SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009063
Component protein type	TssM
T6SS ID (Type)	T6SS00464 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (1135 a.a.); Nucleotide sequence (3405 bp)
Reference	[1] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558 [2] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS24430
Coordinate (Strand)	4905486..4908890 (+)
NCBI ID	WP_001403210.1
RefSeq	NC_017626
Uniprot ID	UPI0002A3B19C
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	1.1e-16	174..341

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-4	MLKK
Transmembrane helix	5-27	TFSLFLWFFVSVFVLSVCYVIGV
Inside	28-38	FRNWTDTTTAL
Transmembrane helix	39-61	YWISVILVTLSFKFIMDCIHNYF
Outside	62-1134	SAGKYRNFLKSFSRKKRALTLDSYFKKGLNIISKRGRGEVPWFLLTGDSEKNNSLLEDINLPVFHRNCVNSTPQQFRTIRWWFFRDLSVLELSGKIYDNPGVINSVISSLSARKCKKQPPRGVVLVISADKLINHDYTYIRTLSQKTRSFVEQLSLHLHHNIPVFIVISGCEHISGYSVLAQKIYQKHEKWHPVFWATNKSGTNASEYDSSYILSSLRSNIMASICHALDDSLSDKEKNDILQFPDRLKGLKYSLDIFISTFCIENVFFSQTEIAGICLSGELMGEKDKNCIFADILISEILPQIHDSDVIHVNKQRVNVSFKSVIAMTALCFVGYSGYCSYNVYNIRHGSVDTSHAFLTEQISKYEDKVRSNMRYFPFKPALDDKYLFFRESLHKTTRFDISPVSWRVTEYKKNFMQASPSGKRELILSLSSSLISWDKMMKDESLSDLAKSPGIHELLKITRPHDKISSIASLAVERDEIQKNNGIENIYVFRNLLTELVQSDPSYSWFVSEDVNIPAVRITDFWEDENSSVYLSGIWTQPGQNKLHQWYETIKEAYGRDTVPEAFSSFVLYLDESRQEHFRQFIMSVARARKDSHSGLMNPLQLTNIIHNRSSEHRFFQFVDDELHNIPTSSAQDWLSEFRLLNHLFSLKVDNGMKRQIEQFDLMLRIYLISVLNNSQMNRTLTHVTTWRSWQNALRNAVNSVLHTASSVELIRNAMRSDPENKLVILFDEFEKVRSVINSNNREPVIDSVWDIYERQIYQLLDHAVTYTGCWVGEQWRNSVLGRFNSGKHNLSYSEMQGKVYKDIIGFLKGPSNGVLALDPDGVRLLSFRERSIPFSPSFITFINDIVSPDDLLDVWLRERTQNKDELINVQGQLDLLNQTLQNAESQPYRVTIDSAPATIPDNPRVKPTGTTLTLECKTGNSSIRSMNFADSGIFTWYPGSCHSVRIDILFPNFSATYKFTGETAWIDFINKFSDGESELMTKDFSPESRNFLESMGIKGILVRYKLSDTGNLSQAYIEWEQLKQEKDKLKDLQVNLSNKLLTTHSWEKSAWISRLPGNITICPVVQE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8234	-	-

Protein sequence: 1135 a.a.

