGCAGGAAGAAGCTGCCGGGGCCGACCAGGGCACACGTTACGAGCAAAAGGCGGTACTGGTGC
CAGGCAGAACCGAGTGGCGCCCAGCTCCGTTGCCCAAACCACGCGTTGACGGCCCGCACATGGCCACCGTCGTCGGCCCG
CCTGGCGAGGAAATCTACTGCGATGAATGGGGCCGGGTAAAAGTCAGTTTCCCCTGGGACCGCGAAAGCCAGAACAACGA
GTTCAGTTCGTGCTGGCTGCGGGTGTCTCAAGGCTGGGCGGGTGGCAGCTGGGGCTCGATGGCCATCCCCCGGATCGGCC
AGGACGTGATCATCCATTACGTCAACGGCGACCCCGATCAGCCGATGATCACCGGCCGTACTTACTGCGGCAACCAGCTG
CCACCGTACGACCTTCCCCGTCACAAGACCCGCATGACCATCAAGAGCCAGACCCACAAGGGCGATGGCTTCAACGAGCT
GCGCTTCGAGGACGAACTGGGCCAGGAGGAAGTGTTCATTCATGCCCAGAGGGACCAGAACAATGTGGTCAAGCATGATG
AGACTACGCAGGTCGGCAATGACCGCAAAGAGCAGGTCGATAACGACGAATCGATCCTGATCGGCCACGACCGTACGGAA
ACGGTTGGCAATGACGAGCGCATCACCATTGGGCAGGATGCCGCGCTCGACATCGGTAGAAACCACACGATCAGGATCGC
CAAGGACCGTGTCGAAAACATCGGCAATCACCGTCACGATCAGATCGCCGCCAATCACCACACCCGTATCGGTGGCAACT
TCGAGCAACGGGTCGAAGGGCATGCCGAGCTGGAAGCCCGTGGGGATATCCGCAGACGTACTCGCCGTTATGAACTTCGA
GCATCGGAAGCCGTGGTCATCCAAGGCCCTGGTGGCTCCATTCGAATCGACGAAACAGGCATCACACTGGACAGCCCGAC
GATTCGCATCACCGGCCAGATGCTCAAGTCGGCAAATGGAACGACAAGCCCCTTTTCCATCAGCAGTGCACCGAACACTG
GCAAGCCGCTGGAGCGCCTGTGTGGGCGCCGCCCTGATGGCTCCTGTCCCCTACCCGATTGCCGCTGTATCCGAGGGCAA
GTCCAATGA					