SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009313
Component protein type	TssL
T6SS ID (Type)	T6SS00345 (Type Unknown)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (218 a.a.); Nucleotide sequence (654 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS15785
Coordinate (Strand)	3212882..3213535 (-)
NCBI ID	WP_004891113.1
RefSeq	NC_012731
Uniprot ID	A0A652PZ08_9ENTR, W9BPL8_KLEPN, A0A3F3B3W0_KLEPN
KEGG ID	kpnk:BN49_3375
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.9e-36	12..202

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-184	MNTSESDLINKTFYPGWLMVSQLRCGQPVTDGEALYRQACRWVTEAREALTAGGVSEASAEQMLYAYCALLDESVLNRASQDDGYRRWRKDPLQARFFSTLNAGEELWERIRQLLREPTADAAVLTCFFRTLQLGFVGQYRAEDDERREDVAQALGARVPPFSLTQEAPVVVRASRLRSGRRMY
Transmembrane helix	185-207	WCGWAAGIVALAALWLTCSAMLS
Outside	208-217	QMVAQIAGQG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9588	-	-

Protein sequence: 218 a.a.

						>T6CP009313 NC_012731:c3213535-3212882 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssL]
MNTSESDLINKTFYPGWLMVSQLRCGQPVTDGEALYRQACRWVTEAREALTAGGVSEASAEQMLYAYCALLDESVLNRAS
QDDGYRRWRKDPLQARFFSTLNAGEELWERIRQLLREPTADAAVLTCFFRTLQLGFVGQYRAEDDERREDVAQALGARVP
PFSLTQEAPVVVRASRLRSGRRMYWCGWAAGIVALAALWLTCSAMLSQMVAQIAGQG*					

Nucleotide sequence: 654 bp

						>T6CP009313 NC_012731:c3213535-3212882 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [TssL]
ATGAATACCTCTGAAAGCGATCTGATCAACAAAACTTTTTACCCGGGCTGGCTGATGGTCAGCCAGTTACGCTGCGGCCA
GCCGGTGACGGATGGCGAGGCGCTCTATCGCCAGGCCTGTCGATGGGTGACCGAGGCGCGCGAGGCGCTGACGGCGGGGG
GAGTCAGTGAAGCCAGCGCTGAGCAGATGCTCTATGCCTACTGCGCGCTGCTGGATGAGAGCGTCCTCAACCGCGCCAGT
CAGGATGATGGCTACCGCAGGTGGCGCAAGGATCCGCTGCAGGCGCGCTTTTTCAGCACTCTCAACGCCGGGGAAGAGCT
CTGGGAACGGATCCGCCAGCTGCTGCGAGAGCCCACGGCGGATGCGGCAGTGCTGACCTGCTTCTTTCGTACTTTACAGC
TGGGATTTGTCGGACAGTACCGCGCCGAGGATGACGAACGGCGTGAGGACGTGGCGCAAGCGCTTGGCGCGCGGGTCCCG
CCGTTCAGCCTGACCCAGGAGGCGCCGGTGGTGGTTCGTGCCTCCCGGCTGCGCAGCGGACGGCGGATGTACTGGTGCGG
CTGGGCGGCTGGCATTGTGGCGCTGGCGGCGCTGTGGTTGACCTGCTCCGCCATGCTGTCACAGATGGTGGCGCAGATAG
CAGGGCAGGGATAA					