						>T6CP009063 NC_017626:4905486-4908890 [Escherichia coli 042] [TssM]
MLKKTFSLFLWFFVSVFVLSVCYVIGVFRNWTDTTTALYWISVILVTLSFKFIMDCIHNYFSAGKYRNFLKSFSRKKRAL
TLDSYFKKGLNIISKRGRGEVPWFLLTGDSEKNNSLLEDINLPVFHRNCVNSTPQQFRTIRWWFFRDLSVLELSGKIYDN
PGVINSVISSLSARKCKKQPPRGVVLVISADKLINHDYTYIRTLSQKTRSFVEQLSLHLHHNIPVFIVISGCEHISGYSV
LAQKIYQKHEKWHPVFWATNKSGTNASEYDSSYILSSLRSNIMASICHALDDSLSDKEKNDILQFPDRLKGLKYSLDIFI
STFCIENVFFSQTEIAGICLSGELMGEKDKNCIFADILISEILPQIHDSDVIHVNKQRVNVSFKSVIAMTALCFVGYSGY
CSYNVYNIRHGSVDTSHAFLTEQISKYEDKVRSNMRYFPFKPALDDKYLFFRESLHKTTRFDISPVSWRVTEYKKNFMQA
SPSGKRELILSLSSSLISWDKMMKDESLSDLAKSPGIHELLKITRPHDKISSIASLAVERDEIQKNNGIENIYVFRNLLT
ELVQSDPSYSWFVSEDVNIPAVRITDFWEDENSSVYLSGIWTQPGQNKLHQWYETIKEAYGRDTVPEAFSSFVLYLDESR
QEHFRQFIMSVARARKDSHSGLMNPLQLTNIIHNRSSEHRFFQFVDDELHNIPTSSAQDWLSEFRLLNHLFSLKVDNGMK
RQIEQFDLMLRIYLISVLNNSQMNRTLTHVTTWRSWQNALRNAVNSVLHTASSVELIRNAMRSDPENKLVILFDEFEKVR
SVINSNNREPVIDSVWDIYERQIYQLLDHAVTYTGCWVGEQWRNSVLGRFNSGKHNLSYSEMQGKVYKDIIGFLKGPSNG
VLALDPDGVRLLSFRERSIPFSPSFITFINDIVSPDDLLDVWLRERTQNKDELINVQGQLDLLNQTLQNAESQPYRVTID
SAPATIPDNPRVKPTGTTLTLECKTGNSSIRSMNFADSGIFTWYPGSCHSVRIDILFPNFSATYKFTGETAWIDFINKFS
DGESELMTKDFSPESRNFLESMGIKGILVRYKLSDTGNLSQAYIEWEQLKQEKDKLKDLQVNLSNKLLTTHSWEKSAWIS
RLPGNITICPVVQE*					

