Detailed information of component protein
Summary
| Component ID | T6CP009321 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00396  (Type i2) |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon | chromosome |
| Sequence | Protein sequence (231 a.a.); Nucleotide sequence (693 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | ECL_RS08890 |
| Coordinate (Strand) | 1822895..1823587 (+) |
| NCBI ID | WP_013096431.1 |
| RefSeq | NC_014121 |
| Uniprot ID | A0A0M7BW79_ENTCL, A0A0H3CJM2_ENTCC |
| KEGG ID | enc:ECL_01804 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 6.7e-31 | 14..215 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-190 | MSERKRGTAAAIDIDALLQDTWLQVISLRHGPTFQEGEGRTLWERCIADVERVQRELKASELDEASCQHILTAQCALLDEAVKGRGVEDDACVQWYDIPLQGHFLGTMDAGDTLCDRMRDVLREPAPDHAVVTCFQRVMMLGFLGSYRSLNDPERQKLVNALSEYVTPFSYPQSYPVLAESHTGRGIMGG |
| Transmembrane helix | 191-213 | WLASWPVRIGLSVIVVAALWWGL |
| Outside | 214-230 | DRWLDQLLLTLLPGAVK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9951 | - | - |
Protein sequence: 231 a.a.
>T6CP009321 NC_014121:1822895-1823587 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssL]
MSERKRGTAAAIDIDALLQDTWLQVISLRHGPTFQEGEGRTLWERCIADVERVQRELKASELDEASCQHILTAQCALLDE
AVKGRGVEDDACVQWYDIPLQGHFLGTMDAGDTLCDRMRDVLREPAPDHAVVTCFQRVMMLGFLGSYRSLNDPERQKLVN
ALSEYVTPFSYPQSYPVLAESHTGRGIMGGWLASWPVRIGLSVIVVAALWWGLDRWLDQLLLTLLPGAVK*
MSERKRGTAAAIDIDALLQDTWLQVISLRHGPTFQEGEGRTLWERCIADVERVQRELKASELDEASCQHILTAQCALLDE
AVKGRGVEDDACVQWYDIPLQGHFLGTMDAGDTLCDRMRDVLREPAPDHAVVTCFQRVMMLGFLGSYRSLNDPERQKLVN
ALSEYVTPFSYPQSYPVLAESHTGRGIMGGWLASWPVRIGLSVIVVAALWWGLDRWLDQLLLTLLPGAVK*
Nucleotide sequence: 693 bp
>T6CP009321 NC_014121:1822895-1823587 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssL]
ATGAGTGAGCGTAAACGCGGCACTGCCGCGGCCATTGATATTGATGCCCTGCTGCAGGACACCTGGCTTCAGGTGATCAG
CCTGCGTCACGGTCCGACGTTTCAGGAGGGCGAAGGGCGCACGCTGTGGGAGCGCTGTATTGCTGATGTCGAACGTGTGC
AGCGTGAGCTGAAAGCGAGCGAACTCGATGAAGCCAGCTGTCAGCATATTCTTACCGCACAGTGCGCCCTGCTCGATGAG
GCGGTGAAAGGTCGTGGTGTGGAAGATGACGCCTGTGTGCAGTGGTATGACATTCCCCTGCAGGGGCACTTCCTCGGCAC
TATGGACGCCGGCGACACGCTGTGCGATCGGATGCGCGATGTGCTGCGTGAACCGGCGCCTGACCACGCTGTCGTGACCT
GCTTCCAGCGGGTCATGATGCTGGGTTTTCTCGGCAGTTACCGATCATTGAATGACCCGGAGCGCCAGAAACTTGTCAAC
GCACTCAGTGAATATGTCACACCGTTCAGCTATCCACAGTCCTACCCCGTACTGGCGGAAAGTCATACCGGGCGGGGAAT
CATGGGCGGCTGGCTGGCGTCATGGCCCGTACGTATTGGTCTGAGCGTGATAGTGGTCGCTGCACTCTGGTGGGGACTGG
ATCGCTGGCTGGATCAGTTGCTGCTAACCCTGTTGCCGGGGGCCGTGAAATGA
ATGAGTGAGCGTAAACGCGGCACTGCCGCGGCCATTGATATTGATGCCCTGCTGCAGGACACCTGGCTTCAGGTGATCAG
CCTGCGTCACGGTCCGACGTTTCAGGAGGGCGAAGGGCGCACGCTGTGGGAGCGCTGTATTGCTGATGTCGAACGTGTGC
AGCGTGAGCTGAAAGCGAGCGAACTCGATGAAGCCAGCTGTCAGCATATTCTTACCGCACAGTGCGCCCTGCTCGATGAG
GCGGTGAAAGGTCGTGGTGTGGAAGATGACGCCTGTGTGCAGTGGTATGACATTCCCCTGCAGGGGCACTTCCTCGGCAC
TATGGACGCCGGCGACACGCTGTGCGATCGGATGCGCGATGTGCTGCGTGAACCGGCGCCTGACCACGCTGTCGTGACCT
GCTTCCAGCGGGTCATGATGCTGGGTTTTCTCGGCAGTTACCGATCATTGAATGACCCGGAGCGCCAGAAACTTGTCAAC
GCACTCAGTGAATATGTCACACCGTTCAGCTATCCACAGTCCTACCCCGTACTGGCGGAAAGTCATACCGGGCGGGGAAT
CATGGGCGGCTGGCTGGCGTCATGGCCCGTACGTATTGGTCTGAGCGTGATAGTGGTCGCTGCACTCTGGTGGGGACTGG
ATCGCTGGCTGGATCAGTTGCTGCTAACCCTGTTGCCGGGGGCCGTGAAATGA