SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009482
Component protein type	TssA
T6SS ID (Type)	T6SS00037 (Type i2)
Strain	Escherichia coli CFT073
Replicon	chromosome
Sequence	Protein sequence (537 a.a.); Nucleotide sequence (1611 bp)
Reference	[1] Lloyd AL, Henderson TA, Vigil PD, et al. Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 2009 Jun;191(11):3469-81. doi: 10.1128/JB.01717-08. Epub 2009 Mar 27. PMID: 19329634 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Leiman PG, Basler M, Ramagopal UA, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4154-9. doi: 10.1073/pnas.0813360106. Epub 2009 Feb 27. PMID: 19251641

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C_RS16130
Coordinate (Strand)	3242520..3244130 (+)
NCBI ID	WP_001443097.1
RefSeq	NC_004431
Uniprot ID	A0A210P5N0_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasJ	PF16989.7	1.9e-80	226..488
ImpA_N	PF06812.14	1.4e-18	34..138

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-536	MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGEDPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGERGLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANIVAALNLLTVSFAAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPVDAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKTRLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGVPWDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGKAAVPTDDATNDVLALEPEALAMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVAEQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRHADKAALARKMDALLGSLVAIDPARTAVLCDTQHKD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9408	-	-

Protein sequence: 537 a.a.

						>T6CP009482 NC_004431:3242520-3244130 [Escherichia coli CFT073] [TssA]
MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGEDPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLT
QAKDVRIATYYIWARLHRDGERGLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
VAALNLLTVSFAAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPVDAPQVQTITSGRDLLDQAKVLA
RYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKTRLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDI
QWYLHQALTKAGVPWDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGKAAVPTDDA
TNDVLALEPEALAMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVAEQYGKNEMALLLLEELDTAAQGITLTQWEP
ELLFEVKARQLKLLRLRAHRHADKAALARKMDALLGSLVAIDPARTAVLCDTQHKD*					

Nucleotide sequence: 1611 bp

						>T6CP009482 NC_004431:3242520-3244130 [Escherichia coli CFT073] [TssA]
ATGAGTGAACTGTTGCAAAAGCTGACCCGTAGCTGTTTTGCCGACCGGGATGCACTGGATGTGGCGCGAACACAGGCGGC
GCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAGATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTTCTGC
GTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACG
CAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCGGAAGG
ACTGGCGCTGCTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGCCCGGCCAGCCGGAAAATGGCGC
TGGAGTGGCTGGCGGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGCAAAAGAGGATTTTGCGAACATT
GTTGCCGCCCTGAATCTGCTGACGGTTTCCTTCGCCGCGTGGCCCGAAGACCAGCATTCCCCCTCCCTGATGCCGCTGAT
TAACGCGCTGGAATCGCGTCTGGCACAGTCGGGCGGAATGAACGCGGTGGTGCCGCAGAACAGCAGCGGCGTTCCGGCAC
CGTCATCCCCGGTGGATGCGCCGCAGGTACAGACCATCACCTCCGGACGTGACCTGCTCGACCAGGCGAAAGTGCTGGCC
CGTTATCTGAACGAACAGCCGCAGGGCTGGCTTTCGGCGCACCGGCTGATGAAAACCCTGCGCTGGGATACGGTGCATGA
ACTGCCGCCGGACGTGGACGGCAAAACCCGGCTGGCCCCGCCGCGCACCGAGTCACGCAACCAGCTTAAGCGGCTGTATG
CCCAGCAGAACTGGACGGAACTGCTGGAGCAGGCCGACCTGATGTTCAGCACCGGCGTCAGTCATTTCTGGCTGGACATT
CAGTGGTATCTGCATCAGGCGCTGACTAAGGCGGGCGTACCGTGGGACAGGTGGACAGCCGTTATCCGTCAGGACCTGGC
GTTACTGCTGGAACGCCTGCCAGGGCTGGAAAATCTGGCATGGAACGACGGCACACCGTTCGCCGACGAGGTGACGCGCA
ACTGGATAGCGCAGCAGGTCATGATGCGGGAAGACGGCGCATGGCTTGCGGGGAAAGCCGCTGTACCGACAGACGACGCC
ACAAATGACGTACTGGCGCTGGAGCCGGAAGCACTGGCGATGGCAGACAGTCAGGGCGTGGAAGCGGCGCTGGGCTGGAT
ACAGACCCGTCCGGGCATCACCACGGCACGACAGCGCCTGTTATTGCGGCTGCTGATGGCACGGGTGGCGGAACAGTACG
GTAAAAACGAGATGGCGCTCCTGCTGCTGGAAGAACTGGATACCGCCGCACAGGGAATAACGCTGACGCAGTGGGAGCCA
GAACTGCTGTTTGAAGTAAAAGCCCGGCAACTGAAACTGCTGCGCCTGCGGGCACATCGCCACGCGGACAAGGCGGCGCT
TGCCCGGAAAATGGACGCTCTGCTGGGTTCGCTGGTGGCAATCGATCCGGCGCGGACAGCGGTACTGTGTGATACGCAGC
ATAAGGACTGA					