SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009289
Component protein type	TssL
T6SS ID (Type)	T6SS00019 (Type i2)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (229 a.a.); Nucleotide sequence (687 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS08325
Coordinate (Strand)	1659848..1660534 (+)
NCBI ID	WP_002210015.1
RefSeq	NC_003143
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	3.6e-43	14..215

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-192	MNPHLTEHPPVGDIDALLQDTWLQVISLRQGVTCAEGEGQAFWQRCVADIERVHQALKDAGHSEQSCQHIRYAQCALLDETVKGRGVQDDAYFVWCHSPLQAHFFNTLDAGSQLYERMRAVLREPAPDRAVLTCFHRVLMLGFLGGYASPAASEREQLIDQLSVQVPAFSVAPSRGILASAASRNRLGIWLR
Transmembrane helix	193-212	YWPVRLGLAALMVALLWWGL
Outside	213-228	DHWLSGLLATLLPEPV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9900	-	-

Protein sequence: 229 a.a.

						>T6CP009289 NC_003143:1659848-1660534 [Yersinia pestis CO92] [TssL]
MNPHLTEHPPVGDIDALLQDTWLQVISLRQGVTCAEGEGQAFWQRCVADIERVHQALKDAGHSEQSCQHIRYAQCALLDE
TVKGRGVQDDAYFVWCHSPLQAHFFNTLDAGSQLYERMRAVLREPAPDRAVLTCFHRVLMLGFLGGYASPAASEREQLID
QLSVQVPAFSVAPSRGILASAASRNRLGIWLRYWPVRLGLAALMVALLWWGLDHWLSGLLATLLPEPV*					

Nucleotide sequence: 687 bp

						>T6CP009289 NC_003143:1659848-1660534 [Yersinia pestis CO92] [TssL]
ATGAATCCTCACCTAACCGAACACCCCCCAGTCGGGGATATTGACGCCCTGTTGCAGGACACCTGGCTACAGGTGATCAG
CCTGCGTCAAGGGGTAACCTGTGCCGAGGGCGAAGGGCAGGCATTCTGGCAGCGCTGTGTGGCGGACATTGAACGTGTCC
ATCAGGCGCTGAAAGACGCCGGTCACAGCGAGCAGAGTTGCCAGCACATCCGATACGCCCAATGTGCACTGCTGGATGAG
ACCGTGAAAGGGCGCGGTGTACAGGATGATGCCTACTTCGTGTGGTGCCATTCGCCCTTACAGGCGCATTTTTTCAATAC
CCTGGATGCCGGTAGCCAGCTTTACGAGCGGATGCGCGCCGTGTTGCGTGAGCCTGCGCCGGATCGCGCGGTACTGACCT
GTTTTCACCGGGTATTGATGTTGGGCTTCCTCGGCGGGTACGCCTCCCCGGCGGCTTCTGAACGGGAGCAACTGATCGAT
CAACTGAGCGTGCAGGTACCGGCGTTCAGTGTCGCGCCATCACGTGGGATCCTGGCAAGCGCCGCCTCCCGCAACCGGTT
GGGGATCTGGTTGCGTTACTGGCCGGTACGGCTCGGGCTGGCTGCGCTGATGGTTGCGCTGTTGTGGTGGGGCCTTGATC
ACTGGCTGTCCGGTCTGTTAGCCACCTTACTGCCGGAGCCGGTGTAA					