SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009457
Component protein type	TssA
T6SS ID (Type)	T6SS00344 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (533 a.a.); Nucleotide sequence (1599 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS11300
Coordinate (Strand)	2321926..2323524 (+)
NCBI ID	WP_015874732.1
RefSeq	NC_012731
Uniprot ID	A0A1F2LXG3_9ENTR, A0A652Q190_9ENTR, A0A6I4LK35_9ENTR, A0A0J4XRE9_KLEPN, A0A3F3BQV4_KLEPN
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasJ	PF16989.7	5.1e-67	230..488
ImpA_N	PF06812.14	3.9e-23	33..137

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-532	MSILQNLVAASQLDESVLRQQARSRQAQWQSWLSPISDSQPTGSDPGYDDDFQRIREEVNKISGVDTVLICQLAGQLLATTKDIRIVTYYCWARLHQDGEAGLADGLELLAGLLQRYGMQLYPQRDRSRKAAIEWLAGTRILDSLSLFPEVVREDARRTVGALLLIDDCLTTEPETSRPDLQALWGALESRLIKGGGVDAVVPQNVGNRGLAQTASRMAEYEAPVLSNITSGQDLLAQARTLTAYLRVQPDGWLAAHRLMKSLRHDTLTIIPAPDAEGKTRIEPPRADQRAMLKRLYLQQSWSEILEQADSTFSRGANHLWLDLQWYIHQALVRSGQDVLADIIIADLKGLLRRLTGLETLAFNDGTPFADEVTLNWINQSVLDDMSVWQDEPVSAASGADNDILALEPEALEKADTDGLDAALHWIQTRPGTESTKEKWLLRLLMARVAEQKGKNDLALYLLRELDDAAGSITLTQWIPALLFEIKSRRLRLLRMKATRSESEKLRLQPEMDQLLAGLIALDPASSAVLCG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9766	-	-

Protein sequence: 533 a.a.

						>T6CP009457 NC_012731:2321926-2323524 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssA]
MSILQNLVAASQLDESVLRQQARSRQAQWQSWLSPISDSQPTGSDPGYDDDFQRIREEVNKISGVDTVLICQLAGQLLAT
TKDIRIVTYYCWARLHQDGEAGLADGLELLAGLLQRYGMQLYPQRDRSRKAAIEWLAGTRILDSLSLFPEVVREDARRTV
GALLLIDDCLTTEPETSRPDLQALWGALESRLIKGGGVDAVVPQNVGNRGLAQTASRMAEYEAPVLSNITSGQDLLAQAR
TLTAYLRVQPDGWLAAHRLMKSLRHDTLTIIPAPDAEGKTRIEPPRADQRAMLKRLYLQQSWSEILEQADSTFSRGANHL
WLDLQWYIHQALVRSGQDVLADIIIADLKGLLRRLTGLETLAFNDGTPFADEVTLNWINQSVLDDMSVWQDEPVSAASGA
DNDILALEPEALEKADTDGLDAALHWIQTRPGTESTKEKWLLRLLMARVAEQKGKNDLALYLLRELDDAAGSITLTQWIP
ALLFEIKSRRLRLLRMKATRSESEKLRLQPEMDQLLAGLIALDPASSAVLCG*					

Nucleotide sequence: 1599 bp

						>T6CP009457 NC_012731:2321926-2323524 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssA]
ATGAGCATTTTACAAAATTTGGTTGCCGCCAGTCAGTTGGATGAAAGCGTACTGCGCCAGCAGGCCCGGTCACGTCAGGC
GCAGTGGCAGTCCTGGCTTTCGCCAATCAGTGATAGCCAGCCCACCGGGAGCGATCCGGGCTATGACGATGATTTTCAGC
GTATCCGCGAGGAGGTCAATAAGATCTCCGGTGTCGATACCGTACTGATTTGCCAGTTGGCTGGACAGTTACTGGCAACA
ACAAAAGATATCCGGATTGTTACCTATTACTGCTGGGCCCGTTTGCACCAGGACGGGGAAGCTGGCTTGGCCGATGGGCT
GGAGCTGCTGGCAGGCTTGCTGCAACGATATGGTATGCAGCTTTATCCGCAGCGCGACCGTAGCCGCAAAGCAGCCATCG
AGTGGCTGGCGGGTACTCGTATCTTAGACAGTCTTTCACTTTTTCCAGAAGTTGTGCGGGAGGACGCAAGGCGTACCGTC
GGCGCGCTGCTTCTCATTGATGATTGTCTGACGACAGAACCAGAAACATCACGGCCAGATCTGCAAGCACTCTGGGGTGC
ACTGGAGTCTCGTTTGATAAAGGGCGGGGGAGTCGATGCGGTGGTGCCGCAGAACGTCGGAAACCGGGGGCTGGCTCAAA
CCGCTTCACGCATGGCTGAATACGAAGCGCCGGTTCTCAGTAACATCACCTCCGGTCAGGATCTGCTGGCCCAGGCGCGA
ACCCTGACAGCATATCTGCGTGTTCAACCCGATGGCTGGCTTGCCGCACATCGGCTAATGAAAAGCCTTCGTCATGACAC
CTTGACAATCATCCCTGCGCCTGATGCTGAAGGAAAAACACGTATCGAGCCTCCCAGAGCAGATCAGCGTGCCATGCTCA
AGCGGCTGTACCTGCAACAGAGCTGGTCGGAGATACTGGAACAGGCGGACAGTACCTTCTCACGTGGCGCTAACCACCTC
TGGCTTGACCTGCAATGGTATATCCATCAGGCGCTGGTGAGGTCGGGTCAGGATGTGCTGGCCGATATTATAATTGCCGA
TCTGAAAGGATTGCTGCGCCGTCTCACCGGGCTTGAAACATTAGCTTTTAACGACGGCACACCCTTTGCCGATGAGGTCA
CGCTGAACTGGATAAATCAGAGTGTCCTCGACGATATGTCCGTCTGGCAGGATGAGCCAGTATCGGCTGCCAGCGGCGCT
GATAACGACATTCTGGCCCTTGAACCTGAAGCGCTCGAGAAGGCTGACACTGACGGTCTCGATGCTGCACTCCACTGGAT
TCAGACGCGCCCGGGCACTGAATCCACTAAAGAAAAATGGCTGTTGCGCCTGCTTATGGCCCGCGTGGCCGAACAAAAGG
GGAAGAATGACCTCGCGCTCTATCTTCTGCGAGAACTCGACGATGCGGCGGGATCAATCACCCTCACGCAGTGGATCCCA
GCCCTGTTGTTCGAGATTAAATCGCGACGGCTCAGGCTACTGCGCATGAAAGCCACGCGCAGCGAGAGTGAAAAGTTACG
ATTGCAACCGGAGATGGATCAATTGCTGGCAGGTCTGATTGCGCTCGATCCAGCCAGTAGCGCGGTGCTTTGCGGCTAA					