Detailed information of component protein


Summary

Component ID T6CP008066
Component protein type TssL
T6SS ID (Type) T6SS00764 experimental (Type i3)
Strain Pseudomonas protegens CHA0
Replicon chromosome
Sequence Protein sequence (442 a.a.); Nucleotide sequence (1326 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PFLCHA0_RS30125
Coordinate (Strand) 6701484..6702809 (-)
NCBI ID WP_015637481.1
RefSeq NC_021237
Uniprot ID A0A2C9EVY6_PSEPH
KEGG ID pprc:PFLCHA0_c60410
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.3e-68 59..261
OmpA PF00691.22 2.3e-15 331..429
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-241 MHPFDDSPGQPSPPNDRTQFMPRPGGRAPEPGRPAAAPAPSLAMPAAPLPAGQARGLNPLESAAGPLLALLTRLRSTLAHPAPASLRAQLLAYLRQFEERAEAAGVPRNDVLLARYALCTALDEAVLSTPWGSTSDWGKQSLLITVHNEAWGGEKVFQLLEHCLQSPRERLYLLELLYLCMCLGFEGRYRVMIDGRSQLEALRERTAATIRSARGEHERELSPHWRGVSVARDRLAQFMPP
Transmembrane helix 242-264 WVGIAIGVALLLALLFGLRLKLA
Inside 265-441 ADAEPVFKNIHALGEIPVQTLDRPVIQPKIVERPRLAGFLAEDIKAGRVAVEDAVDRSVVTIRGDELFASASASILDDYQPLMLRIADAVRKVKGQVRITGHSDNRPIATLRFPSNWKLSQARAESVLQILAAKTGQPERFSAEGRSDTEPLATNATAEGRARNRRVEISVLAEGVE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.6497 - -


  Protein sequence: 442 a.a.    

>T6CP008066 NC_021237:c6702809-6701484 [Pseudomonas protegens CHA0] [TssL]
MHPFDDSPGQPSPPNDRTQFMPRPGGRAPEPGRPAAAPAPSLAMPAAPLPAGQARGLNPLESAAGPLLALLTRLRSTLAH
PAPASLRAQLLAYLRQFEERAEAAGVPRNDVLLARYALCTALDEAVLSTPWGSTSDWGKQSLLITVHNEAWGGEKVFQLL
EHCLQSPRERLYLLELLYLCMCLGFEGRYRVMIDGRSQLEALRERTAATIRSARGEHERELSPHWRGVSVARDRLAQFMP
PWVGIAIGVALLLALLFGLRLKLAADAEPVFKNIHALGEIPVQTLDRPVIQPKIVERPRLAGFLAEDIKAGRVAVEDAVD
RSVVTIRGDELFASASASILDDYQPLMLRIADAVRKVKGQVRITGHSDNRPIATLRFPSNWKLSQARAESVLQILAAKTG
QPERFSAEGRSDTEPLATNATAEGRARNRRVEISVLAEGVE*

  Nucleotide sequence: 1326 bp    

>T6CP008066 NC_021237:c6702809-6701484 [Pseudomonas protegens CHA0] [TssL]
ATGCATCCTTTCGACGATTCGCCAGGGCAGCCGTCCCCGCCCAACGACCGTACCCAATTCATGCCGCGCCCCGGTGGCCG
TGCTCCCGAGCCCGGGCGCCCGGCTGCTGCGCCCGCGCCGTCGCTGGCCATGCCAGCGGCGCCGCTGCCGGCCGGCCAGG
CCCGTGGCCTGAACCCCCTGGAAAGCGCCGCGGGCCCGCTGCTGGCCTTGCTCACAAGGTTGCGCAGCACCCTGGCGCAC
CCGGCGCCGGCCAGCCTGAGGGCGCAACTGCTGGCCTACCTGCGCCAGTTCGAGGAGCGCGCGGAAGCCGCCGGCGTGCC
GCGCAACGACGTGCTGCTGGCGCGCTATGCGCTCTGTACTGCCCTGGATGAAGCCGTGCTGAGCACGCCCTGGGGCAGCA
CCAGCGACTGGGGCAAGCAGAGCCTACTGATCACCGTGCACAACGAGGCCTGGGGCGGCGAGAAGGTCTTCCAGCTGCTG
GAGCACTGCCTGCAAAGCCCGCGCGAGCGCCTGTACCTGCTGGAGCTGTTGTACCTGTGCATGTGCCTGGGTTTCGAAGG
CCGCTACCGGGTGATGATCGATGGCCGCAGCCAGCTCGAGGCCCTGCGCGAGCGCACCGCGGCCACCATCCGCAGCGCCC
GTGGCGAGCACGAGCGCGAGCTGTCGCCACACTGGCGCGGGGTCAGCGTGGCTCGTGACCGGCTGGCCCAGTTCATGCCG
CCTTGGGTCGGCATCGCCATCGGCGTGGCCCTGCTCCTGGCGCTGCTGTTCGGCCTGCGCCTGAAACTGGCGGCGGACGC
CGAGCCGGTGTTCAAGAACATCCATGCCCTGGGCGAGATCCCGGTGCAGACCCTGGACCGCCCGGTGATCCAGCCGAAGA
TTGTCGAGCGTCCGCGCCTGGCGGGCTTCCTCGCCGAGGACATCAAGGCCGGGCGGGTGGCGGTGGAAGACGCCGTCGAT
CGTTCGGTGGTGACCATCCGTGGCGACGAGCTGTTCGCCTCGGCCAGCGCCAGCATCCTCGACGACTACCAGCCGCTGAT
GCTGCGCATCGCCGACGCGGTGCGCAAGGTCAAGGGCCAGGTGCGGATCACCGGCCACAGCGACAACCGACCGATCGCCA
CCTTGCGCTTCCCGTCCAACTGGAAGCTGTCCCAGGCCCGCGCCGAGTCGGTGTTGCAGATCCTCGCGGCGAAGACCGGG
CAGCCGGAGCGCTTCAGCGCCGAAGGCCGCAGTGACACCGAGCCGCTGGCAACCAATGCCACCGCCGAAGGGCGGGCACG
TAATCGTCGGGTAGAAATCAGCGTATTGGCGGAGGGGGTCGAGTGA