SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP008413
Component protein type	TssA
T6SS ID (Type)	T6SS00019 (Type i2)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (534 a.a.); Nucleotide sequence (1602 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS08390
Coordinate (Strand)	1681348..1682949 (+)
NCBI ID	WP_002211937.1
RefSeq	NC_003143
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasJ	PF16989.7	7.3e-80	230..490
ImpA_N	PF06812.14	1.9e-20	33..138

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-533	MATLTTLLTACAAEPEPLLQQARQQVALWERWLQPVTPDKHTGEDPGYDDHFQQMREEVNKLSGADTTLTCELAEKLFTTHGKDVRVATYYVWARLHRDGEAGLADGLSLLAGLITRFGEGLHPLRTTSRKTALEWLAGSRMRDSLSLYPEVDKADFERIVGALALIEQRLSLWDEGVRPQLGGLYTALENRLAQSGGLNAVVPQNSSGSSSAGSLNPANTASPALRPVQSGRDLLDQTKTLAKYLRNQPQGWLSGHHLIKSVRWDTVHQSPPLDVNGRTRLVPPRPEYRVQLKRLYLQQNWLALLEQAESIFAEGVNHFWLDVQWYLHQALSKAGAPFDGWASCITQDLRLLLTRLPGLEGLCWSDGTPFADEVTLGWINQQVLESVSGWGSEPAAVSSGEDGILLLEPEALAQADSEGIEAALNWLQSRPGITTARHQWLLRLVMARVAEQYGKNDMALHLLSGLDSSGALLTLPQWEPGLVFEVKARRLKLLRMKAQRGDSDKTRLHAEMESLLSGLIALDPARAAVLCG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.4714	pos: 10-11. LTA-CA.	MATLTTLLTA

Protein sequence: 534 a.a.

						>T6CP008413 NC_003143:1681348-1682949 [Yersinia pestis CO92] [TssA]
MATLTTLLTACAAEPEPLLQQARQQVALWERWLQPVTPDKHTGEDPGYDDHFQQMREEVNKLSGADTTLTCELAEKLFTT
HGKDVRVATYYVWARLHRDGEAGLADGLSLLAGLITRFGEGLHPLRTTSRKTALEWLAGSRMRDSLSLYPEVDKADFERI
VGALALIEQRLSLWDEGVRPQLGGLYTALENRLAQSGGLNAVVPQNSSGSSSAGSLNPANTASPALRPVQSGRDLLDQTK
TLAKYLRNQPQGWLSGHHLIKSVRWDTVHQSPPLDVNGRTRLVPPRPEYRVQLKRLYLQQNWLALLEQAESIFAEGVNHF
WLDVQWYLHQALSKAGAPFDGWASCITQDLRLLLTRLPGLEGLCWSDGTPFADEVTLGWINQQVLESVSGWGSEPAAVSS
GEDGILLLEPEALAQADSEGIEAALNWLQSRPGITTARHQWLLRLVMARVAEQYGKNDMALHLLSGLDSSGALLTLPQWE
PGLVFEVKARRLKLLRMKAQRGDSDKTRLHAEMESLLSGLIALDPARAAVLCG*					

Nucleotide sequence: 1602 bp

						>T6CP008413 NC_003143:1681348-1682949 [Yersinia pestis CO92] [TssA]
ATGGCGACGTTAACTACCTTGCTCACCGCTTGTGCCGCAGAGCCTGAGCCACTTTTACAGCAGGCCAGACAACAGGTTGC
GCTCTGGGAACGTTGGTTGCAACCGGTGACACCCGATAAGCATACCGGTGAAGACCCCGGTTACGATGACCACTTCCAAC
AGATGCGTGAAGAGGTCAATAAGCTCTCGGGTGCCGATACCACGCTGACCTGCGAGCTGGCTGAAAAGCTGTTCACCACA
CACGGCAAGGATGTGCGGGTGGCAACCTATTACGTCTGGGCGCGGTTGCACCGTGACGGCGAGGCCGGGCTGGCGGATGG
CCTTTCCCTGCTGGCCGGGCTGATCACGCGCTTTGGTGAGGGCCTGCATCCGCTGCGTACAACCAGCCGGAAAACCGCGT
TGGAGTGGCTGGCCGGCTCCCGGATGCGCGACAGCTTGTCACTTTACCCGGAAGTGGATAAAGCCGATTTTGAGCGTATC
GTGGGCGCACTGGCCCTTATCGAACAGAGGCTCAGTCTCTGGGACGAAGGCGTCCGGCCGCAACTGGGCGGGTTGTATAC
CGCCCTGGAAAACCGCCTCGCGCAATCTGGTGGGCTAAATGCCGTGGTGCCGCAAAACAGCAGCGGGTCATCATCGGCAG
GCAGCTTGAACCCAGCCAACACGGCGTCACCGGCGTTGCGGCCAGTGCAATCGGGACGTGACCTGCTCGATCAGACCAAA
ACGCTGGCAAAATACCTGCGCAACCAGCCGCAGGGCTGGCTCTCCGGGCATCATCTTATCAAAAGCGTGCGCTGGGATAC
GGTACATCAGTCACCGCCATTAGATGTGAACGGACGGACCCGGCTGGTACCGCCGCGCCCGGAATATCGCGTACAACTCA
AGCGCCTTTATCTGCAACAAAACTGGCTGGCGTTGCTGGAACAGGCCGAATCGATATTTGCCGAAGGGGTTAACCATTTC
TGGCTCGATGTGCAGTGGTATCTGCATCAGGCCCTCAGCAAAGCGGGGGCCCCATTCGATGGCTGGGCGAGCTGTATCAC
GCAGGATTTACGGTTATTACTGACCCGGCTGCCGGGGCTGGAAGGGCTATGCTGGAGTGACGGCACGCCATTTGCCGATG
AGGTGACGCTGGGGTGGATAAACCAGCAGGTACTGGAGTCGGTCTCGGGCTGGGGCAGCGAGCCGGCCGCCGTTTCATCC
GGTGAGGATGGCATTCTGTTGCTGGAGCCAGAAGCTTTGGCTCAGGCGGACAGCGAAGGAATTGAAGCGGCGCTTAACTG
GTTGCAGTCACGACCGGGGATCACCACGGCGCGCCATCAGTGGCTGCTGCGTCTGGTGATGGCCCGGGTGGCGGAGCAGT
ACGGTAAGAACGATATGGCCTTGCATCTGCTGAGTGGCCTTGATAGCAGCGGCGCGTTGTTGACCTTGCCACAGTGGGAG
CCGGGGTTGGTGTTTGAAGTTAAAGCCCGCCGTTTGAAGCTGTTGCGTATGAAGGCACAGCGGGGTGACAGTGATAAAAC
CCGCCTGCATGCTGAAATGGAAAGCCTGCTCAGTGGGCTTATCGCCCTCGACCCGGCCCGTGCGGCCGTATTATGCGGAT
AA					