SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP008432
Component protein type	TssE
T6SS ID (Type)	T6SS00050 (Type i1)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (147 a.a.); Nucleotide sequence (441 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS01800
Coordinate (Strand)	398357..398797 (-)
NCBI ID	WP_011144759.1
RefSeq	NC_005126
Uniprot ID	A0A6L9JJG1_PHOLM, Q7N9G6_PHOLL
KEGG ID	plu:plu0370, plum:A4R40_01805
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	3.6e-18	34..121

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-146	MAALHSWNREGSASLFERIQGKNSGSSRQSRMRELLNSIKRHLNHVLNTHPGACQSAADLGVIDLNDATTTATDFKQSIEQAIEHCITNYEPRISSVTVNAIHDDSDPLLLRFHISAQVSLDDIHDAVEFNIQLDNNRRYCLERTQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9727	-	-

Protein sequence: 147 a.a.

						>T6CP008432 NC_005126:c398797-398357 [Photorhabdus laumondii subsp. laumondii TTO1] [TssE]
MAALHSWNREGSASLFERIQGKNSGSSRQSRMRELLNSIKRHLNHVLNTHPGACQSAADLGVIDLNDATTTATDFKQSIE
QAIEHCITNYEPRISSVTVNAIHDDSDPLLLRFHISAQVSLDDIHDAVEFNIQLDNNRRYCLERTQ*					

Nucleotide sequence: 441 bp

						>T6CP008432 NC_005126:c398797-398357 [Photorhabdus laumondii subsp. laumondii TTO1] [TssE]
ATGGCTGCGCTGCACAGTTGGAATAGAGAAGGCTCTGCCAGCCTGTTCGAGCGTATTCAGGGGAAGAACTCTGGTTCTTC
CCGCCAATCAAGAATGCGTGAGCTGCTCAACTCCATAAAACGGCACCTGAATCATGTTCTCAATACTCATCCGGGTGCCT
GTCAGAGTGCGGCTGATCTTGGCGTCATTGATCTCAATGACGCTACAACCACGGCAACTGACTTTAAACAGAGTATTGAG
CAAGCAATAGAGCACTGCATCACAAATTATGAACCAAGAATATCGTCAGTCACTGTCAATGCAATCCACGATGATAGTGA
TCCGCTGTTGTTACGTTTTCACATCAGCGCCCAGGTATCCCTGGATGATATTCATGACGCTGTTGAATTTAATATTCAAC
TGGATAACAACCGGCGTTATTGCCTAGAGAGGACTCAATAA					