SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009341
Component protein type	TssJ
T6SS ID (Type)	T6SS00464 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (184 a.a.); Nucleotide sequence (552 bp)
Reference	[1] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558 [2] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS24410
Coordinate (Strand)	4902569..4903120 (+)
NCBI ID	WP_000152745.1
RefSeq	NC_017626
Uniprot ID	D3GUZ9_ECO44, A0A447X889_ECOLX
KEGG ID	elo:EC042_4573
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	2.5e-07	100..149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-183	MTIKCLLLAVMVCPFFITACSDKYSEQDRVQAINNADAPFGAGLLTFQISSDTQLNSLNEISNSCALLFLQASDKNTLQDIMNNPVLIKQFFIGTGKVDGILKVDKYVAMPGQEITLHIDRNEGTKYVGVVAGYYPFPGKQHMLLLDIPVDVVEEGWWNKSLHASLLPLSKKISMGKESISMK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9937	pos: 19-20. ITA-CS.	MTIKCLLLAVMVCPFFITA

Protein sequence: 184 a.a.

						>T6CP009341 NC_017626:4902569-4903120 [Escherichia coli 042] [TssJ]
MTIKCLLLAVMVCPFFITACSDKYSEQDRVQAINNADAPFGAGLLTFQISSDTQLNSLNEISNSCALLFLQASDKNTLQD
IMNNPVLIKQFFIGTGKVDGILKVDKYVAMPGQEITLHIDRNEGTKYVGVVAGYYPFPGKQHMLLLDIPVDVVEEGWWNK
SLHASLLPLSKKISMGKESISMK*					

Nucleotide sequence: 552 bp

						>T6CP009341 NC_017626:4902569-4903120 [Escherichia coli 042] [TssJ]
ATGACTATTAAGTGCCTGCTACTAGCGGTTATGGTATGCCCCTTTTTCATCACTGCCTGTTCTGATAAGTATTCTGAACA
GGATAGAGTGCAGGCTATCAACAATGCCGATGCTCCATTTGGCGCCGGCTTGCTTACTTTTCAGATATCATCTGATACGC
AATTAAACTCACTTAACGAGATATCCAACAGCTGTGCATTATTATTCTTACAGGCTTCAGATAAAAATACATTACAGGAC
ATAATGAATAATCCTGTGTTAATCAAACAGTTTTTTATCGGGACAGGTAAGGTTGATGGCATTCTTAAAGTTGATAAATA
CGTGGCTATGCCGGGGCAGGAGATAACGCTACATATAGACAGGAATGAAGGGACTAAATATGTAGGTGTGGTTGCTGGAT
ACTATCCATTTCCTGGAAAACAGCATATGTTACTCTTAGATATTCCGGTTGATGTTGTTGAGGAAGGTTGGTGGAATAAA
TCATTACATGCATCACTCTTACCTCTTTCCAAAAAGATATCGATGGGTAAAGAAAGTATCAGTATGAAATAA					