Detailed information of component protein


Summary

Component ID T6CP008395
Component protein type TssA
T6SS ID (Type) T6SS00037 experimental (Type i2)
Strain Escherichia coli CFT073
Replicon chromosome
Sequence Protein sequence (459 a.a.); Nucleotide sequence (1377 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) C_RS16155
Coordinate (Strand) 3247914..3249290 (+)
NCBI ID WP_000157817.1
RefSeq NC_004431
Uniprot ID A0A0H2VBQ9_ECOL6, E2QQT0_ECOLX, A0A0H3EKR9_ECO8N
KEGG ID ecc:c3403, eln:NRG857_13885
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 3.2e-42 306..449
ImpA_N PF06812.14 3.5e-12 24..112
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-458 MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWRQVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQVHSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDLLAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDKDASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPLPLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDALWPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTGSELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILLDTKVEQSQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9822 - -


  Protein sequence: 459 a.a.    

>T6CP008395 NC_004431:3247914-3249290 [Escherichia coli CFT073] [TssA]
MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWRQVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGM
ADGLGSLDILLQRWADFWPVQVHSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDKDASPPVPSPEISRQRTWPIFMA
GMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPLPLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWP
LEQGYRQLRQLDALWPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTGSELKTAVY
GIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILLDTKVEQSQ*

  Nucleotide sequence: 1377 bp    

>T6CP008395 NC_004431:3247914-3249290 [Escherichia coli CFT073] [TssA]
ATGGCAAGTAACGCGAATTTTATCAGCCAGTTCGTCATGGGCGGCGATCCCTGTACTTATAAGGAATCCGGTGAACTGCA
GGCTGAAATGAGTAAACTGACTCACCCGGCCCGACCGGATGTGGACTGGCGTCAGGTGGAAAAACTCTGCCTCGCGCTGT
TCCGGCAAAATGGCGTGGAACTACAGACGCTGGTCTGTTACGTACTGGCGATAACCAGACGGCAGGGGCTGGCAGGGATG
GCAGACGGACTCGGTTCACTGGATATACTGCTCCAGCGCTGGGCTGACTTCTGGCCGGTACAGGTACATTCCAGAATATC
ACTGCTCAGCTGGGTCACAGAAAAAATGCAGCAGGCACTGAGAACGCTGGATATTCAGTATCAGGATCTGCCGCAGATCT
ACCGTTGTGTACAGCATCTTTCCGCCATCGAAACCACGCTGCAACAGTGTGAACTGTGGCATATGACGAAACTGGATCTA
CTGGCCGGGCAGTTTCGCAATACCGCATTGCGTCTGGAACGGCTGGCGCCTCAGGGAGCGGAAACCACTATCACTCCCCC
TGAATTACCCCGCCGGGAAATGAATCAACCGAAAAAGTCAGAGGAAAGTCCACAGCCGGTTTTTGCAACCAGATCCGTTC
AGCAAAACGATAAGGATGCCAGTCCACCCGTTCCATCCCCTGAAATCTCCCGGCAGCGGACATGGCCGATATTTATGGCC
GGAATGGTTGTGATGGCCGGTCTCGGCGGAACAGGATTATGGGGCTGGTCGCAGCTTAATCAGCCGGACGCGCTAATCCA
GCGAATACAACTGTCTGTCATGCCGTTGCCGCTGTCGCTGGAGAGCGGCGAACTGGCAAAGCTGGATGTAAAGGATAAGG
CGCTGCTGGCTCAGGACAGAACAATTGCGGCAAGTCAGATGCAACTGGAGCAGTTAAACAAATTGCCTGCCCGCTGGCCA
CTGGAGCAGGGATATCGCCAGCTACGCCAGCTTGATGCCCTGTGGCCGGATAATCCTCAGGTCAGGGCGCTGAACGCGCA
GTGGCGCAAACAGCGGGAGCTGAGCGCCCTGTCTGCTGAGGCACTGAATGGCTATGCTCAGGCGCAGAGCCAGCTACAGC
GCCTGTCGGCGCAGCTGGATGCACTGGATGAACGTAAGGGGAGATATCTGACCGGTTCGGAACTAAAAACGGCGGTGTAC
GGCATCCGGCAGTCGTTAAAGGAGCCGCCGCTGGAAGAACTGCTTCGGCAACTGGAAGAGCAAAAACAGACCGGAGAGGT
TTCGCCAACGCTGTTGACGCAAATTGATACCCGGTTAAATCAGTTGTTGAATCGCTATGTCATTTTACTGGATACGAAGG
TGGAACAAAGTCAGTAA