Detailed information of component protein


Summary

Component ID T6CP008077
Component protein type TssI
T6SS ID (Type) T6SS00764 experimental (Type i3)
Strain Pseudomonas protegens CHA0
Replicon chromosome
Sequence Protein sequence (646 a.a.); Nucleotide sequence (1938 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PFLCHA0_RS30185
Coordinate (Strand) 6716694..6718631 (+)
NCBI ID WP_011064262.1
RefSeq NC_021237
Uniprot ID A0A2C9EW35_PSEPH, Q4K3N1_PSEF5
KEGG ID pfl:PFL_6094, pprc:PFLCHA0_c60530
PDB ID 7Q5P
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 5.6e-103 30..329
Phage_base_V PF04717.14 5.9e-15 386..453
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-645 MLFQQSTRLAQVNCPLGPDVLLLKSLGGGEELGRLFDYQLQLASSDANIDLNQLLGKPMGLSVQLDGGGQRYFHGIVARCSQNIDTGQFASYEVTLRPWLWLLSRTSDCRIFQNLSIPQIIKQVFRDLGFSDFEDALSRPYREWEYCVQYRETSFDFVSRLMEQEGIYYFFRHEKDRHVVVLADAYGAHSSVPGYASVPYYPRDEQQRERDHMFDWHLAQEVQPGSLELNDYDFQRPSARIDVRSAMPRPHSAGDYPLYDYPGTYVQSSDGEHYAQTRIEALQSLHERIELSGNARGLGVGNLFSLTGFSRQDQNREYLIVSIRYYLVQESLESGAGGGSAQFESHLTCIDAQQSFRPLATTHKPMVQGPQTARVVGPAGEEIWTDQYGRVKVHFHWDRHDQSNENSSCWIRVSQAWAGKNWGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQTVPYDLPANATQSGMKSRSSKGGSPANFNEIRMEDKKGAEQLYIHAERNQDIVVEVNESHSVGNNRNKSIGHDEYVTIGNKRTRIVQHVDELRVGEKKLDSVGQSYVIEVGERLRLVCGASILELNASGQINLCGVNISVHASADAQINTGGVLHLNNGGGPGTTTEGQGVQGAISAKAKAPFSAPKG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9890 - -


  Protein sequence: 646 a.a.    

>T6CP008077 NC_021237:6716694-6718631 [Pseudomonas protegens CHA0] [TssI]
MLFQQSTRLAQVNCPLGPDVLLLKSLGGGEELGRLFDYQLQLASSDANIDLNQLLGKPMGLSVQLDGGGQRYFHGIVARC
SQNIDTGQFASYEVTLRPWLWLLSRTSDCRIFQNLSIPQIIKQVFRDLGFSDFEDALSRPYREWEYCVQYRETSFDFVSR
LMEQEGIYYFFRHEKDRHVVVLADAYGAHSSVPGYASVPYYPRDEQQRERDHMFDWHLAQEVQPGSLELNDYDFQRPSAR
IDVRSAMPRPHSAGDYPLYDYPGTYVQSSDGEHYAQTRIEALQSLHERIELSGNARGLGVGNLFSLTGFSRQDQNREYLI
VSIRYYLVQESLESGAGGGSAQFESHLTCIDAQQSFRPLATTHKPMVQGPQTARVVGPAGEEIWTDQYGRVKVHFHWDRH
DQSNENSSCWIRVSQAWAGKNWGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQTVPYDLPANATQSGMKSRSSKGG
SPANFNEIRMEDKKGAEQLYIHAERNQDIVVEVNESHSVGNNRNKSIGHDEYVTIGNKRTRIVQHVDELRVGEKKLDSVG
QSYVIEVGERLRLVCGASILELNASGQINLCGVNISVHASADAQINTGGVLHLNNGGGPGTTTEGQGVQGAISAKAKAPF
SAPKG*

