SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP008410
Component protein type	TssM
T6SS ID (Type)	T6SS00017 (Type i3)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (1147 a.a.); Nucleotide sequence (3441 bp)
Reference	[1] Robinson JB, Telepnev MV, Zudina IV, et al. Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models. Microb Pathog. 2009 Nov;47(5):243-51. doi: 10.1016/j.micpath.2009.08.005. Epub 2009 Aug 27. PMID: 19716410 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Cathelyn JS, Crosby SD, Lathem WW, et al. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. Epub 2006 Aug 28. PMID: 16938880

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS03585
Coordinate (Strand)	552875..556315 (+)
NCBI ID	WP_002214734.1
RefSeq	NC_003143
Uniprot ID	UPI00006DD26E
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	1e-60	494..775
IcmF-related_N	PF14331.8	2.6e-29	179..359
IcmF_C	PF06744.14	5.8e-20	1023..1124

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-22	VVDRAADRHWPLFPSGGVWVRK
Transmembrane helix	23-45	IIIALILFWALWPFVAIFFSWIF
Inside	46-415	RYARAPLPKRKKKTVQLDRVSARFHDAMLTLQHADLSSQKTRWQRWRQRRKRQYIDAKPWFLVMGPPGCGKTSIVYESSEKFLLSEQYGLAQTTDIGPTQDCNWWLTDRAVYIDTAGEWILLHGQSEEAGTARQKLFSMIRKYRRYPGVDGIVLCLDVSVLLNSSVTERKSLADTLRLRILEVASTFQADIAVYLFLNNIDKLPGGETFLSLMNDELLAGGLGISMTHNEKGQNNFVADEERYRELLTRVSRYVLEILHDSPNMEMRHQLLLFTESLGALQKPLFGLFEQIFPATPIGYTGCLRQFWFGSTQNLTSWEALYYPSSTSTFESRPAGQMYFPALSQAITERGVLHATGPMPLRNRLLNIGKY
Transmembrane helix	416-435	VFVVLVLCILLLMLSARYFW
Outside	436-1146	EYDYIGYATARFEETRRIVRDIPITNQVNDNLVAAYEQLGYINTHYLESSPPLLTPYFEHRLLNQAMLQTYHRHLNKVFWPAVQNYVTNELKKDTQSPDGDNYDTLKVYLMLGQPDHRSQEALVQWFMSRWESFAPQGYGQNDRKLFSYHLSELFSDTASAAPKVKLNDELIRESRIKAMKTPIHIRVINRIHDAPLPARIEDLSLADAAGPSVTLMLRRKSPSTVTDLAIPAFYTRASYHDVVNPQLQEASEAMINEEAWVLSDGKASVSQVSLLAAAQKLSDEARKYYLMEYANQWDAFFNDIRVRPINGLDDAALLARQLSDPSSPLANLVRFSTREITLTTDNNGKGAMSSGWFNIQRNKLTTARRNIIDEMSGERSRFRLTPEKAVEDRFELLRRLGYALQSASTSNSDPLGRTFEQIYNQLITLSTSLRAGQILPQNNDFSRLQIDMARQPEPVRSIMMDLLAVGQTQSLQQSKANLSKGVSSIASDLCSNAVSGRYPFNRLASEEIGIGDFNRMFGRSGAMQSFFDQNLAPYVDTNGNRWKVKPDSNGVVTPKTLRSFENSAQIRDTFFDASGKMAMSMIIRPISLTPSILEAVLDVDGQIINYSHGSNQPVKVDWPGPKGGVYIRLTFKTQDGNVETVSFNGPWAIFRMYDVSNPRPLGVNSRELMMVMDNVSGVFKIELSSTMKDYPLWSKALAQFSCPKNL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9981	-	-

Protein sequence: 1147 a.a.

