SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP008406
Component protein type	TssM
T6SS ID (Type)	T6SS00047 (Type i1)
Strain	Helicobacter hepaticus ATCC 51449
Replicon	chromosome
Sequence	Protein sequence (1185 a.a.); Nucleotide sequence (3555 bp)
Reference	[1] Bartonickova L, Sterzenbach T, Nell S, et al. Hcp and VgrG1 are secreted components of the Helicobacter hepaticus type VI secretion system and VgrG1 increases the bacterial colitogenic potential. Cell Microbiol. 2013 Jun;15(6):992-1011. doi: 10.1111/cmi.12094. Epub 2013 Jan 17. PMID: 23278999 [2] Chow J, Mazmanian SK. A pathobiont of the microbiota balances host colonization and intestinal inflammation. Cell Host Microbe. 2010 Apr 22;7(4):265-276. doi: 10.1016/j.chom.2010.03.004. PMID: 20413095 [3] Ge Z, Sterzenbach T, Whary MT, et al. Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn mice. Microbes Infect. 2008 Jun;10(7):726-33. doi: 10.1016/j.micinf.2008.03.011. Epub 2008 Apr 4. PMID: 18538610

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HH_RS01250
Coordinate (Strand)	243237..246791 (+)
NCBI ID	WP_011115096.1
RefSeq	NC_004917
Uniprot ID	Q7VJJ1_HELHP
KEGG ID	hhe:HH_0252
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	3.4e-40	215..452
IcmF-related	PF06761.14	2.4e-24	548..809
IcmF_C	PF06744.14	2.8e-10	1058..1145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	MFGKIFNYLKSK
Transmembrane helix	13-35	TFLYILLICFALLLSVLFYIYSP
Outside	36-49	LFAFNDIYIFSSAL
Transmembrane helix	50-72	TRIQVLIVVWLLIFFIFLYRPLI
Inside	73-453	HLIQSLKNEKHIQYKEIKQEVTKAFRRAKRNYFIALNDAKSMWKRKLYLKNIPLVIIIGNEGAGKSSFINYANIQYPLSESLTSYKKFHKSTNNFNLYISQKGALLDTEGNYFAQENFRQTSNTDEIAEDNLEKNKDFLIKKGVWNQFLHFLNTNTFHSKLNGIVLIIDVHAFLQNPKQYEQNVLQYLSKRVSDCEENLGLQLPIYIVFNKIDLLEGMRTYWDIFDESIANKMLGLTLDKNSSKLELQNTFTELSNSLLYTFMRRNQSLHSLEDKNLALLFLKQLDVLFALAIDFILQAKEQNAFKNKSYIRGVYFTSAYQENVPRNYLLDAVCEKYEIKKPPAKSATKQNKRSYFVQGLLEHIFLKDAHLSSMIKTSWQT
Transmembrane helix	454-476	FKLAFGTISICIFGYVFCTYLIH
Outside	477-1184	KTYKEVDKSHITFNSITSLLANTSSYEHLTLQEKTELMLHLKAILKNYPSLFEKPSIIQYFSLNFSYQGFLPARDFYYAINEEVIGKTLLREMEQILLHNTDQTTLIETIYMYQSLYDEAYINKPLLANWVIKNWQPFAKYKIPKDDFITAIEDLAIGNLSHTQPKDTQALHIAKTQIMQLEQQQRIYTIMAFRNSLKKQSFFNLKDKVGTAFYMIFEHTDKLSQIDKTYTKDGLMEFLSNLDTNIQTAIKIESWAYNEKMQYQALTEQAQRDLYTHIISIYLNDYKQRWEELLQAISPKQYTDKNNILTQLQILSESTNPLNSLIKIVNDNTHLNDTLLLNYAYGLGLPSSDIKAQFTYISNSFKDYYDFIGEKSLLLSQIDSFNDIKKQSSTEEIKEILAKDIQNMSGKITEFTKDDTKNIKDKLIYILEGSNDETDAFVELKRNTAMLPHALKQYYNEISTLSWNVIEKSTYALLNNAWKKEVYDTFINDISPFYPFNAYSDKSLPLNTFKDFFGNTGTLQSFYTQYLSKILQKKGNTYVPNPAYSETIKLSAQFISFFNQISNLNSIFDNNNNLTLNFFIQCLGLSSDFSSLDISYNNQTLHYDHTLNPKIQIIIEQFNSSTELRLSVNDYYQMPQYNKIYSGEWAWLRFIKDITPAQNTGNTLYFENNKQWYFDFSITPNRLELLHLSRILTHFNMPQLITQQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9458	-	-

Protein sequence: 1185 a.a.