  Nucleotide sequence: 1938 bp    

>T6CP008077 NC_021237:6716694-6718631 [Pseudomonas protegens CHA0] [TssI]
ATGTTATTTCAGCAATCCACACGACTGGCACAGGTCAACTGCCCCCTGGGACCGGACGTCCTGCTGCTCAAGAGCCTGGG
CGGCGGCGAGGAGCTGGGGCGGCTGTTCGACTACCAGCTGCAACTGGCCTCCAGTGACGCCAACATCGACCTCAACCAGT
TGCTGGGCAAGCCCATGGGCCTCAGCGTACAGCTGGACGGCGGCGGCCAGCGGTACTTCCACGGCATCGTCGCGCGCTGC
AGCCAGAACATCGACACCGGCCAGTTCGCCAGCTACGAGGTGACGCTGCGCCCCTGGCTGTGGCTGCTGAGTCGCACCTC
CGACTGCCGGATTTTCCAGAACCTGAGCATCCCGCAGATCATCAAGCAGGTGTTCCGCGACCTGGGCTTTTCCGATTTCG
AAGACGCCCTGAGCCGCCCCTACCGGGAGTGGGAGTACTGCGTGCAGTACCGCGAGACCAGCTTCGATTTCGTCAGCCGG
CTGATGGAACAGGAAGGTATCTACTACTTCTTCCGCCACGAGAAGGACCGCCACGTGGTGGTGCTGGCCGACGCCTATGG
CGCTCACAGCAGCGTGCCCGGCTACGCCTCGGTGCCCTACTACCCGCGAGACGAGCAGCAGCGCGAGCGCGACCACATGT
TCGACTGGCACCTGGCCCAGGAGGTGCAGCCCGGCTCGCTGGAGCTCAACGACTATGATTTCCAGCGCCCCAGCGCCCGG
ATCGACGTGCGCTCGGCCATGCCGCGGCCCCACAGCGCCGGCGACTACCCGCTCTACGACTACCCCGGCACCTACGTGCA
GAGCAGCGACGGCGAGCACTATGCCCAGACCCGTATCGAGGCCCTGCAAAGCCTGCATGAGCGCATCGAGCTCAGCGGCA
ACGCCCGGGGGTTGGGGGTGGGCAACCTGTTCAGCCTCACCGGCTTCAGCCGCCAGGACCAGAACCGCGAATACCTGATC
GTCAGCATCCGCTACTACCTGGTCCAGGAAAGCCTGGAGAGCGGCGCTGGCGGCGGTTCGGCGCAGTTCGAGAGCCACCT
GACCTGCATCGACGCCCAGCAGAGCTTCCGCCCCCTGGCCACCACCCACAAGCCCATGGTCCAGGGCCCGCAGACCGCGC
GAGTGGTGGGCCCGGCCGGGGAAGAGATCTGGACCGACCAGTACGGCCGGGTCAAGGTGCACTTCCACTGGGACCGCCAC
GACCAGTCCAACGAAAACAGCTCGTGCTGGATTCGCGTGTCCCAGGCCTGGGCCGGGAAGAACTGGGGCTCGATGCAGAT
CCCGCGCATCGGCCAGGAAGTCATCGTCAGCTTCCTGGAAGGCGATCCCGATCGCCCGATCATCACCGGCCGGGTCTACA
ACGCCGAACAGACCGTGCCCTACGACCTGCCCGCCAACGCCACCCAGAGCGGCATGAAGAGCCGTTCGAGCAAGGGCGGC
AGCCCGGCCAACTTCAATGAAATCCGCATGGAAGACAAAAAGGGCGCGGAGCAGCTGTACATCCACGCCGAGCGCAACCA
GGACATCGTGGTCGAGGTCAACGAAAGCCATTCGGTGGGCAACAACCGCAACAAGAGCATCGGCCACGACGAGTACGTGA
CCATCGGCAACAAGCGCACGCGCATCGTTCAGCACGTGGATGAGCTGCGGGTCGGCGAGAAGAAGCTCGACAGCGTCGGC
CAGAGCTATGTGATCGAGGTCGGCGAGCGCCTGCGCCTGGTATGCGGGGCCAGCATCCTGGAGCTCAACGCCAGCGGCCA
GATCAACCTGTGCGGGGTCAACATCAGCGTGCATGCCTCGGCCGATGCCCAGATCAATACCGGTGGCGTGCTGCACCTGA
ACAACGGCGGCGGCCCGGGCACCACCACCGAGGGCCAGGGTGTGCAGGGCGCCATCAGCGCCAAGGCCAAAGCCCCCTTT
TCCGCCCCCAAAGGCTAA