						>T6CP008410 NC_003143:552875-556315 [Yersinia pestis CO92] [TssM]
VVDRAADRHWPLFPSGGVWVRKIIIALILFWALWPFVAIFFSWIFRYARAPLPKRKKKTVQLDRVSARFHDAMLTLQHAD
LSSQKTRWQRWRQRRKRQYIDAKPWFLVMGPPGCGKTSIVYESSEKFLLSEQYGLAQTTDIGPTQDCNWWLTDRAVYIDT
AGEWILLHGQSEEAGTARQKLFSMIRKYRRYPGVDGIVLCLDVSVLLNSSVTERKSLADTLRLRILEVASTFQADIAVYL
FLNNIDKLPGGETFLSLMNDELLAGGLGISMTHNEKGQNNFVADEERYRELLTRVSRYVLEILHDSPNMEMRHQLLLFTE
SLGALQKPLFGLFEQIFPATPIGYTGCLRQFWFGSTQNLTSWEALYYPSSTSTFESRPAGQMYFPALSQAITERGVLHAT
GPMPLRNRLLNIGKYVFVVLVLCILLLMLSARYFWEYDYIGYATARFEETRRIVRDIPITNQVNDNLVAAYEQLGYINTH
YLESSPPLLTPYFEHRLLNQAMLQTYHRHLNKVFWPAVQNYVTNELKKDTQSPDGDNYDTLKVYLMLGQPDHRSQEALVQ
WFMSRWESFAPQGYGQNDRKLFSYHLSELFSDTASAAPKVKLNDELIRESRIKAMKTPIHIRVINRIHDAPLPARIEDLS
LADAAGPSVTLMLRRKSPSTVTDLAIPAFYTRASYHDVVNPQLQEASEAMINEEAWVLSDGKASVSQVSLLAAAQKLSDE
ARKYYLMEYANQWDAFFNDIRVRPINGLDDAALLARQLSDPSSPLANLVRFSTREITLTTDNNGKGAMSSGWFNIQRNKL
TTARRNIIDEMSGERSRFRLTPEKAVEDRFELLRRLGYALQSASTSNSDPLGRTFEQIYNQLITLSTSLRAGQILPQNND
FSRLQIDMARQPEPVRSIMMDLLAVGQTQSLQQSKANLSKGVSSIASDLCSNAVSGRYPFNRLASEEIGIGDFNRMFGRS
GAMQSFFDQNLAPYVDTNGNRWKVKPDSNGVVTPKTLRSFENSAQIRDTFFDASGKMAMSMIIRPISLTPSILEAVLDVD
GQIINYSHGSNQPVKVDWPGPKGGVYIRLTFKTQDGNVETVSFNGPWAIFRMYDVSNPRPLGVNSRELMMVMDNVSGVFK
IELSSTMKDYPLWSKALAQFSCPKNL*					

