Detailed information of component protein
Summary
| Component ID | T6CP008070 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS00764  (Type i3) |
| Strain | Pseudomonas protegens CHA0 |
| Replicon | chromosome |
| Sequence | Protein sequence (172 a.a.); Nucleotide sequence (516 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | PFLCHA0_RS30150 |
| Coordinate (Strand) | 6707715..6708230 (+) |
| NCBI ID | WP_011064255.1 |
| RefSeq | NC_021237 |
| Uniprot ID | A0A2C9EVT5_PSEPH, A0A1K2AA43_9PSED, A0A2T0Z758_9PSED, Q4K3N8_PSEF5, A0A7U9LBS9_PSEFL |
| KEGG ID | pfl:PFL_6087, pprc:PFLCHA0_c60460 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.2e-56 | 11..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-171 | VAKQSSQKFIARNRAPRVQIEYDVELYGAEKKVQLPFVMGVMADLAGKPVEPLAAVADRKFLEIDVDNFDSRLKAMQPRVAFHVPNELTGEGNLSLDITFESMDDFSPAAVARKVDSLNQLLEARTQLANLLTYMDGKTGAEEIIMKAIKDPALLQALASAPKPADSEPNA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9958 | - | - |
Protein sequence: 172 a.a.
>T6CP008070 NC_021237:6707715-6708230 [Pseudomonas protegens CHA0] [TssB]
VAKQSSQKFIARNRAPRVQIEYDVELYGAEKKVQLPFVMGVMADLAGKPVEPLAAVADRKFLEIDVDNFDSRLKAMQPRV
AFHVPNELTGEGNLSLDITFESMDDFSPAAVARKVDSLNQLLEARTQLANLLTYMDGKTGAEEIIMKAIKDPALLQALAS
APKPADSEPNA*
VAKQSSQKFIARNRAPRVQIEYDVELYGAEKKVQLPFVMGVMADLAGKPVEPLAAVADRKFLEIDVDNFDSRLKAMQPRV
AFHVPNELTGEGNLSLDITFESMDDFSPAAVARKVDSLNQLLEARTQLANLLTYMDGKTGAEEIIMKAIKDPALLQALAS
APKPADSEPNA*
Nucleotide sequence: 516 bp
>T6CP008070 NC_021237:6707715-6708230 [Pseudomonas protegens CHA0] [TssB]
GTGGCGAAGCAAAGTTCTCAAAAGTTCATCGCGCGCAACCGCGCGCCCCGGGTGCAGATCGAGTACGACGTCGAACTCTA
CGGCGCCGAGAAAAAGGTCCAGCTGCCCTTCGTCATGGGCGTGATGGCCGACCTCGCCGGCAAGCCCGTGGAGCCCCTGG
CGGCCGTGGCCGATCGCAAGTTCCTGGAAATCGACGTCGATAACTTCGACTCGCGCCTCAAGGCCATGCAGCCTCGGGTG
GCCTTCCACGTGCCCAACGAACTGACCGGCGAAGGCAACCTGAGCCTGGACATCACCTTCGAGAGCATGGACGACTTCAG
CCCGGCGGCCGTGGCGCGCAAGGTCGATTCGTTGAACCAGTTGCTGGAAGCCCGCACCCAGCTGGCCAACCTGCTGACCT
ACATGGACGGCAAGACCGGCGCCGAAGAAATCATCATGAAAGCCATCAAGGATCCGGCGTTGCTCCAGGCCCTGGCCAGT
GCGCCAAAGCCTGCTGACTCCGAGCCGAACGCGTAA
GTGGCGAAGCAAAGTTCTCAAAAGTTCATCGCGCGCAACCGCGCGCCCCGGGTGCAGATCGAGTACGACGTCGAACTCTA
CGGCGCCGAGAAAAAGGTCCAGCTGCCCTTCGTCATGGGCGTGATGGCCGACCTCGCCGGCAAGCCCGTGGAGCCCCTGG
CGGCCGTGGCCGATCGCAAGTTCCTGGAAATCGACGTCGATAACTTCGACTCGCGCCTCAAGGCCATGCAGCCTCGGGTG
GCCTTCCACGTGCCCAACGAACTGACCGGCGAAGGCAACCTGAGCCTGGACATCACCTTCGAGAGCATGGACGACTTCAG
CCCGGCGGCCGTGGCGCGCAAGGTCGATTCGTTGAACCAGTTGCTGGAAGCCCGCACCCAGCTGGCCAACCTGCTGACCT
ACATGGACGGCAAGACCGGCGCCGAAGAAATCATCATGAAAGCCATCAAGGATCCGGCGTTGCTCCAGGCCCTGGCCAGT
GCGCCAAAGCCTGCTGACTCCGAGCCGAACGCGTAA