Detailed information of component protein


Summary

Component ID T6CP009058
Component protein type TssA
T6SS ID (Type) T6SS00485 insolico (Type i1)
Strain Escherichia coli 042
Replicon chromosome
Sequence Protein sequence (453 a.a.); Nucleotide sequence (1359 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) EC042_RS01145
Coordinate (Strand) 244026..245384 (-)
NCBI ID WP_000073803.1
RefSeq NC_017626
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 3e-24 288..433
ImpA_N PF06812.14 1.5e-20 12..113
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-235 MSNYALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKLVESLALSIFKANGVDLHTATYYTLARTRNQGLAGFCEGSELLAAMITHEWDKFWPQGGPARTEMLDWFNTRTGNILRQQVSFSENDLPLLYRTERALQLICDKLQQVELKRQPRVENLLYFVQNTRKRFEPQPGNRTDTAAQTTVRTLVYAPESTASTTAEAVPPLPDLPEMRVEVRGVANNADNVRQGST
Transmembrane helix 236-258 VKGFVAGAACTAVIAAVLWWWQA
Outside 259-452 YPIQRQLTQVRDTAQGAATAWLASPVLKEYEQYLQQLLDAPPLQPLETGMQMMRTADTLWPESLQQQEASRMWSNTLRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQETPLEYLLTQYQETRARKQDTQALEKEINERLDGLLSRWLLLKNTGQDMATDTRTEPVHPTH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9740 - -


  Protein sequence: 453 a.a.    

>T6CP009058 NC_017626:c245384-244026 [Escherichia coli 042] [TssA]
MSNYALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKLVESLALSIFKANGVDLHTATYYTLARTRNQGLAGFC
EGSELLAAMITHEWDKFWPQGGPARTEMLDWFNTRTGNILRQQVSFSENDLPLLYRTERALQLICDKLQQVELKRQPRVE
NLLYFVQNTRKRFEPQPGNRTDTAAQTTVRTLVYAPESTASTTAEAVPPLPDLPEMRVEVRGVANNADNVRQGSTVKGFV
AGAACTAVIAAVLWWWQAYPIQRQLTQVRDTAQGAATAWLASPVLKEYEQYLQQLLDAPPLQPLETGMQMMRTADTLWPE
SLQQQEASRMWSNTLRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQETPLEYLLTQ
YQETRARKQDTQALEKEINERLDGLLSRWLLLKNTGQDMATDTRTEPVHPTH*

  Nucleotide sequence: 1359 bp    

>T6CP009058 NC_017626:c245384-244026 [Escherichia coli 042] [TssA]
ATGAGCAATTACGCACTGACACAAACTATCGTTACCGGCAGCGATCCGCGCGCCCTGCCGGAGTTCAGCGCCATCCGCGA
AGAAATTAACAAAGCTAACCACCCGTCACAGCCGGAGCTGAACTGGAAACTGGTGGAATCGCTGGCGCTGTCGATTTTTA
AGGCCAACGGGGTGGATTTGCATACCGCCACCTACTATACGCTGGCGCGTACCCGTAATCAGGGGCTGGCAGGGTTCTGC
GAAGGCTCGGAACTGCTGGCTGCGATGATCACCCACGAATGGGATAAATTCTGGCCGCAGGGTGGCCCGGCGCGGACTGA
GATGCTGGACTGGTTCAATACCCGCACCGGGAATATTCTGCGCCAGCAGGTGTCCTTTTCCGAAAATGATCTGCCGTTGC
TCTACCGCACGGAACGGGCTTTACAGCTTATTTGCGACAAACTCCAGCAGGTGGAACTGAAGCGCCAGCCGCGCGTGGAA
AACCTGCTTTATTTTGTGCAGAACACACGCAAGCGGTTTGAGCCGCAGCCCGGAAACCGTACTGATACGGCGGCACAGAC
CACGGTCCGGACGCTGGTCTATGCGCCGGAAAGCACGGCTTCCACCACAGCGGAAGCCGTGCCTCCCCTGCCCGACCTGC
CGGAAATGCGGGTGGAGGTGCGCGGTGTGGCAAATAACGCCGACAACGTTAGACAGGGCAGCACGGTAAAAGGTTTTGTT
GCCGGTGCCGCCTGTACTGCCGTTATCGCGGCAGTACTCTGGTGGTGGCAGGCGTATCCGATACAACGGCAGCTTACGCA
AGTCAGGGATACCGCACAGGGAGCCGCCACCGCCTGGTTGGCCTCCCCGGTACTGAAAGAATATGAACAGTACCTGCAAC
AGCTTCTGGATGCCCCGCCACTACAGCCGCTGGAAACCGGAATGCAGATGATGCGCACCGCAGACACCCTCTGGCCGGAA
AGCCTGCAACAGCAGGAAGCCAGCAGGATGTGGAGCAACACGCTGAGAAACCGGGCGCAAGCCAGTCCGCAGATGAAGGG
CTGGCAACAGGCGCGGCAGAATTTGCGGGACTTTGCGGATCTGATGATGAAAAAGGAGACGGAGAAACAGGGTTTCACCC
TCTCGTATATCAAGACGGTGACCTGGCAGGCCGAACGGTTACTGAATCAGGAAACACCGCTGGAGTACCTGCTGACACAG
TACCAGGAGACACGCGCACGGAAGCAGGACACACAGGCGCTGGAAAAAGAGATCAACGAACGGCTGGACGGGCTGTTAAG
CCGCTGGCTACTGCTGAAAAATACCGGACAGGATATGGCAACGGATACCAGAACCGAACCTGTCCATCCGACTCATTGA