						>T6CP008406 NC_004917:243237-246791 [Helicobacter hepaticus ATCC 51449] [TssM]
MFGKIFNYLKSKTFLYILLICFALLLSVLFYIYSPLFAFNDIYIFSSALTRIQVLIVVWLLIFFIFLYRPLIHLIQSLKN
EKHIQYKEIKQEVTKAFRRAKRNYFIALNDAKSMWKRKLYLKNIPLVIIIGNEGAGKSSFINYANIQYPLSESLTSYKKF
HKSTNNFNLYISQKGALLDTEGNYFAQENFRQTSNTDEIAEDNLEKNKDFLIKKGVWNQFLHFLNTNTFHSKLNGIVLII
DVHAFLQNPKQYEQNVLQYLSKRVSDCEENLGLQLPIYIVFNKIDLLEGMRTYWDIFDESIANKMLGLTLDKNSSKLELQ
NTFTELSNSLLYTFMRRNQSLHSLEDKNLALLFLKQLDVLFALAIDFILQAKEQNAFKNKSYIRGVYFTSAYQENVPRNY
LLDAVCEKYEIKKPPAKSATKQNKRSYFVQGLLEHIFLKDAHLSSMIKTSWQTFKLAFGTISICIFGYVFCTYLIHKTYK
EVDKSHITFNSITSLLANTSSYEHLTLQEKTELMLHLKAILKNYPSLFEKPSIIQYFSLNFSYQGFLPARDFYYAINEEV
IGKTLLREMEQILLHNTDQTTLIETIYMYQSLYDEAYINKPLLANWVIKNWQPFAKYKIPKDDFITAIEDLAIGNLSHTQ
PKDTQALHIAKTQIMQLEQQQRIYTIMAFRNSLKKQSFFNLKDKVGTAFYMIFEHTDKLSQIDKTYTKDGLMEFLSNLDT
NIQTAIKIESWAYNEKMQYQALTEQAQRDLYTHIISIYLNDYKQRWEELLQAISPKQYTDKNNILTQLQILSESTNPLNS
LIKIVNDNTHLNDTLLLNYAYGLGLPSSDIKAQFTYISNSFKDYYDFIGEKSLLLSQIDSFNDIKKQSSTEEIKEILAKD
IQNMSGKITEFTKDDTKNIKDKLIYILEGSNDETDAFVELKRNTAMLPHALKQYYNEISTLSWNVIEKSTYALLNNAWKK
EVYDTFINDISPFYPFNAYSDKSLPLNTFKDFFGNTGTLQSFYTQYLSKILQKKGNTYVPNPAYSETIKLSAQFISFFNQ
ISNLNSIFDNNNNLTLNFFIQCLGLSSDFSSLDISYNNQTLHYDHTLNPKIQIIIEQFNSSTELRLSVNDYYQMPQYNKI
YSGEWAWLRFIKDITPAQNTGNTLYFENNKQWYFDFSITPNRLELLHLSRILTHFNMPQLITQQ*					

