SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP008394
Component protein type	TssJ
T6SS ID (Type)	T6SS00037 (Type i2)
Strain	Escherichia coli CFT073
Replicon	chromosome
Sequence	Protein sequence (179 a.a.); Nucleotide sequence (537 bp)
Reference	[1] Lloyd AL, Henderson TA, Vigil PD, et al. Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 2009 Jun;191(11):3469-81. doi: 10.1128/JB.01717-08. Epub 2009 Mar 27. PMID: 19329634 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Leiman PG, Basler M, Ramagopal UA, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4154-9. doi: 10.1073/pnas.0813360106. Epub 2009 Feb 27. PMID: 19251641

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C_RS16145
Coordinate (Strand)	3246946..3247482 (+)
NCBI ID	WP_000484008.1
RefSeq	NC_004431
Uniprot ID	S1PP52_ECOLX, L3Q082_ECOLX, A0A0E2L1L1_ECOU3, A0A0H2VCL0_ECOL6, A0A454A719_ECOL5, Q1R7K7_ECOUT, A0A0H2Z2G8_ECOK1, E2QQS8_ECOLX, E0IWK9_ECOLW, B7MLF5_ECO45, A0A7U9BMB2_ECOLX, A0A1X3K5H1_ECOLX, A0A7U9ASU3_ECOLX, A0A0H3ELM3_ECO8N, I2WW57_ECOLX
KEGG ID	elw:ECW_m3055, ecv:APECO1_3696, ecc:c3401, ecp:ECP_2821, eln:NRG857_13875, ecz:ECS88_3104, eci:UTI89_C3208
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.9e-27	49..166

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-178	MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYKQIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKDVLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKPRSIELMRSDLRLLPLKDK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9983	pos: 23-24. LSG-CG.	MFPIRFKRPALLCMAMLTVVLSG

Protein sequence: 179 a.a.

						>T6CP008394 NC_004431:3246946-3247482 [Escherichia coli CFT073] [TssJ]
MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYKQIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTR
EDFDKADYDTLFMQEEKTLEKDVLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
RSIELMRSDLRLLPLKDK*					

Nucleotide sequence: 537 bp

						>T6CP008394 NC_004431:3246946-3247482 [Escherichia coli CFT073] [TssJ]
ATGTTTCCTATTCGTTTTAAACGTCCGGCGTTGCTCTGTATGGCGATGCTGACGGTTGTTCTGAGTGGCTGCGGCCTGAT
TCAGAAAGTGGTGGATGAATCGAAAAGCGTGGCCTCAGCCGTTTTCTACAAACAAATCAAAATACTGCATCTCGATTTCT
TCTCCCGCAGCGCCCTGAATACGGATGCGGAAGATACGCCGCTTTCCACGATGGTGCATGTCTGGCAACTGAAAACCCGC
GAAGATTTTGACAAGGCGGATTACGACACCCTGTTTATGCAGGAAGAGAAGACGCTGGAGAAGGACGTACTGGCAAAACA
CACCGTCTGGGTAAAACCGGAAGGCACGGCATCCCTGAATGTGCCGCTGGATAAAGAGACGCAGTTTGTCGCCATTATTG
GGCAGTTTTATCACCCTGATGAAAAAAGCGACAGCTGGCGTCTGGTGATCAAAAGGGACGAACTGGAGGCCGACAAGCCG
CGCTCGATTGAACTGATGAGAAGCGACCTGCGACTGCTGCCTCTCAAGGATAAATAA					