Nucleotide sequence: 3441 bp

						>T6CP008410 NC_003143:552875-556315 [Yersinia pestis CO92] [TssM]
GTGGTGGATAGGGCCGCTGATCGCCATTGGCCCCTATTTCCCTCTGGGGGGGTATGGGTTCGTAAGATCATTATTGCTCT
GATTTTATTTTGGGCACTGTGGCCATTTGTTGCAATTTTCTTCAGTTGGATTTTTCGTTATGCCCGTGCGCCACTTCCCA
AGCGCAAGAAAAAAACGGTTCAGCTTGATCGCGTTTCTGCACGTTTTCATGATGCGATGCTCACCCTGCAACATGCGGAT
CTTTCAAGCCAGAAAACACGCTGGCAGCGCTGGCGTCAACGGCGTAAGCGCCAGTATATCGATGCTAAACCGTGGTTTCT
GGTCATGGGGCCACCCGGATGCGGTAAAACGTCGATAGTTTATGAGAGTAGCGAGAAGTTCCTACTCTCAGAACAGTATG
GTTTAGCGCAGACCACGGATATTGGACCGACGCAGGACTGTAATTGGTGGCTGACGGATCGTGCTGTTTACATCGATACG
GCAGGGGAATGGATCCTGCTGCATGGTCAGAGCGAAGAAGCGGGCACGGCTCGGCAAAAATTGTTCTCTATGATCCGCAA
ATATCGCCGTTATCCGGGGGTTGATGGGATTGTTCTTTGCCTTGACGTCAGCGTGCTTTTGAACTCTTCGGTGACGGAAC
GTAAGTCACTGGCTGATACGTTGCGCCTGCGGATCCTGGAAGTTGCCTCGACATTTCAGGCCGATATTGCGGTGTACTTG
TTCCTCAACAATATCGATAAGTTACCTGGCGGCGAAACCTTCCTCTCGTTAATGAACGACGAACTTCTGGCCGGAGGGCT
TGGGATTAGCATGACCCATAATGAAAAGGGGCAGAACAATTTCGTTGCCGATGAAGAACGTTACCGTGAACTCCTGACAC
GTGTTAGCCGTTATGTGTTGGAAATACTGCACGATTCGCCCAACATGGAGATGCGCCACCAATTGCTGCTGTTTACAGAA
AGCCTGGGAGCGCTACAAAAACCCCTGTTTGGCTTATTCGAACAAATATTTCCGGCGACACCGATTGGCTATACCGGTTG
TTTGCGGCAGTTCTGGTTTGGCAGTACCCAAAATCTGACCTCATGGGAGGCGCTGTATTATCCGTCATCGACCAGCACCT
TTGAAAGCCGGCCTGCTGGGCAGATGTATTTCCCTGCACTGAGTCAAGCGATCACCGAAAGGGGGGTATTACACGCCACT
GGCCCCATGCCTTTGCGAAATCGCCTGTTAAACATCGGTAAATATGTGTTCGTGGTCCTTGTGCTCTGCATCTTATTGTT
GATGCTGAGCGCCCGCTATTTTTGGGAATATGACTATATTGGCTATGCGACAGCTCGGTTTGAGGAAACCCGTCGGATTG
TGCGTGACATCCCAATCACGAATCAGGTCAACGATAATCTGGTCGCAGCTTACGAACAGTTGGGGTATATCAACACCCAT
TATCTGGAAAGTTCACCGCCACTGCTGACACCGTACTTCGAGCACCGTTTACTGAATCAGGCGATGTTGCAAACCTACCA
TCGTCACCTGAATAAAGTGTTCTGGCCTGCGGTACAAAACTACGTCACCAACGAGCTGAAAAAGGACACTCAATCTCCTG
ACGGTGACAACTATGACACGCTGAAAGTTTACCTGATGCTCGGACAACCTGATCACCGCTCTCAGGAGGCATTGGTGCAA
TGGTTTATGTCGCGCTGGGAAAGTTTTGCGCCTCAGGGATATGGGCAAAACGACCGCAAACTCTTCAGTTATCATTTGAG
TGAACTGTTTTCTGACACCGCCTCAGCTGCCCCGAAAGTTAAGCTGAATGATGAGCTGATCCGTGAATCACGCATTAAAG
CGATGAAAACACCGATACACATCCGGGTGATTAATCGTATTCATGACGCCCCACTGCCCGCGAGGATTGAGGATCTTAGT
CTGGCGGATGCCGCCGGTCCTAGCGTAACACTGATGCTGAGACGTAAAAGCCCCAGTACAGTAACGGATTTGGCGATCCC
GGCTTTCTACACTCGGGCCAGCTACCACGATGTGGTTAACCCACAACTTCAGGAAGCATCGGAAGCGATGATTAACGAGG
AAGCGTGGGTGTTGAGTGACGGTAAAGCTTCGGTTAGTCAGGTTAGTTTACTGGCTGCGGCGCAAAAACTGTCCGATGAA
GCGCGCAAGTATTATCTGATGGAGTATGCCAACCAATGGGATGCATTCTTCAATGATATCAGGGTGCGCCCAATCAACGG
ACTTGATGATGCGGCGCTGCTGGCAAGGCAACTCTCAGACCCGTCATCCCCGTTGGCTAATCTGGTGCGTTTCTCCACAC
GGGAAATAACCCTGACCACTGACAACAATGGTAAAGGGGCGATGTCGTCAGGCTGGTTCAATATTCAACGTAATAAGCTC
ACGACTGCCCGCCGTAACATCATTGACGAAATGTCGGGTGAGCGTTCTCGTTTTCGTCTAACGCCAGAGAAAGCGGTGGA
GGATCGCTTTGAACTATTAAGACGTCTGGGATATGCCCTGCAAAGTGCGTCTACCAGCAATTCTGATCCGTTGGGCCGAA
CCTTCGAGCAGATTTATAACCAGTTGATAACACTCTCGACTTCCCTTCGGGCTGGGCAGATTTTGCCGCAAAATAACGAT
TTCAGTCGCTTGCAAATCGATATGGCCCGTCAGCCAGAGCCGGTGCGCAGCATTATGATGGATCTGCTGGCCGTCGGACA
AACGCAAAGTCTGCAACAAAGTAAGGCCAACCTCAGCAAGGGCGTCTCTTCAATTGCTTCTGATTTATGTAGCAACGCCG
TCAGTGGCCGTTATCCCTTTAATCGGTTGGCTAGCGAAGAGATAGGTATTGGCGATTTCAACCGTATGTTTGGTCGTTCT
GGGGCGATGCAAAGCTTCTTTGATCAGAATCTCGCACCGTATGTCGATACCAACGGCAACCGCTGGAAGGTAAAACCTGA
CAGTAACGGTGTGGTGACCCCTAAAACTCTGCGTTCGTTTGAAAACTCAGCACAAATCCGCGACACCTTCTTTGATGCCT
CCGGCAAGATGGCGATGTCGATGATCATTCGCCCTATCTCACTGACTCCATCAATTTTGGAAGCGGTTCTTGATGTCGAT
GGCCAGATTATTAACTACAGCCATGGTTCTAACCAACCGGTAAAAGTTGATTGGCCAGGACCGAAAGGTGGGGTGTATAT
CCGCCTGACGTTCAAAACTCAGGATGGAAATGTGGAAACCGTTAGCTTCAATGGTCCGTGGGCTATTTTCCGCATGTATG
ACGTTAGTAACCCTAGGCCACTCGGGGTCAATAGCCGTGAACTCATGATGGTGATGGATAACGTGAGTGGCGTCTTCAAA
ATTGAGTTGAGTTCCACCATGAAAGATTATCCGCTGTGGTCAAAAGCATTGGCACAGTTCTCATGTCCTAAGAATCTCTA
A					