SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP008436
Component protein type	TssA
T6SS ID (Type)	T6SS00053 (Type Unknown)
Strain	Photorhabdus luminescens subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (533 a.a.); Nucleotide sequence (1599 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS20785
Coordinate (Strand)	4916971..4918569 (-)
NCBI ID	WP_011148307.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasJ	PF16989.7	1.2e-84	229..489
ImpA_N	PF06812.14	2.8e-19	31..135

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-532	MLQQLIESCFVERDVLAEARQNAARWENWLLPISTDAPTGNDPVYEDDFQCMREEVNKISGVDTSLICQLAEKLLTTKCKDVRVATYYVWARLHCEGEHGLAEGLALLAGLADRFGEALLPSRASSRKAALEWLTGAKMRDSLSRYPEVNRTDFDHILAALVLLEQSFSSWDEESRPSLVGLARTLENRLAQSGGVSAVVPQTAAPVLPSAGQSKASASGKSPVWRQVQSGRDLLDQARELVRYLRDQPQGWLSSARLIRSIRWDTVHQLPPQDADERTRLVPPRTESRAQLKRLYRQQNWHELLEQAERMFAEGVNHFWLDLQWYLCQALSRLGCPYEGWADIMKHDLRILLERLPGLESMAYNDGTPFADEVTTSWIAQQVHDNQSDWQFEHLPAQPRHDDDVLALEAEALQLADSEGIDAALRWLGQLPGINGARHRWLQRLLMARVAEQYGKNEMALHLLTELDADEQFNLGEWEPELMFEVKACLLKLLRMKAQRSEHDKARMAQRMETLLAGLVAIDPVRASVLCG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9869	-	-

Protein sequence: 533 a.a.

						>T6CP008436 NC_005126:c4918569-4916971 [Photorhabdus laumondii subsp. laumondii TTO1] [TssA]
MLQQLIESCFVERDVLAEARQNAARWENWLLPISTDAPTGNDPVYEDDFQCMREEVNKISGVDTSLICQLAEKLLTTKCK
DVRVATYYVWARLHCEGEHGLAEGLALLAGLADRFGEALLPSRASSRKAALEWLTGAKMRDSLSRYPEVNRTDFDHILAA
LVLLEQSFSSWDEESRPSLVGLARTLENRLAQSGGVSAVVPQTAAPVLPSAGQSKASASGKSPVWRQVQSGRDLLDQARE
LVRYLRDQPQGWLSSARLIRSIRWDTVHQLPPQDADERTRLVPPRTESRAQLKRLYRQQNWHELLEQAERMFAEGVNHFW
LDLQWYLCQALSRLGCPYEGWADIMKHDLRILLERLPGLESMAYNDGTPFADEVTTSWIAQQVHDNQSDWQFEHLPAQPR
HDDDVLALEAEALQLADSEGIDAALRWLGQLPGINGARHRWLQRLLMARVAEQYGKNEMALHLLTELDADEQFNLGEWEP
ELMFEVKACLLKLLRMKAQRSEHDKARMAQRMETLLAGLVAIDPVRASVLCG*					

Nucleotide sequence: 1599 bp

						>T6CP008436 NC_005126:c4918569-4916971 [Photorhabdus laumondii subsp. laumondii TTO1] [TssA]
ATGCTGCAACAGCTAATTGAGAGTTGTTTTGTTGAACGTGATGTCCTTGCGGAAGCACGGCAGAACGCGGCGCGGTGGGA
AAACTGGTTGTTACCCATTAGTACCGATGCGCCTACAGGTAATGATCCGGTCTATGAAGACGATTTTCAGTGTATGCGGG
AGGAGGTTAATAAAATCAGTGGTGTTGATACTTCCCTGATTTGTCAACTGGCGGAGAAGTTGTTGACCACCAAATGCAAA
GATGTCCGCGTGGCGACCTACTATGTGTGGGCCCGTCTGCATTGTGAGGGGGAACATGGTCTGGCAGAAGGGTTGGCGCT
ATTAGCCGGATTGGCGGATCGGTTTGGTGAAGCCCTGTTACCCAGTCGGGCTAGCAGCCGGAAAGCGGCGCTGGAGTGGC
TGACAGGTGCAAAAATGCGGGACAGTTTATCACGCTATCCAGAGGTAAATCGGACCGATTTTGACCACATTCTGGCCGCG
TTGGTGTTACTGGAACAGTCATTTTCCTCTTGGGATGAAGAATCTCGTCCAAGCTTGGTCGGGCTGGCAAGAACACTGGA
GAACCGATTAGCTCAGTCCGGTGGCGTGAGTGCGGTAGTGCCACAAACTGCCGCTCCGGTACTGCCATCGGCGGGACAGA
GCAAGGCATCAGCATCGGGTAAGTCACCGGTATGGCGTCAGGTGCAATCTGGGCGTGATCTGCTGGATCAGGCGCGTGAA
CTGGTGCGTTATCTGCGTGATCAACCTCAGGGCTGGCTCTCTTCCGCCCGGCTGATACGTAGTATCCGTTGGGATACCGT
CCATCAACTACCACCACAGGATGCTGATGAGCGTACCCGTCTGGTGCCTCCCCGCACTGAATCTCGCGCTCAGTTAAAGC
GGCTCTACCGGCAACAGAACTGGCATGAGTTATTGGAACAGGCCGAGCGCATGTTTGCCGAAGGGGTGAATCATTTTTGG
CTGGATTTGCAATGGTACCTCTGTCAGGCATTAAGCCGATTGGGCTGCCCTTACGAAGGTTGGGCGGACATCATGAAACA
CGATCTGAGAATACTGCTTGAGCGGCTGCCGGGGCTGGAGAGTATGGCCTATAACGATGGGACGCCCTTTGCTGATGAGG
TGACAACCAGTTGGATAGCCCAACAGGTGCATGACAATCAGTCAGATTGGCAGTTTGAGCATTTGCCGGCGCAACCTCGA
CATGATGATGACGTTTTGGCGCTGGAAGCGGAGGCGCTGCAACTGGCGGACAGCGAAGGCATTGATGCGGCGTTGCGTTG
GTTGGGGCAATTGCCCGGTATTAATGGAGCCCGTCACCGCTGGTTACAGCGCTTGCTGATGGCGCGGGTCGCTGAGCAAT
ACGGCAAAAATGAAATGGCGTTGCATTTGTTGACTGAGCTTGATGCCGATGAGCAATTCAATCTTGGCGAATGGGAGCCG
GAACTGATGTTTGAGGTGAAAGCATGTCTGCTTAAGTTACTGCGCATGAAAGCTCAACGCAGTGAACATGATAAAGCGAG
GATGGCGCAACGGATGGAGACGCTGCTGGCTGGATTGGTGGCGATTGATCCGGTCAGAGCTTCGGTGCTTTGCGGTTAA					