Detailed information of component protein


Summary

Component ID T6CP009510
Component protein type TssA
T6SS ID (Type) T6SS00396 experimental (Type i2)
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon chromosome
Sequence Protein sequence (533 a.a.); Nucleotide sequence (1599 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) ECL_RS08940
Coordinate (Strand) 1838342..1839940 (+)
NCBI ID WP_013096439.1
RefSeq NC_014121
Uniprot ID A0A0H3CI91_ENTCC
KEGG ID enc:ECL_01814
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 3.1e-70 231..488
ImpA_N PF06812.14 3.6e-22 33..138

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-532 MDILQNLVAACQADETLLRQQAQTRTERWLRWLAPVSVTCPTGEDPGYDDDFQRIREEVNKLSGIDTGLICTLAEKLMTTTAKDIRVATYYCWARLHQNGEAGFAEGLELVAGLLQRYGMQLHPRRDRSRKAALEWLGGTRVLDSLSLYPEVVREDAQRTAGTLLLIADSLETEPEALRAELNALYSALESRLMKGGGVDAVVPQNASNQTRSQQPSHTTEQDAPVLSRITSGQDLLAQARTLTGYLREQPGGWLAAHRLMKSLRHDTLSAIPAPDAEGKTRIEPPRADQRAMLKRLYLQQSWLEILEQADNTFSRGANHLWLDLQWYTHQALMKSGQDVLADIITADLKGLLRRLTGLETLAFNDGTPFADEVTLNWINQSVLDDMSGWRDEPVSAISTGDNDILALEPEALEKADSEGLDATLHWLQTRPGTDTKDRWLLRLLMARVAEQKGKNELALHLLGELDNAAQSITLAQWTPALLFEVKSRRFRLLCIKATRSEADKSRLQPEMDQLLTGLIALDPAGSAVLCG



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9278 - -



  Protein sequence: 533 a.a.    

>T6CP009510 NC_014121:1838342-1839940 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssA]
MDILQNLVAACQADETLLRQQAQTRTERWLRWLAPVSVTCPTGEDPGYDDDFQRIREEVNKLSGIDTGLICTLAEKLMTT
TAKDIRVATYYCWARLHQNGEAGFAEGLELVAGLLQRYGMQLHPRRDRSRKAALEWLGGTRVLDSLSLYPEVVREDAQRT
AGTLLLIADSLETEPEALRAELNALYSALESRLMKGGGVDAVVPQNASNQTRSQQPSHTTEQDAPVLSRITSGQDLLAQA
RTLTGYLREQPGGWLAAHRLMKSLRHDTLSAIPAPDAEGKTRIEPPRADQRAMLKRLYLQQSWLEILEQADNTFSRGANH
LWLDLQWYTHQALMKSGQDVLADIITADLKGLLRRLTGLETLAFNDGTPFADEVTLNWINQSVLDDMSGWRDEPVSAIST
GDNDILALEPEALEKADSEGLDATLHWLQTRPGTDTKDRWLLRLLMARVAEQKGKNELALHLLGELDNAAQSITLAQWTP
ALLFEVKSRRFRLLCIKATRSEADKSRLQPEMDQLLTGLIALDPAGSAVLCG*

  Nucleotide sequence: 1599 bp    

>T6CP009510 NC_014121:1838342-1839940 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssA]
ATGGATATTTTGCAGAATCTGGTGGCCGCCTGTCAGGCGGATGAAACCCTGTTGCGACAGCAGGCGCAGACTCGTACGGA
ACGCTGGCTGCGCTGGCTTGCTCCGGTCAGCGTGACCTGTCCGACGGGAGAAGACCCGGGATATGACGATGATTTCCAGC
GCATTCGCGAGGAGGTCAATAAGCTTTCCGGCATCGATACCGGGCTGATTTGCACGCTTGCCGAAAAACTGATGACCACC
ACGGCAAAAGATATCCGGGTTGCGACTTATTACTGCTGGGCGCGCCTGCATCAGAATGGCGAAGCTGGCTTCGCCGAAGG
GCTTGAACTGGTGGCAGGATTACTGCAGCGCTACGGCATGCAGCTTCACCCCCGGCGGGACCGCAGCCGCAAGGCCGCGC
TGGAATGGCTCGGCGGTACCCGTGTACTGGACAGTTTGTCACTGTACCCGGAGGTCGTACGCGAGGATGCACAGCGTACC
GCCGGGACGCTACTGCTCATTGCTGACAGCCTGGAGACAGAGCCTGAAGCGTTACGGGCGGAGCTGAATGCCCTGTACAG
CGCTCTTGAGTCCCGTCTGATGAAAGGCGGGGGCGTGGATGCCGTGGTACCGCAGAACGCAAGCAATCAGACACGTAGTC
AGCAGCCATCTCACACGACAGAACAGGACGCGCCAGTGCTGAGCCGCATCACCTCGGGTCAGGACTTACTGGCTCAGGCT
CGCACCCTGACGGGGTACCTGCGTGAGCAACCCGGTGGCTGGCTTGCGGCGCACCGGCTGATGAAAAGCCTGCGTCATGA
CACGCTGAGCGCTATTCCGGCACCGGATGCTGAAGGGAAAACGCGCATCGAGCCGCCGCGAGCTGACCAGCGGGCGATGC
TCAAGCGCCTCTATCTGCAACAGAGCTGGCTGGAAATACTGGAGCAGGCGGACAATACCTTCTCGCGCGGTGCCAATCAC
CTCTGGCTGGATTTGCAGTGGTATACCCATCAGGCCCTGATGAAGTCGGGGCAGGATGTGCTGGCCGACATCATCACTGC
CGACCTGAAAGGGCTGCTGCGCCGTCTTACCGGGCTTGAAACCCTGGCCTTTAACGACGGGACACCGTTTGCCGATGAGG
TCACGCTGAACTGGATAAACCAGAGCGTGCTGGACGATATGTCCGGCTGGCGGGATGAACCTGTCAGCGCTATCAGTACT
GGTGATAACGATATTCTGGCGCTTGAACCTGAAGCACTGGAGAAAGCAGACAGCGAAGGACTGGATGCCACACTCCACTG
GCTGCAGACGCGCCCGGGGACCGACACCAAAGACAGATGGTTGCTGCGTCTGCTGATGGCCCGTGTGGCAGAGCAGAAAG
GTAAAAATGAACTGGCCCTGCATTTACTGGGTGAGCTCGACAATGCCGCACAGTCCATCACCCTCGCACAGTGGACGCCG
GCGCTGTTGTTTGAAGTGAAATCACGCCGCTTCCGACTGCTGTGCATAAAAGCCACACGCAGTGAAGCCGATAAGTCACG
GCTTCAGCCGGAGATGGACCAGCTTCTGACTGGTCTGATTGCGCTTGACCCGGCAGGCAGTGCGGTGCTTTGCGGGTAA