Detailed information of component protein
Summary
| Component ID | T6CP009344 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00346  (Type i4b) |
| Strain | Edwardsiella ictaluri 93-146 |
| Replicon | chromosome |
| Sequence | Protein sequence (217 a.a.); Nucleotide sequence (651 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | NT01EI_RS11955 |
| Coordinate (Strand) | 2647288..2647938 (+) |
| NCBI ID | WP_015872019.1 |
| RefSeq | NC_012779 |
| Uniprot ID | C5BAE8_EDWI9 |
| KEGG ID | eic:NT01EI_2750 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 5.2e-39 | 3..202 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-182 | VSLSEAFIQPMLYVRQFLQAPEGDASLFRERLRSYLHLSRSRAQEGGETPATIDSAMYAVVAWIDETIMCSDWDGVATWRREPLQASYFNTVCAGVDFFDRLSALSPEADDVREVYLLCLALGFEGRHTGSMGQATLEQIRMHQLKTLHGEIWEESQRLFPEAYPIPSVKPPQQKRLLTNSR |
| Transmembrane helix | 183-205 | FTTIVVPLVILVALYGILNWVLY |
| Outside | 206-216 | EYADVALELLP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9925 | - | - |
Protein sequence: 217 a.a.
>T6CP009344 NC_012779:2647288-2647938 [Edwardsiella ictaluri 93-146] [TssL]
VSLSEAFIQPMLYVRQFLQAPEGDASLFRERLRSYLHLSRSRAQEGGETPATIDSAMYAVVAWIDETIMCSDWDGVATWR
REPLQASYFNTVCAGVDFFDRLSALSPEADDVREVYLLCLALGFEGRHTGSMGQATLEQIRMHQLKTLHGEIWEESQRLF
PEAYPIPSVKPPQQKRLLTNSRFTTIVVPLVILVALYGILNWVLYEYADVALELLP*
VSLSEAFIQPMLYVRQFLQAPEGDASLFRERLRSYLHLSRSRAQEGGETPATIDSAMYAVVAWIDETIMCSDWDGVATWR
REPLQASYFNTVCAGVDFFDRLSALSPEADDVREVYLLCLALGFEGRHTGSMGQATLEQIRMHQLKTLHGEIWEESQRLF
PEAYPIPSVKPPQQKRLLTNSRFTTIVVPLVILVALYGILNWVLYEYADVALELLP*
Nucleotide sequence: 651 bp
>T6CP009344 NC_012779:2647288-2647938 [Edwardsiella ictaluri 93-146] [TssL]
GTGAGTTTATCGGAAGCATTTATTCAGCCCATGCTCTATGTGCGGCAATTTCTACAGGCGCCCGAAGGGGATGCATCATT
ATTTCGTGAACGGCTGCGATCATACCTGCACCTCAGCCGGAGCCGGGCCCAGGAGGGAGGGGAAACCCCGGCCACGATCG
ATTCCGCTATGTATGCCGTGGTGGCCTGGATAGATGAGACGATCATGTGTTCGGACTGGGACGGCGTTGCAACATGGCGC
CGTGAGCCACTGCAGGCCAGTTACTTCAACACCGTCTGCGCCGGAGTTGACTTTTTCGACAGGCTGTCCGCACTGAGCCC
CGAAGCCGACGACGTCAGAGAAGTCTATCTGCTGTGTCTGGCTCTGGGATTTGAGGGGCGCCATACCGGCAGCATGGGTC
AGGCCACGCTGGAACAGATCAGGATGCACCAACTGAAGACCCTGCACGGCGAGATATGGGAGGAGTCACAGCGTCTATTT
CCGGAGGCCTATCCGATACCCTCAGTCAAACCGCCGCAGCAAAAGCGATTGCTGACCAACAGCCGTTTTACCACCATCGT
GGTGCCGCTGGTCATATTGGTCGCACTGTATGGAATTCTGAACTGGGTATTATATGAGTATGCGGATGTCGCGCTGGAGT
TGCTGCCATGA
GTGAGTTTATCGGAAGCATTTATTCAGCCCATGCTCTATGTGCGGCAATTTCTACAGGCGCCCGAAGGGGATGCATCATT
ATTTCGTGAACGGCTGCGATCATACCTGCACCTCAGCCGGAGCCGGGCCCAGGAGGGAGGGGAAACCCCGGCCACGATCG
ATTCCGCTATGTATGCCGTGGTGGCCTGGATAGATGAGACGATCATGTGTTCGGACTGGGACGGCGTTGCAACATGGCGC
CGTGAGCCACTGCAGGCCAGTTACTTCAACACCGTCTGCGCCGGAGTTGACTTTTTCGACAGGCTGTCCGCACTGAGCCC
CGAAGCCGACGACGTCAGAGAAGTCTATCTGCTGTGTCTGGCTCTGGGATTTGAGGGGCGCCATACCGGCAGCATGGGTC
AGGCCACGCTGGAACAGATCAGGATGCACCAACTGAAGACCCTGCACGGCGAGATATGGGAGGAGTCACAGCGTCTATTT
CCGGAGGCCTATCCGATACCCTCAGTCAAACCGCCGCAGCAAAAGCGATTGCTGACCAACAGCCGTTTTACCACCATCGT
GGTGCCGCTGGTCATATTGGTCGCACTGTATGGAATTCTGAACTGGGTATTATATGAGTATGCGGATGTCGCGCTGGAGT
TGCTGCCATGA