Detailed information of component protein


Summary

Component ID T6CP008526
Component protein type TssA
T6SS ID (Type) T6SS00157 experimental (Type i1)
Strain Aeromonas hydrophila subsp. hydrophila ATCC 7966
Replicon chromosome
Sequence Protein sequence (477 a.a.); Nucleotide sequence (1431 bp)
Reference
[1] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190
[2] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[3] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[4] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[5] Khajanchi BK, Sha J, Kozlova EV, et al. N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology (Reading). 2009 Nov;155(Pt 11):3518-3531. doi: 10.1099/mic.0.031575-0. Epub 2009 Sep 3. PMID: 19729404
[6] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[7] Wang N, Wu Y, Pang M, et al. Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015 Oct;46(2):297-304. doi: 10.1016/j.fsi.2015.06.019. Epub 2015 Jun 18. PMID: 26093203
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AHA_RS09300
Coordinate (Strand) 2017683..2019113 (+)
NCBI ID 500025008
RefSeq NC_008570
Uniprot ID A0KJC9_AERHH
KEGG ID aha:AHA_1846
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 3.3e-54 324..470
ImpA_N PF06812.14 5.8e-23 10..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-249 MSQNQQGQQALKVGRDPRMLPEYEALRAEINKLSHASRPEVDWQRIHQLASLIFEKHGVDLQTAIYFTLARSRLQGLSGFTEGCEFLANLIVTQWESFWPPVHQERARIEMLDWFIARISDVIRQYQISHEDKRLIYRCERALQLISEKLHNADLSRIPRVENLVHFIEGYTHLFDETEIVIVSDEPGLKEDLQIPPMVFFKSDMESDHGSTVSASTPHLPQGSILVGCEKGQVKPTVLKIEQHRRQRP
Transmembrane helix 250-272 AWFWFGCGLLSCALPVMGWLGWQ
Outside 273-476 QHQQEKTVAAQRLVQPAAELPRALSYDDIRQARIVLGEQTLQNMESDLVARYQNQLTRLEQTSPLYWYRYGEGLRNSLQMLYPDSLAVKALDKQWQAQLGGLQGDVISVPTYLDARAGVDALLDQLLELERQRKTVTISYLKSQLYEVQKNLMQNIPFSLRLGELEARKASQEPITAAELKSLENDLKALNIRLYQLQQGASGS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9958 - -


  Protein sequence: 477 a.a.    

>T6CP008526 NC_008570:2017683-2019113 [Aeromonas hydrophila subsp. hydrophila ATCC 7966] [TssA]
MSQNQQGQQALKVGRDPRMLPEYEALRAEINKLSHASRPEVDWQRIHQLASLIFEKHGVDLQTAIYFTLARSRLQGLSGF
TEGCEFLANLIVTQWESFWPPVHQERARIEMLDWFIARISDVIRQYQISHEDKRLIYRCERALQLISEKLHNADLSRIPR
VENLVHFIEGYTHLFDETEIVIVSDEPGLKEDLQIPPMVFFKSDMESDHGSTVSASTPHLPQGSILVGCEKGQVKPTVLK
IEQHRRQRPAWFWFGCGLLSCALPVMGWLGWQQHQQEKTVAAQRLVQPAAELPRALSYDDIRQARIVLGEQTLQNMESDL
VARYQNQLTRLEQTSPLYWYRYGEGLRNSLQMLYPDSLAVKALDKQWQAQLGGLQGDVISVPTYLDARAGVDALLDQLLE
LERQRKTVTISYLKSQLYEVQKNLMQNIPFSLRLGELEARKASQEPITAAELKSLENDLKALNIRLYQLQQGASGS*

  Nucleotide sequence: 1431 bp    

>T6CP008526 NC_008570:2017683-2019113 [Aeromonas hydrophila subsp. hydrophila ATCC 7966] [TssA]
ATGTCACAGAATCAACAAGGTCAGCAGGCCCTGAAAGTGGGGCGCGACCCCAGGATGTTGCCGGAGTACGAGGCGCTGCG
AGCCGAGATCAACAAGCTCAGCCACGCTTCGCGCCCCGAGGTGGACTGGCAGCGGATCCATCAGCTCGCCAGCCTTATCT
TCGAGAAACACGGGGTGGATCTGCAGACCGCCATCTATTTCACCCTGGCGCGCTCGCGCCTGCAGGGGCTCAGCGGTTTT
ACCGAAGGGTGCGAGTTTCTCGCCAACCTCATCGTCACCCAGTGGGAGAGCTTCTGGCCGCCGGTCCATCAGGAGCGGGC
CCGCATCGAGATGCTCGACTGGTTCATCGCCCGCATCAGCGACGTCATTCGCCAGTACCAGATCAGCCACGAGGACAAGC
GGCTGATCTATCGCTGCGAGCGGGCGCTGCAGCTCATCAGCGAGAAGCTGCACAACGCGGATCTGAGCCGCATTCCCCGG
GTCGAGAATCTGGTGCACTTCATCGAGGGGTATACCCACCTGTTCGATGAGACCGAGATCGTCATCGTCTCCGACGAGCC
GGGCCTCAAGGAGGATCTGCAGATCCCGCCCATGGTCTTCTTCAAGTCAGACATGGAGAGTGATCACGGCAGCACGGTTT
CGGCATCGACCCCGCACCTGCCCCAGGGCAGCATTCTGGTGGGGTGCGAGAAGGGGCAGGTCAAGCCCACCGTGCTCAAG
ATTGAGCAGCACCGCCGGCAGCGTCCGGCCTGGTTCTGGTTTGGCTGCGGCCTGCTCAGCTGCGCCCTGCCGGTGATGGG
CTGGCTGGGCTGGCAGCAACATCAGCAGGAGAAAACCGTGGCGGCCCAGCGTCTGGTGCAGCCGGCCGCCGAGCTGCCCC
GAGCCCTCAGCTATGACGACATTCGCCAGGCGCGCATCGTGCTGGGGGAGCAGACCCTGCAGAACATGGAGAGCGATCTG
GTGGCCCGCTACCAGAACCAGTTGACCCGGCTCGAGCAGACGTCGCCGCTCTACTGGTATCGCTACGGCGAGGGGCTGCG
CAACTCGTTACAGATGCTCTATCCCGACTCGCTGGCGGTCAAGGCGCTGGACAAGCAGTGGCAGGCGCAGCTTGGTGGCC
TGCAGGGGGATGTCATCAGCGTACCCACCTACCTGGATGCCCGCGCCGGGGTGGATGCCCTGCTCGATCAGCTGCTGGAG
CTGGAGCGTCAGCGCAAGACGGTGACCATCTCCTACCTCAAATCCCAGCTCTACGAAGTACAGAAGAACCTGATGCAGAA
CATCCCGTTCAGTCTGCGTCTCGGTGAACTGGAGGCGAGAAAAGCCTCCCAGGAGCCCATCACGGCTGCCGAGCTCAAAA
GCCTGGAAAATGATTTGAAAGCGCTCAATATCCGCCTCTATCAGCTGCAACAGGGCGCTTCCGGTAGCTGA