Nucleotide sequence: 3405 bp

						>T6CP009063 NC_017626:4905486-4908890 [Escherichia coli 042] [TssM]
ATGCTTAAGAAGACATTTTCATTATTTCTCTGGTTTTTTGTTTCTGTCTTCGTTCTGTCTGTTTGCTATGTTATCGGCGT
GTTCAGAAACTGGACAGATACTACAACTGCACTTTACTGGATTTCTGTGATTCTTGTTACTCTTTCCTTTAAATTTATTA
TGGATTGTATCCATAATTACTTTTCTGCAGGAAAATACAGAAATTTTCTAAAGTCTTTCTCTCGTAAAAAGAGAGCATTG
ACGCTTGATTCGTATTTCAAAAAAGGTCTGAATATCATAAGTAAAAGGGGAAGAGGAGAGGTTCCTTGGTTCTTACTTAC
GGGAGATTCAGAAAAAAACAACTCTTTGCTTGAAGATATCAATCTTCCTGTCTTTCACCGTAATTGTGTAAATAGCACTC
CCCAACAATTCCGGACAATTCGTTGGTGGTTTTTCAGAGATTTAAGCGTGCTGGAGTTGTCAGGAAAGATATATGATAAC
CCGGGAGTAATAAACTCTGTGATAAGTTCTTTATCAGCACGTAAATGTAAAAAACAGCCACCTCGTGGCGTTGTTCTCGT
CATTTCTGCTGATAAGCTAATTAATCATGATTACACCTATATTCGGACATTGTCTCAAAAAACAAGGAGTTTTGTAGAAC
AACTGTCGTTACACTTACATCACAACATTCCGGTCTTTATAGTGATTAGTGGATGTGAGCACATCAGTGGATATTCAGTG
CTGGCACAAAAAATCTATCAAAAACATGAAAAATGGCATCCAGTGTTTTGGGCAACCAATAAATCAGGGACGAATGCATC
TGAATATGATTCATCATACATATTATCATCATTAAGATCTAATATAATGGCATCAATATGCCATGCGCTTGATGATTCAT
TAAGTGACAAGGAAAAAAATGATATACTGCAGTTTCCTGATAGATTGAAAGGTCTAAAGTATTCACTTGACATATTTATT
TCCACATTCTGTATTGAGAACGTTTTTTTCTCTCAGACGGAAATTGCCGGGATCTGTTTATCTGGTGAACTCATGGGGGA
GAAGGACAAAAACTGTATTTTTGCTGATATCCTTATTTCCGAGATACTGCCACAAATACATGATTCAGACGTTATTCATG
TTAACAAACAAAGAGTGAATGTATCGTTTAAATCAGTTATAGCCATGACTGCATTGTGTTTTGTTGGATATAGCGGATAT
TGTTCATATAATGTGTATAACATCAGACATGGTTCTGTTGATACCAGCCATGCATTTCTGACAGAGCAGATATCAAAATA
TGAAGACAAAGTTAGGTCTAACATGCGGTATTTCCCTTTTAAGCCTGCACTAGATGATAAATATCTATTTTTCAGAGAAT
CTCTTCATAAAACAACAAGATTCGATATCAGTCCTGTCTCATGGCGCGTTACTGAATATAAGAAGAATTTCATGCAAGCT
TCACCATCAGGAAAGCGAGAACTAATTCTGTCGCTCTCATCATCACTTATCAGTTGGGATAAGATGATGAAAGATGAATC
CCTGAGTGACTTAGCAAAATCTCCTGGTATTCATGAGTTACTGAAGATAACCAGACCTCATGATAAAATATCATCAATAG
CTTCACTTGCTGTAGAAAGAGATGAGATACAGAAAAATAATGGAATTGAGAATATTTATGTGTTCAGAAACCTCCTGACT
GAGTTGGTTCAGAGTGACCCGTCTTATTCATGGTTTGTTTCTGAAGATGTTAATATTCCTGCTGTAAGAATTACGGACTT
TTGGGAAGATGAAAACTCTTCTGTCTATCTTTCCGGAATATGGACACAACCGGGACAGAATAAACTTCATCAGTGGTATG
AGACTATCAAAGAGGCATATGGACGTGATACGGTTCCGGAGGCGTTCTCATCCTTTGTGCTATACCTGGATGAAAGTCGT
CAGGAGCATTTCAGACAGTTTATTATGTCTGTTGCAAGGGCACGTAAAGACTCACATTCAGGTTTAATGAATCCGCTGCA
ACTGACCAATATTATACACAATCGCTCATCTGAGCATAGATTCTTTCAGTTTGTAGATGATGAATTACACAATATACCAA
CGAGTTCGGCGCAGGACTGGCTTTCTGAATTCAGACTGCTTAACCATCTGTTTTCACTTAAAGTGGATAACGGAATGAAA
CGTCAAATAGAACAATTTGATCTCATGTTACGTATATACCTGATTTCTGTTCTTAATAACAGTCAAATGAACAGGACACT
GACTCATGTTACCACATGGAGAAGCTGGCAGAATGCTCTACGTAATGCCGTGAATTCAGTGTTACATACAGCATCATCAG
TTGAACTTATTCGTAATGCCATGCGTTCAGATCCAGAAAACAAGCTGGTAATATTATTTGATGAGTTTGAAAAGGTTCGC
TCAGTCATTAACAGCAATAATAGAGAGCCGGTGATTGATTCTGTATGGGATATTTATGAGCGGCAGATTTATCAACTTCT
CGACCATGCTGTCACTTATACAGGGTGTTGGGTTGGTGAACAATGGAGGAATTCTGTTTTAGGCCGGTTTAACTCAGGAA
AACATAATCTCAGTTATTCTGAGATGCAGGGAAAAGTATATAAGGACATTATTGGCTTTTTGAAAGGTCCGTCTAACGGA
GTTTTAGCGCTTGATCCAGACGGTGTCAGACTGTTGTCCTTCAGGGAAAGAAGTATACCTTTTTCCCCTTCATTCATTAC
TTTCATTAATGACATTGTTTCCCCGGATGATCTGCTTGATGTTTGGCTCAGAGAGCGAACGCAAAACAAGGATGAATTAA
TAAATGTACAGGGGCAACTAGACTTACTGAATCAGACCCTACAAAATGCTGAGTCACAGCCTTACAGAGTTACGATAGAT
AGTGCACCTGCGACAATTCCGGATAATCCCCGAGTCAAACCAACAGGTACAACTCTGACTCTTGAGTGTAAAACAGGTAA
CAGCAGTATACGAAGTATGAATTTTGCTGATTCAGGTATTTTTACCTGGTATCCGGGAAGCTGTCACTCTGTAAGAATTG
ACATTTTGTTCCCTAATTTTTCTGCCACCTATAAGTTTACAGGAGAAACAGCCTGGATTGATTTTATCAACAAGTTTTCG
GATGGAGAGAGTGAGTTAATGACAAAGGATTTTTCTCCAGAATCAAGAAATTTTCTCGAATCAATGGGGATTAAAGGCAT
TCTTGTCCGTTACAAACTGAGTGATACAGGCAATCTAAGTCAGGCATATATCGAATGGGAACAACTAAAACAGGAAAAGG
ATAAACTTAAAGATTTACAGGTGAATCTGAGTAATAAGCTGCTTACCACACATAGCTGGGAGAAAAGTGCCTGGATTTCC
AGACTGCCCGGCAATATAACAATATGTCCAGTAGTACAGGAGTGA					