Nucleotide sequence: 3555 bp

						>T6CP008406 NC_004917:243237-246791 [Helicobacter hepaticus ATCC 51449] [TssM]
ATGTTTGGCAAGATATTTAATTATCTCAAATCAAAAACTTTTCTCTATATTTTACTTATATGTTTTGCGTTGCTTTTAAG
TGTACTTTTTTACATATACTCGCCCTTATTTGCATTTAATGATATTTATATTTTTAGTAGCGCATTAACAAGAATCCAAG
TGCTTATTGTCGTATGGCTTTTAATCTTTTTTATCTTTCTTTATAGACCACTTATTCATCTTATTCAATCGCTCAAAAAT
GAGAAACACATACAGTACAAGGAAATCAAACAAGAAGTTACAAAGGCATTCCGCAGGGCTAAAAGAAATTATTTCATTGC
TCTAAATGACGCAAAGAGTATGTGGAAAAGAAAACTCTATCTTAAAAATATCCCTTTAGTGATTATTATTGGTAATGAGG
GAGCAGGCAAAAGCTCGTTTATCAATTATGCGAATATACAATATCCATTGAGTGAAAGCTTGACATCTTATAAGAAATTT
CACAAATCTACCAACAACTTCAATCTTTACATTTCTCAAAAGGGTGCATTGCTTGATACAGAAGGTAATTATTTTGCACA
AGAGAACTTTCGGCAAACAAGTAATACCGATGAAATTGCTGAAGATAATTTAGAAAAGAATAAAGATTTTTTAATCAAAA
AGGGCGTATGGAATCAATTCTTACACTTCCTTAATACTAATACCTTTCATAGCAAACTTAATGGCATTGTGCTTATTATT
GATGTGCATGCTTTTTTACAGAATCCAAAGCAATATGAACAAAATGTCTTACAATATCTAAGCAAAAGAGTGAGTGATTG
CGAAGAAAATCTAGGCTTGCAACTCCCCATTTATATTGTTTTTAATAAAATTGACTTGTTAGAGGGTATGCGGACATATT
GGGACATCTTTGATGAAAGCATTGCAAATAAAATGCTAGGACTTACTTTAGATAAAAATAGTAGCAAATTAGAACTACAA
AATACTTTTACAGAATTAAGCAATTCATTGCTCTATACTTTTATGCGTAGAAATCAATCCTTACATTCACTTGAAGACAA
AAATTTAGCCCTTTTATTTTTAAAGCAACTTGATGTGCTTTTTGCCCTTGCAATAGACTTTATCCTCCAAGCAAAGGAAC
AAAATGCCTTTAAAAACAAATCCTATATCCGCGGCGTATATTTTACCTCCGCATACCAAGAAAATGTCCCCAGAAATTAC
TTGCTTGATGCTGTGTGTGAAAAATACGAAATCAAAAAACCTCCGGCAAAATCCGCAACCAAGCAAAATAAGCGAAGCTA
CTTTGTGCAGGGTTTGTTAGAACATATTTTTCTTAAAGATGCACATCTAAGCAGTATGATTAAAACCTCTTGGCAAACTT
TCAAACTCGCCTTTGGAACTATAAGTATATGTATATTTGGATATGTTTTTTGCACTTATTTAATCCATAAAACCTATAAA
GAAGTAGATAAAAGCCATATCACATTTAATAGCATTACTTCATTACTTGCCAATACAAGCTCTTATGAACATCTTACCCT
GCAAGAAAAAACCGAACTTATGCTTCATCTTAAAGCCATACTCAAAAACTATCCATCTCTATTTGAAAAGCCCTCCATTA
TACAGTATTTTTCACTGAATTTCTCCTATCAAGGATTTTTGCCTGCAAGAGACTTTTATTATGCTATCAATGAAGAAGTG
ATTGGCAAAACATTGCTTAGAGAAATGGAGCAGATTCTCTTACACAATACCGATCAAACCACACTTATTGAAACCATCTA
TATGTATCAATCACTCTATGATGAAGCATATATAAATAAACCTCTTTTGGCAAATTGGGTTATAAAGAATTGGCAACCTT
TTGCTAAATATAAAATTCCAAAAGATGATTTTATTACCGCTATTGAAGATTTAGCTATAGGCAATCTCTCACATACACAA
CCAAAAGATACACAAGCGCTACACATAGCAAAAACTCAAATTATGCAATTAGAGCAACAACAGAGAATCTATACCATTAT
GGCTTTTAGAAATAGCCTAAAAAAACAAAGCTTTTTTAACCTTAAAGATAAGGTAGGCACAGCATTTTATATGATATTTG
AGCATACTGATAAATTAAGTCAAATTGACAAAACCTATACAAAAGATGGTCTAATGGAGTTTTTAAGTAATTTAGATACC
AATATACAAACTGCTATCAAAATAGAATCTTGGGCGTATAATGAAAAAATGCAATACCAAGCCCTCACAGAACAAGCACA
AAGAGATTTATACACACATATTATTAGTATTTACCTTAATGATTATAAACAAAGATGGGAAGAATTACTTCAGGCTATAT
CTCCTAAGCAATATACCGACAAAAACAATATACTCACACAATTACAAATCTTATCAGAATCTACCAATCCACTCAATAGT
CTCATTAAAATCGTAAATGATAATACCCACCTAAACGACACTCTTTTGCTTAATTACGCATACGGCTTAGGATTGCCAAG
CTCAGATATTAAAGCACAATTTACATATATTAGTAACTCCTTTAAAGATTATTATGACTTCATAGGAGAAAAATCCTTAC
TTCTCTCTCAAATAGATTCCTTTAATGACATAAAAAAGCAAAGTAGCACGGAAGAAATAAAAGAGATACTCGCTAAAGAT
ATACAAAATATGAGTGGTAAAATTACAGAATTTACAAAAGATGATACAAAAAACATAAAAGATAAGCTTATATATATCCT
TGAAGGAAGTAATGATGAAACTGACGCATTTGTAGAGCTAAAGCGAAACACAGCAATGTTGCCTCATGCGTTAAAACAAT
ACTACAATGAGATTTCTACACTCTCTTGGAACGTTATAGAAAAAAGCACCTATGCACTTCTAAATAATGCATGGAAAAAA
GAGGTGTATGATACTTTTATAAATGATATCTCCCCTTTTTATCCTTTCAATGCTTACTCGGATAAATCCTTGCCACTCAA
CACTTTTAAAGATTTTTTTGGCAATACAGGCACATTACAATCTTTTTATACGCAATATCTAAGTAAAATTTTGCAAAAAA
AAGGCAATACCTATGTGCCAAATCCTGCCTATAGCGAGACGATAAAGCTTTCTGCTCAATTTATAAGTTTTTTTAACCAA
ATAAGCAATCTCAATAGCATATTTGATAATAACAATAACCTTACACTTAACTTTTTTATACAATGCCTTGGTTTATCATC
AGATTTTAGCTCTCTTGATATAAGCTATAACAACCAGACTTTACACTATGATCACACGCTTAATCCTAAAATCCAGATTA
TTATTGAACAATTCAATAGTAGCACGGAGTTACGTTTGAGCGTGAATGACTATTACCAAATGCCTCAATACAATAAAATT
TATAGTGGCGAATGGGCGTGGCTTAGATTCATAAAAGACATTACACCTGCTCAAAATACAGGCAACACTCTTTACTTTGA
AAATAATAAACAATGGTATTTTGATTTTAGCATTACTCCAAATAGGCTAGAACTTTTACATTTAAGCCGTATTCTAACGC
ATTTTAATATGCCTCAACTTATCACTCAACAATAA					