SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009456
Component protein type	TssD
T6SS ID (Type)	T6SS00344 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS11245
Coordinate (Strand)	2304685..2305176 (+)
NCBI ID	WP_002902160.1
RefSeq	NC_012731
Uniprot ID	A0A0H3GMC3_KLEPH, A0A7U7IJ29_KLEPN, W1DW38_KLEPN, W1ERT8_KLEPN, W1HVR0_KLEPN, A0A1F2LWX0_9ENTR, A0A223UC57_9ENTR, A0A264BVD1_KLEVA, A0A7U2ZMJ7_9ENTR, A0A652Q1A1_9ENTR, A0A6I4LNL9_9ENTR, A6T838_KLEP7, A0A3S4LPK0_KLEAE, A0A0J2G7E8_KLEPN, A0A1Y0Q1G9_KLEPN
KEGG ID	kpg:KPNIH32_11835, kpm:KPHS_23020, kpx:PMK1_03725, kpnu:LI86_14600, kvq:SP68_00350, kpw:KPNIH30_11995, kpn:KPN_01327, kpy:KPNIH31_11050, kpb:FH42_02920, kpt:VK055_1101, kpne:KU54_014665
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	1.5e-37	6..146

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-163	MAIPVYLWLKDDGGADIKGSVDVQDREGSIEVVAQEHNLYIPTDNNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTKLENVKVVKVNPLMHDIKNPAYEKHNHLEQIELRYEKITWTYKDGNIIHSDSWNERATA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9879	-	-

Protein sequence: 164 a.a.

						>T6CP009456 NC_012731:2304685-2305176 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssD]
MAIPVYLWLKDDGGADIKGSVDVQDREGSIEVVAQEHNLYIPTDNNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQ
TLKSAEFKWYRINDAGQEVEYFNTKLENVKVVKVNPLMHDIKNPAYEKHNHLEQIELRYEKITWTYKDGNIIHSDSWNER
ATA*					

Nucleotide sequence: 492 bp

						>T6CP009456 NC_012731:2304685-2305176 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssD]
ATGGCAATTCCTGTTTATCTTTGGCTGAAAGACGACGGCGGCGCGGACATTAAAGGGTCTGTCGACGTTCAGGATCGTGA
AGGCAGTATCGAAGTGGTGGCCCAGGAGCATAACCTGTACATCCCGACCGATAACAACACCGGCAAACTGACCGGCACGC
GTATTCATACGCCGTTCCTGTTCACCAAGGAGATCGACTCCTCCAGCCCCTACCTGTACAAGGCGGTGACCACCGGCCAG
ACCCTGAAATCCGCCGAGTTCAAGTGGTACCGAATCAACGATGCCGGCCAGGAAGTGGAGTATTTCAATACCAAACTTGA
GAACGTCAAGGTGGTGAAAGTGAATCCCCTGATGCACGACATCAAAAACCCCGCCTACGAGAAGCACAACCATCTGGAAC
AGATTGAGCTGCGCTACGAGAAGATCACCTGGACCTATAAAGACGGCAACATCATTCACTCCGATAGCTGGAACGAACGC
GCCACCGCGTAA					