Detailed information of component protein
Summary
| Component ID | T6CP008068 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00764  (Type i3) |
| Strain | Pseudomonas protegens CHA0 |
| Replicon | chromosome |
| Sequence | Protein sequence (169 a.a.); Nucleotide sequence (507 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | PFLCHA0_RS30135 |
| Coordinate (Strand) | 6704166..6704672 (-) |
| NCBI ID | WP_011064252.1 |
| RefSeq | NC_021237 |
| Uniprot ID | A0A2C9EW26_PSEPH, A0A1K2AB75_9PSED, A0A2T0Z754_9PSED, Q4K3P1_PSEF5, A0A7U9LCH5_PSEFL |
| KEGG ID | pprc:PFLCHA0_c60430, pfl:PFL_6084 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.2e-30 | 35..150 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-168 | MIQRLLTLVAMLVLAACAKDTPPPGEPAKEAPGATTVELHFQAVSGLNPGPDGQSTPVRVRIFELKNPATFMRADYFALAERAPATLGPDLIDQDEVLVKPGDQFTLKRTLDPATRQVGLLVGYREVDQALWRVGVNTAQRLSNQYQIVLDVRAVSAELVASPSSPAQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9987 | pos: 16-17. LAA-CA. | MIQRLLTLVAMLVLAA |
Protein sequence: 169 a.a.
>T6CP008068 NC_021237:c6704672-6704166 [Pseudomonas protegens CHA0] [TssJ]
MIQRLLTLVAMLVLAACAKDTPPPGEPAKEAPGATTVELHFQAVSGLNPGPDGQSTPVRVRIFELKNPATFMRADYFALA
ERAPATLGPDLIDQDEVLVKPGDQFTLKRTLDPATRQVGLLVGYREVDQALWRVGVNTAQRLSNQYQIVLDVRAVSAELV
ASPSSPAQ*
MIQRLLTLVAMLVLAACAKDTPPPGEPAKEAPGATTVELHFQAVSGLNPGPDGQSTPVRVRIFELKNPATFMRADYFALA
ERAPATLGPDLIDQDEVLVKPGDQFTLKRTLDPATRQVGLLVGYREVDQALWRVGVNTAQRLSNQYQIVLDVRAVSAELV
ASPSSPAQ*
Nucleotide sequence: 507 bp
>T6CP008068 NC_021237:c6704672-6704166 [Pseudomonas protegens CHA0] [TssJ]
ATGATTCAACGATTGCTGACATTGGTTGCCATGCTGGTACTGGCGGCCTGTGCCAAGGACACACCACCTCCCGGCGAACC
GGCCAAGGAAGCGCCCGGCGCCACGACTGTGGAGCTGCATTTCCAGGCGGTCAGCGGGCTCAACCCCGGGCCTGATGGCC
AGAGCACGCCGGTGCGGGTACGGATCTTCGAGCTGAAGAATCCGGCCACCTTCATGCGCGCCGACTACTTCGCCCTGGCG
GAGCGGGCGCCGGCTACCCTCGGCCCGGACCTGATCGACCAGGATGAAGTGCTGGTCAAGCCCGGCGACCAGTTCACCCT
CAAGCGCACCCTGGACCCGGCCACCCGTCAGGTCGGCCTGCTGGTGGGCTATCGCGAAGTGGACCAGGCCCTGTGGCGGG
TCGGGGTCAACACCGCGCAGCGCCTGAGCAACCAATATCAGATCGTCCTCGACGTCCGCGCGGTCAGCGCCGAGCTCGTG
GCATCCCCATCCAGCCCTGCCCAATAG
ATGATTCAACGATTGCTGACATTGGTTGCCATGCTGGTACTGGCGGCCTGTGCCAAGGACACACCACCTCCCGGCGAACC
GGCCAAGGAAGCGCCCGGCGCCACGACTGTGGAGCTGCATTTCCAGGCGGTCAGCGGGCTCAACCCCGGGCCTGATGGCC
AGAGCACGCCGGTGCGGGTACGGATCTTCGAGCTGAAGAATCCGGCCACCTTCATGCGCGCCGACTACTTCGCCCTGGCG
GAGCGGGCGCCGGCTACCCTCGGCCCGGACCTGATCGACCAGGATGAAGTGCTGGTCAAGCCCGGCGACCAGTTCACCCT
CAAGCGCACCCTGGACCCGGCCACCCGTCAGGTCGGCCTGCTGGTGGGCTATCGCGAAGTGGACCAGGCCCTGTGGCGGG
TCGGGGTCAACACCGCGCAGCGCCTGAGCAACCAATATCAGATCGTCCTCGACGTCCGCGCGGTCAGCGCCGAGCTCGTG
GCATCCCCATCCAGCCCTGCCCAATAG